Tolypocladium capitatum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Tolypocladium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K3QR88|A0A2K3QR88_9HYPO Uncharacterized protein OS=Tolypocladium capitatum OX=45235 GN=TCAP_00028 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 4.43DD4 pKa = 4.42EE5 pKa = 4.57NLSIYY10 pKa = 10.65DD11 pKa = 3.91EE12 pKa = 4.54IEE14 pKa = 4.33IEE16 pKa = 5.51DD17 pKa = 3.74MAFDD21 pKa = 4.0EE22 pKa = 4.84AQQLYY27 pKa = 9.98HH28 pKa = 6.21YY29 pKa = 7.58PCPCGDD35 pKa = 3.63RR36 pKa = 11.84FQIALEE42 pKa = 4.1DD43 pKa = 3.88LRR45 pKa = 11.84DD46 pKa = 3.71EE47 pKa = 4.31QDD49 pKa = 2.98IAVCPSCSLMIRR61 pKa = 11.84VIFDD65 pKa = 4.38LDD67 pKa = 4.33DD68 pKa = 4.16LPKK71 pKa = 10.34PPPSGNSGRR80 pKa = 11.84QIPVAII86 pKa = 4.59

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K3QI35|A0A2K3QI35_9HYPO Aquaporin PIP2-7 OS=Tolypocladium capitatum OX=45235 GN=TCAP_02886 PE=3 SV=1
SS1 pKa = 6.84SSSPSRR7 pKa = 11.84TATSRR12 pKa = 11.84SAPRR16 pKa = 11.84ASSSKK21 pKa = 9.16STPSAQPPTSSSSTSIPSASTSRR44 pKa = 11.84TTLRR48 pKa = 11.84PGRR51 pKa = 11.84AAPRR55 pKa = 11.84SPTSWSTASAWAAATRR71 pKa = 11.84SSSSTTPAGWPPRR84 pKa = 11.84SVTSATSASRR94 pKa = 11.84SASASPRR101 pKa = 11.84VAATCC106 pKa = 3.98

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7532

102

7634

3983078

8

4938

521.8

57.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.755 ± 0.026

1.258 ± 0.01

5.996 ± 0.02

5.874 ± 0.023

3.432 ± 0.016

7.414 ± 0.023

2.48 ± 0.011

4.064 ± 0.018

4.387 ± 0.024

8.797 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.009

3.197 ± 0.013

6.452 ± 0.033

3.956 ± 0.023

7.065 ± 0.023

7.937 ± 0.029

5.516 ± 0.016

6.32 ± 0.023

1.387 ± 0.009

2.443 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski