Beihai narna-like virus 12
Average proteome isoelectric point is 8.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIB0|A0A1L3KIB0_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 12 OX=1922439 PE=4 SV=1
MM1 pKa = 7.48 SKK3 pKa = 10.62 SDD5 pKa = 3.94 DD6 pKa = 3.69 RR7 pKa = 11.84 RR8 pKa = 11.84 PSDD11 pKa = 3.02 SQYY14 pKa = 11.06 PLRR17 pKa = 11.84 LSVSKK22 pKa = 9.82 TKK24 pKa = 10.67 DD25 pKa = 2.93 EE26 pKa = 3.92 EE27 pKa = 4.15 AFRR30 pKa = 11.84 VGLLQEE36 pKa = 3.94 PRR38 pKa = 11.84 CRR40 pKa = 11.84 KK41 pKa = 9.3 VALLLAPSCKK51 pKa = 9.74 VEE53 pKa = 4.52 SVATTSPADD62 pKa = 3.58 PLKK65 pKa = 10.96 PPGADD70 pKa = 2.7 NWRR73 pKa = 11.84 LSPSDD78 pKa = 3.55 GQIVTTLKK86 pKa = 10.03 IVSIITAAIEE96 pKa = 4.18 DD97 pKa = 3.68 VHH99 pKa = 7.73 DD100 pKa = 3.6 FHH102 pKa = 9.26 LNDD105 pKa = 3.63 RR106 pKa = 11.84 QIVQAVLMWLPYY118 pKa = 10.9 VMADD122 pKa = 2.66 VWGPVLKK129 pKa = 10.49 ARR131 pKa = 11.84 KK132 pKa = 9.58 DD133 pKa = 3.3 ILFAYY138 pKa = 10.1 ALRR141 pKa = 11.84 QEE143 pKa = 5.07 EE144 pKa = 5.46 IPPPWCEE151 pKa = 4.25 CCNQTYY157 pKa = 10.41 ILPGRR162 pKa = 11.84 AGSALRR168 pKa = 11.84 QRR170 pKa = 11.84 VLYY173 pKa = 10.31 NKK175 pKa = 10.33 NEE177 pKa = 4.31 DD178 pKa = 3.26 NRR180 pKa = 11.84 IFANTILHH188 pKa = 5.82 GLKK191 pKa = 10.27 KK192 pKa = 10.58 GMPKK196 pKa = 10.31 LSEE199 pKa = 3.7 AHH201 pKa = 6.85 VIDD204 pKa = 5.49 GYY206 pKa = 10.87 LKK208 pKa = 10.07 HH209 pKa = 6.9 RR210 pKa = 11.84 EE211 pKa = 3.96 RR212 pKa = 11.84 LSTDD216 pKa = 2.92 KK217 pKa = 11.5 GPVDD221 pKa = 3.33 PALRR225 pKa = 11.84 EE226 pKa = 4.46 CITRR230 pKa = 11.84 TCRR233 pKa = 11.84 EE234 pKa = 3.67 IFGGMKK240 pKa = 10.03 FPEE243 pKa = 4.38 TTPVLTQNVSSKK255 pKa = 11.18 SSLHH259 pKa = 5.58 SNRR262 pKa = 11.84 SEE264 pKa = 3.83 GGALSEE270 pKa = 4.53 IFSEE274 pKa = 4.28 EE275 pKa = 4.55 DD276 pKa = 3.02 PLEE279 pKa = 4.25 YY280 pKa = 10.68 YY281 pKa = 10.51 GEE283 pKa = 4.57 CFTEE287 pKa = 4.04 EE288 pKa = 4.08 TGPLSIKK295 pKa = 8.02 TNKK298 pKa = 8.27 FAAMEE303 pKa = 3.96 KK304 pKa = 10.02 LGQIRR309 pKa = 11.84 EE310 pKa = 3.94 RR311 pKa = 11.84 TARR314 pKa = 11.84 VLRR317 pKa = 11.84 SDD319 pKa = 3.94 EE320 pKa = 4.09 GSKK323 pKa = 10.78 LSSHH327 pKa = 6.15 WQTQAILEE335 pKa = 4.1 PLKK338 pKa = 10.92 CRR340 pKa = 11.84 MITKK344 pKa = 10.67 GEE346 pKa = 4.09 LRR348 pKa = 11.84 WNAAWSDD355 pKa = 3.26 IQKK358 pKa = 10.69 EE359 pKa = 4.12 MFRR362 pKa = 11.84 RR363 pKa = 11.84 LGRR366 pKa = 11.84 YY367 pKa = 7.39 EE368 pKa = 3.87 QFKK371 pKa = 9.07 LTRR374 pKa = 11.84 GHH376 pKa = 6.78 EE377 pKa = 4.0 IEE379 pKa = 4.71 KK380 pKa = 10.16 QDD382 pKa = 3.43 SLMFQVSGDD391 pKa = 3.95 PLDD394 pKa = 4.93 LDD396 pKa = 4.02 GDD398 pKa = 3.92 QFYY401 pKa = 11.39 VAGDD405 pKa = 3.74 FSAATDD411 pKa = 3.79 EE412 pKa = 4.68 LKK414 pKa = 10.92 ADD416 pKa = 3.62 ATRR419 pKa = 11.84 AAIEE423 pKa = 4.47 GFTDD427 pKa = 3.6 PVLRR431 pKa = 11.84 NLLTVNLLNGLINYY445 pKa = 7.01 PPKK448 pKa = 10.69 YY449 pKa = 10.07 EE450 pKa = 4.32 IDD452 pKa = 3.71 PVHH455 pKa = 5.81 QANGQLMGSIFSFPLLCAINLAIYY479 pKa = 9.93 RR480 pKa = 11.84 YY481 pKa = 6.88 TWEE484 pKa = 4.36 SRR486 pKa = 11.84 DD487 pKa = 3.22 KK488 pKa = 11.38 SGFRR492 pKa = 11.84 KK493 pKa = 10.01 PIDD496 pKa = 3.67 SLPVLINGDD505 pKa = 4.18 DD506 pKa = 3.2 IAFRR510 pKa = 11.84 TTRR513 pKa = 11.84 WFYY516 pKa = 11.48 EE517 pKa = 3.92 DD518 pKa = 2.9 WKK520 pKa = 10.93 KK521 pKa = 11.15 NSAEE525 pKa = 4.03 AGLIASRR532 pKa = 11.84 GKK534 pKa = 10.84 CYY536 pKa = 8.38 FTKK539 pKa = 10.55 KK540 pKa = 10.2 FFLVNSRR547 pKa = 11.84 LIHH550 pKa = 5.02 STPTGILTQAIPYY563 pKa = 9.21 VNYY566 pKa = 10.88 GLVTGQKK573 pKa = 9.95 KK574 pKa = 10.65 GEE576 pKa = 4.26 GDD578 pKa = 3.62 DD579 pKa = 3.9 DD580 pKa = 4.07 LARR583 pKa = 11.84 SRR585 pKa = 11.84 RR586 pKa = 11.84 EE587 pKa = 3.54 KK588 pKa = 10.53 LACLEE593 pKa = 4.01 GALRR597 pKa = 11.84 DD598 pKa = 3.66 LSKK601 pKa = 11.41 DD602 pKa = 2.91 FDD604 pKa = 3.94 EE605 pKa = 5.64 RR606 pKa = 11.84 SEE608 pKa = 3.93 ILKK611 pKa = 10.38 RR612 pKa = 11.84 ATARR616 pKa = 11.84 AMKK619 pKa = 10.2 RR620 pKa = 11.84 GDD622 pKa = 3.18 IRR624 pKa = 11.84 ISGISKK630 pKa = 8.33 TQLGLPSVEE639 pKa = 4.4 IDD641 pKa = 3.21 DD642 pKa = 5.19 DD643 pKa = 3.73 EE644 pKa = 4.72 ARR646 pKa = 11.84 RR647 pKa = 11.84 YY648 pKa = 7.85 LTYY651 pKa = 10.83 ASRR654 pKa = 11.84 IRR656 pKa = 11.84 DD657 pKa = 3.54 RR658 pKa = 11.84 GQKK661 pKa = 9.64 EE662 pKa = 3.73 PSAGHH667 pKa = 6.16 TGLAPFIFKK676 pKa = 10.31 FPRR679 pKa = 11.84 VKK681 pKa = 10.17 TFGNLRR687 pKa = 11.84 TKK689 pKa = 7.39 QHH691 pKa = 6.35 KK692 pKa = 10.3 AVDD695 pKa = 4.31 LVKK698 pKa = 10.23 HH699 pKa = 5.93 LAMVGEE705 pKa = 4.46 KK706 pKa = 9.93 KK707 pKa = 9.94 EE708 pKa = 3.77 KK709 pKa = 9.73 RR710 pKa = 11.84 ARR712 pKa = 11.84 EE713 pKa = 3.93 EE714 pKa = 3.71 EE715 pKa = 3.94 ALMEE719 pKa = 4.16 VLSAQRR725 pKa = 11.84 KK726 pKa = 8.74 RR727 pKa = 11.84 GFAFDD732 pKa = 3.38 IGRR735 pKa = 11.84 LPEE738 pKa = 4.12 SSEE741 pKa = 3.86 EE742 pKa = 3.44 ILYY745 pKa = 10.12 NKK747 pKa = 8.89 KK748 pKa = 10.39 RR749 pKa = 11.84 KK750 pKa = 9.17
Molecular weight: 84.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.288
IPC2_protein 8.244
IPC_protein 8.097
Toseland 8.624
ProMoST 8.741
Dawson 9.077
Bjellqvist 9.048
Wikipedia 9.165
Rodwell 9.238
Grimsley 8.902
Solomon 9.136
Lehninger 9.107
Nozaki 9.224
DTASelect 8.902
Thurlkill 9.033
EMBOSS 9.238
Sillero 9.224
Patrickios 4.863
IPC_peptide 9.121
IPC2_peptide 7.717
IPC2.peptide.svr19 7.665
Protein with the highest isoelectric point:
>tr|A0A1L3KIB0|A0A1L3KIB0_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 12 OX=1922439 PE=4 SV=1
MM1 pKa = 7.48 SKK3 pKa = 10.62 SDD5 pKa = 3.94 DD6 pKa = 3.69 RR7 pKa = 11.84 RR8 pKa = 11.84 PSDD11 pKa = 3.02 SQYY14 pKa = 11.06 PLRR17 pKa = 11.84 LSVSKK22 pKa = 9.82 TKK24 pKa = 10.67 DD25 pKa = 2.93 EE26 pKa = 3.92 EE27 pKa = 4.15 AFRR30 pKa = 11.84 VGLLQEE36 pKa = 3.94 PRR38 pKa = 11.84 CRR40 pKa = 11.84 KK41 pKa = 9.3 VALLLAPSCKK51 pKa = 9.74 VEE53 pKa = 4.52 SVATTSPADD62 pKa = 3.58 PLKK65 pKa = 10.96 PPGADD70 pKa = 2.7 NWRR73 pKa = 11.84 LSPSDD78 pKa = 3.55 GQIVTTLKK86 pKa = 10.03 IVSIITAAIEE96 pKa = 4.18 DD97 pKa = 3.68 VHH99 pKa = 7.73 DD100 pKa = 3.6 FHH102 pKa = 9.26 LNDD105 pKa = 3.63 RR106 pKa = 11.84 QIVQAVLMWLPYY118 pKa = 10.9 VMADD122 pKa = 2.66 VWGPVLKK129 pKa = 10.49 ARR131 pKa = 11.84 KK132 pKa = 9.58 DD133 pKa = 3.3 ILFAYY138 pKa = 10.1 ALRR141 pKa = 11.84 QEE143 pKa = 5.07 EE144 pKa = 5.46 IPPPWCEE151 pKa = 4.25 CCNQTYY157 pKa = 10.41 ILPGRR162 pKa = 11.84 AGSALRR168 pKa = 11.84 QRR170 pKa = 11.84 VLYY173 pKa = 10.31 NKK175 pKa = 10.33 NEE177 pKa = 4.31 DD178 pKa = 3.26 NRR180 pKa = 11.84 IFANTILHH188 pKa = 5.82 GLKK191 pKa = 10.27 KK192 pKa = 10.58 GMPKK196 pKa = 10.31 LSEE199 pKa = 3.7 AHH201 pKa = 6.85 VIDD204 pKa = 5.49 GYY206 pKa = 10.87 LKK208 pKa = 10.07 HH209 pKa = 6.9 RR210 pKa = 11.84 EE211 pKa = 3.96 RR212 pKa = 11.84 LSTDD216 pKa = 2.92 KK217 pKa = 11.5 GPVDD221 pKa = 3.33 PALRR225 pKa = 11.84 EE226 pKa = 4.46 CITRR230 pKa = 11.84 TCRR233 pKa = 11.84 EE234 pKa = 3.67 IFGGMKK240 pKa = 10.03 FPEE243 pKa = 4.38 TTPVLTQNVSSKK255 pKa = 11.18 SSLHH259 pKa = 5.58 SNRR262 pKa = 11.84 SEE264 pKa = 3.83 GGALSEE270 pKa = 4.53 IFSEE274 pKa = 4.28 EE275 pKa = 4.55 DD276 pKa = 3.02 PLEE279 pKa = 4.25 YY280 pKa = 10.68 YY281 pKa = 10.51 GEE283 pKa = 4.57 CFTEE287 pKa = 4.04 EE288 pKa = 4.08 TGPLSIKK295 pKa = 8.02 TNKK298 pKa = 8.27 FAAMEE303 pKa = 3.96 KK304 pKa = 10.02 LGQIRR309 pKa = 11.84 EE310 pKa = 3.94 RR311 pKa = 11.84 TARR314 pKa = 11.84 VLRR317 pKa = 11.84 SDD319 pKa = 3.94 EE320 pKa = 4.09 GSKK323 pKa = 10.78 LSSHH327 pKa = 6.15 WQTQAILEE335 pKa = 4.1 PLKK338 pKa = 10.92 CRR340 pKa = 11.84 MITKK344 pKa = 10.67 GEE346 pKa = 4.09 LRR348 pKa = 11.84 WNAAWSDD355 pKa = 3.26 IQKK358 pKa = 10.69 EE359 pKa = 4.12 MFRR362 pKa = 11.84 RR363 pKa = 11.84 LGRR366 pKa = 11.84 YY367 pKa = 7.39 EE368 pKa = 3.87 QFKK371 pKa = 9.07 LTRR374 pKa = 11.84 GHH376 pKa = 6.78 EE377 pKa = 4.0 IEE379 pKa = 4.71 KK380 pKa = 10.16 QDD382 pKa = 3.43 SLMFQVSGDD391 pKa = 3.95 PLDD394 pKa = 4.93 LDD396 pKa = 4.02 GDD398 pKa = 3.92 QFYY401 pKa = 11.39 VAGDD405 pKa = 3.74 FSAATDD411 pKa = 3.79 EE412 pKa = 4.68 LKK414 pKa = 10.92 ADD416 pKa = 3.62 ATRR419 pKa = 11.84 AAIEE423 pKa = 4.47 GFTDD427 pKa = 3.6 PVLRR431 pKa = 11.84 NLLTVNLLNGLINYY445 pKa = 7.01 PPKK448 pKa = 10.69 YY449 pKa = 10.07 EE450 pKa = 4.32 IDD452 pKa = 3.71 PVHH455 pKa = 5.81 QANGQLMGSIFSFPLLCAINLAIYY479 pKa = 9.93 RR480 pKa = 11.84 YY481 pKa = 6.88 TWEE484 pKa = 4.36 SRR486 pKa = 11.84 DD487 pKa = 3.22 KK488 pKa = 11.38 SGFRR492 pKa = 11.84 KK493 pKa = 10.01 PIDD496 pKa = 3.67 SLPVLINGDD505 pKa = 4.18 DD506 pKa = 3.2 IAFRR510 pKa = 11.84 TTRR513 pKa = 11.84 WFYY516 pKa = 11.48 EE517 pKa = 3.92 DD518 pKa = 2.9 WKK520 pKa = 10.93 KK521 pKa = 11.15 NSAEE525 pKa = 4.03 AGLIASRR532 pKa = 11.84 GKK534 pKa = 10.84 CYY536 pKa = 8.38 FTKK539 pKa = 10.55 KK540 pKa = 10.2 FFLVNSRR547 pKa = 11.84 LIHH550 pKa = 5.02 STPTGILTQAIPYY563 pKa = 9.21 VNYY566 pKa = 10.88 GLVTGQKK573 pKa = 9.95 KK574 pKa = 10.65 GEE576 pKa = 4.26 GDD578 pKa = 3.62 DD579 pKa = 3.9 DD580 pKa = 4.07 LARR583 pKa = 11.84 SRR585 pKa = 11.84 RR586 pKa = 11.84 EE587 pKa = 3.54 KK588 pKa = 10.53 LACLEE593 pKa = 4.01 GALRR597 pKa = 11.84 DD598 pKa = 3.66 LSKK601 pKa = 11.41 DD602 pKa = 2.91 FDD604 pKa = 3.94 EE605 pKa = 5.64 RR606 pKa = 11.84 SEE608 pKa = 3.93 ILKK611 pKa = 10.38 RR612 pKa = 11.84 ATARR616 pKa = 11.84 AMKK619 pKa = 10.2 RR620 pKa = 11.84 GDD622 pKa = 3.18 IRR624 pKa = 11.84 ISGISKK630 pKa = 8.33 TQLGLPSVEE639 pKa = 4.4 IDD641 pKa = 3.21 DD642 pKa = 5.19 DD643 pKa = 3.73 EE644 pKa = 4.72 ARR646 pKa = 11.84 RR647 pKa = 11.84 YY648 pKa = 7.85 LTYY651 pKa = 10.83 ASRR654 pKa = 11.84 IRR656 pKa = 11.84 DD657 pKa = 3.54 RR658 pKa = 11.84 GQKK661 pKa = 9.64 EE662 pKa = 3.73 PSAGHH667 pKa = 6.16 TGLAPFIFKK676 pKa = 10.31 FPRR679 pKa = 11.84 VKK681 pKa = 10.17 TFGNLRR687 pKa = 11.84 TKK689 pKa = 7.39 QHH691 pKa = 6.35 KK692 pKa = 10.3 AVDD695 pKa = 4.31 LVKK698 pKa = 10.23 HH699 pKa = 5.93 LAMVGEE705 pKa = 4.46 KK706 pKa = 9.93 KK707 pKa = 9.94 EE708 pKa = 3.77 KK709 pKa = 9.73 RR710 pKa = 11.84 ARR712 pKa = 11.84 EE713 pKa = 3.93 EE714 pKa = 3.71 EE715 pKa = 3.94 ALMEE719 pKa = 4.16 VLSAQRR725 pKa = 11.84 KK726 pKa = 8.74 RR727 pKa = 11.84 GFAFDD732 pKa = 3.38 IGRR735 pKa = 11.84 LPEE738 pKa = 4.12 SSEE741 pKa = 3.86 EE742 pKa = 3.44 ILYY745 pKa = 10.12 NKK747 pKa = 8.89 KK748 pKa = 10.39 RR749 pKa = 11.84 KK750 pKa = 9.17
Molecular weight: 84.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.288
IPC2_protein 8.244
IPC_protein 8.097
Toseland 8.624
ProMoST 8.741
Dawson 9.077
Bjellqvist 9.048
Wikipedia 9.165
Rodwell 9.238
Grimsley 8.902
Solomon 9.136
Lehninger 9.107
Nozaki 9.224
DTASelect 8.902
Thurlkill 9.033
EMBOSS 9.238
Sillero 9.224
Patrickios 4.863
IPC_peptide 9.121
IPC2_peptide 7.717
IPC2.peptide.svr19 7.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
750
750
750
750.0
84.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.467 ± 0.0
1.6 ± 0.0
6.267 ± 0.0
7.2 ± 0.0
4.0 ± 0.0
6.4 ± 0.0
1.733 ± 0.0
5.733 ± 0.0
7.333 ± 0.0
10.133 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.733 ± 0.0
3.067 ± 0.0
5.067 ± 0.0
3.333 ± 0.0
8.133 ± 0.0
6.933 ± 0.0
5.2 ± 0.0
4.533 ± 0.0
1.333 ± 0.0
2.8 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here