Prolixibacter denitrificans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Prolixibacter

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8CFW4|A0A2P8CFW4_9BACT MoxR-like ATPase OS=Prolixibacter denitrificans OX=1541063 GN=CLV93_103260 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.56NIFTPLFLILLITIFSNKK20 pKa = 8.94SFAQTGPPTPPDD32 pKa = 4.17PDD34 pKa = 4.28SNDD37 pKa = 3.4TEE39 pKa = 4.21NALFDD44 pKa = 4.29NDD46 pKa = 5.35PIPEE50 pKa = 4.34FGSVTFSPSPMTDD63 pKa = 2.88TEE65 pKa = 4.48VTGNNTISASISDD78 pKa = 4.13DD79 pKa = 3.83GEE81 pKa = 4.37VISALVLYY89 pKa = 7.41GTTSGDD95 pKa = 3.39LTKK98 pKa = 10.89SFALSYY104 pKa = 11.16SSGTTWKK111 pKa = 10.63ADD113 pKa = 3.56LPVLEE118 pKa = 5.79DD119 pKa = 3.06GTYY122 pKa = 10.07YY123 pKa = 10.71YY124 pKa = 10.21QIKK127 pKa = 10.19AADD130 pKa = 3.6NSQQEE135 pKa = 4.64VVSTEE140 pKa = 3.5ASFEE144 pKa = 4.13ITAGTPVAPADD155 pKa = 4.07LQASAIDD162 pKa = 3.77YY163 pKa = 11.09NRR165 pKa = 11.84FTLEE169 pKa = 3.84WQPSYY174 pKa = 11.02QATSYY179 pKa = 10.96YY180 pKa = 10.53LIISTEE186 pKa = 3.85EE187 pKa = 4.46DD188 pKa = 3.77YY189 pKa = 11.76SNPIVDD195 pKa = 3.83GDD197 pKa = 3.61IGNVTSYY204 pKa = 10.71EE205 pKa = 4.0ATSVQFSTTYY215 pKa = 10.46YY216 pKa = 9.09YY217 pKa = 10.17QLKK220 pKa = 10.28AIRR223 pKa = 11.84LMDD226 pKa = 5.23DD227 pKa = 3.46DD228 pKa = 5.65AVMEE232 pKa = 4.46SEE234 pKa = 3.99ISSGEE239 pKa = 4.22VTTLDD244 pKa = 3.31QPAPEE249 pKa = 4.16VTVSVEE255 pKa = 4.0SLPDD259 pKa = 3.81FGDD262 pKa = 3.62VVSGEE267 pKa = 4.1NSATSSYY274 pKa = 10.8TVTAQYY280 pKa = 11.12LRR282 pKa = 11.84DD283 pKa = 3.98NLVVTPPTGFEE294 pKa = 4.03VSTDD298 pKa = 3.31EE299 pKa = 5.28TNWQGNASPLSLAGTDD315 pKa = 4.26GEE317 pKa = 4.74LSTTIYY323 pKa = 10.86VRR325 pKa = 11.84FAPDD329 pKa = 3.25TPGATSGDD337 pKa = 3.42ITHH340 pKa = 6.58EE341 pKa = 4.23TTEE344 pKa = 4.1LPEE347 pKa = 4.28TVLVALSGTALEE359 pKa = 4.54TEE361 pKa = 4.55PANHH365 pKa = 5.96VSNFRR370 pKa = 11.84LAGQNGNTFHH380 pKa = 7.53LSWDD384 pKa = 3.71EE385 pKa = 4.13NNGEE389 pKa = 4.07EE390 pKa = 4.42AAEE393 pKa = 4.99GYY395 pKa = 10.79LLLFTQTTVQTPVDD409 pKa = 4.06GEE411 pKa = 4.56TPIEE415 pKa = 5.26DD416 pKa = 3.37IDD418 pKa = 4.4FSDD421 pKa = 4.55GEE423 pKa = 4.17ALIVIDD429 pKa = 5.02KK430 pKa = 8.31DD431 pKa = 3.39TTAAEE436 pKa = 4.45VYY438 pKa = 10.26IPGSEE443 pKa = 4.21TRR445 pKa = 11.84WYY447 pKa = 8.1TQIIPYY453 pKa = 9.77RR454 pKa = 11.84GSDD457 pKa = 2.87EE458 pKa = 4.29TTNVKK463 pKa = 9.43TDD465 pKa = 3.29GTVPEE470 pKa = 4.41ITLITPLDD478 pKa = 4.33DD479 pKa = 6.28LPNAWINEE487 pKa = 3.73FHH489 pKa = 6.97YY490 pKa = 11.19SDD492 pKa = 5.75DD493 pKa = 4.38GDD495 pKa = 3.88DD496 pKa = 3.69HH497 pKa = 7.34AQVEE501 pKa = 4.8IVLEE505 pKa = 4.21NADD508 pKa = 4.04NYY510 pKa = 11.15SLEE513 pKa = 4.18DD514 pKa = 4.51FILIHH519 pKa = 6.21YY520 pKa = 8.77NGAGGTIIDD529 pKa = 4.39EE530 pKa = 4.72FNFADD535 pKa = 3.71NASVSVQGAFSIILIDD551 pKa = 5.06CGTLQKK557 pKa = 11.14GPDD560 pKa = 3.79GLAISYY566 pKa = 7.19QHH568 pKa = 7.12RR569 pKa = 11.84IVQFISYY576 pKa = 9.3EE577 pKa = 3.9DD578 pKa = 3.55SFIATEE584 pKa = 4.36GDD586 pKa = 3.14ATGITSIEE594 pKa = 4.07IAPQEE599 pKa = 4.46TTTTPPNFSIQLTGSGSYY617 pKa = 10.67YY618 pKa = 10.68EE619 pKa = 5.04DD620 pKa = 4.13FTWQDD625 pKa = 3.32PAEE628 pKa = 4.33SSFGAVNAGQILTASPNLEE647 pKa = 4.33TTWLGTQDD655 pKa = 4.35NDD657 pKa = 3.69WNNAANWNNGVPDD670 pKa = 3.83EE671 pKa = 4.34TMKK674 pKa = 11.27GIIPAGVDD682 pKa = 3.29HH683 pKa = 6.75YY684 pKa = 11.0PVLTAGVTPKK694 pKa = 10.27TLVLEE699 pKa = 4.62SDD701 pKa = 3.79ASLSGQQYY709 pKa = 10.53LPEE712 pKa = 4.32SCILYY717 pKa = 10.48AKK719 pKa = 10.28RR720 pKa = 11.84ALTAEE725 pKa = 3.94KK726 pKa = 9.86WLFLTPPVEE735 pKa = 4.4DD736 pKa = 4.61NIAAMFEE743 pKa = 4.19PVYY746 pKa = 11.2GMVYY750 pKa = 10.57LITYY754 pKa = 9.04DD755 pKa = 3.28NSQAADD761 pKa = 3.6EE762 pKa = 4.62SAWNYY767 pKa = 10.46VSDD770 pKa = 3.7QTLPLSPMTGYY781 pKa = 11.02GFYY784 pKa = 9.56STRR787 pKa = 11.84EE788 pKa = 3.79EE789 pKa = 4.47LEE791 pKa = 4.09LVFNGSPISGTQSVLLSFEE810 pKa = 4.27DD811 pKa = 3.6AGNNFNFVGNPGLGAIDD828 pKa = 3.64WSTSNQANTTGTAYY842 pKa = 10.3MFDD845 pKa = 4.42PNAKK849 pKa = 8.94TYY851 pKa = 9.25LTIASDD857 pKa = 3.93GTVTNPEE864 pKa = 4.32FTDD867 pKa = 4.62MIPPMQGFFVEE878 pKa = 4.43ATNAGNYY885 pKa = 9.19SVNLDD890 pKa = 3.61DD891 pKa = 6.4CVPTDD896 pKa = 4.44QYY898 pKa = 11.53FLKK901 pKa = 10.73QGVPEE906 pKa = 4.41DD907 pKa = 3.72YY908 pKa = 10.34IVRR911 pKa = 11.84LKK913 pKa = 9.51VTGPNGHH920 pKa = 7.12DD921 pKa = 3.33FALVRR926 pKa = 11.84FDD928 pKa = 3.32QTNNDD933 pKa = 3.41EE934 pKa = 4.83LDD936 pKa = 3.68FNEE939 pKa = 4.66DD940 pKa = 2.99SRR942 pKa = 11.84KK943 pKa = 9.71LVYY946 pKa = 10.58YY947 pKa = 10.35DD948 pKa = 3.61IGVPQLWFSQEE959 pKa = 3.98GEE961 pKa = 4.02KK962 pKa = 10.8LAINQARR969 pKa = 11.84TYY971 pKa = 9.44PASFPLGLYY980 pKa = 10.11ISDD983 pKa = 4.43DD984 pKa = 4.04SEE986 pKa = 4.54LSITGTVSGSFSDD999 pKa = 5.19GITMSLEE1006 pKa = 3.71NTITGEE1012 pKa = 4.13TLDD1015 pKa = 4.63LRR1017 pKa = 11.84SSDD1020 pKa = 3.37TLRR1023 pKa = 11.84YY1024 pKa = 9.4DD1025 pKa = 4.05ASAGEE1030 pKa = 4.13NNSLILHH1037 pKa = 6.69LKK1039 pKa = 9.91NGTGFDD1045 pKa = 3.34EE1046 pKa = 4.66FTNEE1050 pKa = 3.4KK1051 pKa = 9.74VRR1053 pKa = 11.84IYY1055 pKa = 10.85SSGEE1059 pKa = 3.71ILHH1062 pKa = 5.6VTASQILKK1070 pKa = 10.66GKK1072 pKa = 10.61LEE1074 pKa = 3.94VFNFIGQKK1082 pKa = 7.17ITEE1085 pKa = 4.0YY1086 pKa = 10.75SINHH1090 pKa = 5.59TNHH1093 pKa = 6.29ARR1095 pKa = 11.84VQPGTGIYY1103 pKa = 9.84LVRR1106 pKa = 11.84WTDD1109 pKa = 3.31DD1110 pKa = 3.3EE1111 pKa = 4.3QAVVQKK1117 pKa = 10.98VSLRR1121 pKa = 3.66

Molecular weight: 122.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8CFC8|A0A2P8CFC8_9BACT CRP-like cAMP-binding protein OS=Prolixibacter denitrificans OX=1541063 GN=CLV93_10393 PE=4 SV=1
MM1 pKa = 7.54SSLGGMTSFFRR12 pKa = 11.84PGMLKK17 pKa = 10.43GALGRR22 pKa = 11.84YY23 pKa = 8.02VFSCATSYY31 pKa = 11.22RR32 pKa = 11.84LDD34 pKa = 3.16ITAIPVKK41 pKa = 9.82WLIKK45 pKa = 8.67STVAIVSHH53 pKa = 6.24SSEE56 pKa = 4.19APSGVWGGYY65 pKa = 6.77SRR67 pKa = 11.84RR68 pKa = 11.84PLRR71 pKa = 11.84EE72 pKa = 3.59LFDD75 pKa = 5.15PNNEE79 pKa = 4.1TPIQLINRR87 pKa = 11.84IARR90 pKa = 11.84RR91 pKa = 11.84ASSILVVVAEE101 pKa = 4.29MSHH104 pKa = 6.62RR105 pKa = 11.84APLLRR110 pKa = 11.84RR111 pKa = 11.84CYY113 pKa = 10.72SPNFFNSHH121 pKa = 4.16QAKK124 pKa = 10.27IPGEE128 pKa = 4.28VPASCDD134 pKa = 3.26EE135 pKa = 4.02NRR137 pKa = 11.84RR138 pKa = 11.84ASPFFLFARR147 pKa = 11.84IGG149 pKa = 3.34

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3987

0

3987

1385306

29

3557

347.5

39.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.85 ± 0.038

0.726 ± 0.013

5.561 ± 0.033

6.364 ± 0.045

4.954 ± 0.03

6.966 ± 0.033

2.032 ± 0.018

6.849 ± 0.033

6.742 ± 0.039

9.348 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.018

5.179 ± 0.033

4.001 ± 0.022

3.553 ± 0.024

4.517 ± 0.033

6.218 ± 0.03

5.457 ± 0.036

6.721 ± 0.028

1.283 ± 0.018

4.053 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski