Xylella phage Sano
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5Q8F6|V5Q8F6_9CAUD Tail assembly protein OS=Xylella phage Sano OX=1415148 GN=Sano_51 PE=4 SV=1
MM1 pKa = 7.7 ADD3 pKa = 3.76 EE4 pKa = 4.4 YY5 pKa = 11.08 TPPLSPNIVWVFKK18 pKa = 10.79 GNEE21 pKa = 4.02 YY22 pKa = 10.81 NPPLSPNIEE31 pKa = 4.32 WIFGADD37 pKa = 3.55 DD38 pKa = 4.32 DD39 pKa = 5.37 GGGNEE44 pKa = 4.42 LRR46 pKa = 11.84 KK47 pKa = 9.94 SSYY50 pKa = 10.16 MLLLTMM56 pKa = 5.94
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.176
IPC2_protein 4.126
IPC_protein 3.923
Toseland 3.745
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.668
Solomon 3.872
Lehninger 3.834
Nozaki 4.05
DTASelect 4.19
Thurlkill 3.821
EMBOSS 3.859
Sillero 4.037
Patrickios 3.3
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.954
Protein with the highest isoelectric point:
>tr|V5Q7F2|V5Q7F2_9CAUD Tail assembly protein OS=Xylella phage Sano OX=1415148 GN=Sano_47 PE=4 SV=1
MM1 pKa = 7.65 EE2 pKa = 5.3 IKK4 pKa = 10.33 PRR6 pKa = 11.84 IGADD10 pKa = 2.74 LWHH13 pKa = 7.58 AITQGTTLKK22 pKa = 10.56 VRR24 pKa = 11.84 FILALQSLIFGASLLVRR41 pKa = 11.84 PMPAPTRR48 pKa = 11.84 WALFATLPVYY58 pKa = 10.28 AWSAMLWLSGSLMLWRR74 pKa = 11.84 VLSTRR79 pKa = 11.84 PVTWLAWASNVFALFTWFIICLSYY103 pKa = 11.06 AAFEE107 pKa = 4.27 GWRR110 pKa = 11.84 GLVGTHH116 pKa = 6.36 ILALVMAVFCVLRR129 pKa = 11.84 TEE131 pKa = 4.35 ATRR134 pKa = 11.84 SDD136 pKa = 4.25 LEE138 pKa = 4.35 TAA140 pKa = 4.06
Molecular weight: 15.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.311
IPC_protein 9.955
Toseland 10.014
ProMoST 9.838
Dawson 10.248
Bjellqvist 10.028
Wikipedia 10.467
Rodwell 10.35
Grimsley 10.321
Solomon 10.35
Lehninger 10.321
Nozaki 10.131
DTASelect 9.999
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.189
Patrickios 10.16
IPC_peptide 10.335
IPC2_peptide 9.355
IPC2.peptide.svr19 8.209
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
17774
47
1446
230.8
25.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.375 ± 0.685
0.771 ± 0.107
6.577 ± 0.28
6.712 ± 0.301
3.82 ± 0.172
7.578 ± 0.285
1.935 ± 0.194
4.529 ± 0.158
5.469 ± 0.311
8.09 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.791 ± 0.161
3.747 ± 0.182
4.439 ± 0.271
3.719 ± 0.308
6.757 ± 0.222
5.564 ± 0.211
5.531 ± 0.209
6.83 ± 0.247
1.738 ± 0.158
3.027 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here