Mycobacterium virus Yoshi
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1BSL3|G1BSL3_9CAUD Uncharacterized protein OS=Mycobacterium virus Yoshi OX=1034150 GN=106 PE=4 SV=1
MM1 pKa = 7.93 SDD3 pKa = 3.41 PQSTRR8 pKa = 11.84 DD9 pKa = 3.4 RR10 pKa = 11.84 LIANIEE16 pKa = 3.97 NTIWNALEE24 pKa = 4.13 QQDD27 pKa = 3.91 GPYY30 pKa = 10.15 VDD32 pKa = 5.56 RR33 pKa = 11.84 EE34 pKa = 4.09 MGMVDD39 pKa = 3.51 ASGAGLDD46 pKa = 3.74 MTAVAAAVADD56 pKa = 3.53 IFVDD60 pKa = 4.98 SEE62 pKa = 5.14 DD63 pKa = 3.74 DD64 pKa = 4.06 CSWCHH69 pKa = 6.11 SPCAVGGWSEE79 pKa = 3.98 PVDD82 pKa = 3.5 VGSIVQSEE90 pKa = 4.2 PNQEE94 pKa = 4.43 GITDD98 pKa = 3.52 VV99 pKa = 3.5
Molecular weight: 10.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 3.516
IPC_protein 3.478
Toseland 3.274
ProMoST 3.681
Dawson 3.49
Bjellqvist 3.656
Wikipedia 3.465
Rodwell 3.325
Grimsley 3.185
Solomon 3.465
Lehninger 3.414
Nozaki 3.63
DTASelect 3.846
Thurlkill 3.35
EMBOSS 3.465
Sillero 3.605
Patrickios 1.799
IPC_peptide 3.452
IPC2_peptide 3.579
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|G1BSH8|G1BSH8_9CAUD Uncharacterized protein OS=Mycobacterium virus Yoshi OX=1034150 GN=71 PE=4 SV=1
MM1 pKa = 7.33 TGRR4 pKa = 11.84 VLTPIEE10 pKa = 4.13 VEE12 pKa = 4.43 KK13 pKa = 10.5 VAWWTQLGWTAAQIADD29 pKa = 3.91 KK30 pKa = 10.73 LGCTTRR36 pKa = 11.84 TVSRR40 pKa = 11.84 ARR42 pKa = 11.84 AKK44 pKa = 10.82 SGVAKK49 pKa = 9.61 PKK51 pKa = 10.37 PPALSAEE58 pKa = 4.39 VLAEE62 pKa = 4.13 AARR65 pKa = 11.84 MLADD69 pKa = 3.78 GASQNEE75 pKa = 4.29 VARR78 pKa = 11.84 TLGVGQSTISAHH90 pKa = 6.15 FRR92 pKa = 11.84 GQGWTRR98 pKa = 11.84 EE99 pKa = 3.97 QSIEE103 pKa = 3.86 WVSFLRR109 pKa = 11.84 RR110 pKa = 11.84 YY111 pKa = 10.26 RR112 pKa = 11.84 GVAA115 pKa = 2.99
Molecular weight: 12.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.516
IPC_protein 10.189
Toseland 10.789
ProMoST 10.452
Dawson 10.847
Bjellqvist 10.54
Wikipedia 11.052
Rodwell 11.038
Grimsley 10.877
Solomon 11.008
Lehninger 10.979
Nozaki 10.774
DTASelect 10.54
Thurlkill 10.774
EMBOSS 11.199
Sillero 10.789
Patrickios 10.818
IPC_peptide 11.008
IPC2_peptide 9.458
IPC2.peptide.svr19 8.749
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
116
0
116
18912
33
1183
163.0
17.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.014 ± 0.356
1.338 ± 0.152
6.446 ± 0.206
5.97 ± 0.291
3.014 ± 0.139
8.74 ± 0.568
2.2 ± 0.153
4.479 ± 0.171
3.633 ± 0.181
7.482 ± 0.234
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.21 ± 0.117
3.231 ± 0.162
5.716 ± 0.217
3.781 ± 0.183
6.662 ± 0.439
5.785 ± 0.237
6.208 ± 0.268
7.308 ± 0.264
2.295 ± 0.129
2.49 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here