Golden silk orbweaver associated circular virus 1
Average proteome isoelectric point is 7.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPA5|A0A346BPA5_9VIRU Putative capsid protein OS=Golden silk orbweaver associated circular virus 1 OX=2293292 PE=4 SV=1
MM1 pKa = 7.37 SKK3 pKa = 10.36 CKK5 pKa = 9.84 RR6 pKa = 11.84 WLFTSYY12 pKa = 11.15 NVVSEE17 pKa = 4.04 PFYY20 pKa = 10.89 DD21 pKa = 4.05 EE22 pKa = 5.54 KK23 pKa = 10.75 IHH25 pKa = 7.24 EE26 pKa = 4.32 YY27 pKa = 10.83 LCFGRR32 pKa = 11.84 EE33 pKa = 4.14 TCPSTNRR40 pKa = 11.84 RR41 pKa = 11.84 HH42 pKa = 5.06 LQGYY46 pKa = 7.61 VAFKK50 pKa = 11.08 NRR52 pKa = 11.84 ITLAGIKK59 pKa = 9.56 KK60 pKa = 8.59 WIPGAHH66 pKa = 6.24 FEE68 pKa = 4.31 RR69 pKa = 11.84 ARR71 pKa = 11.84 GTPTEE76 pKa = 3.88 NRR78 pKa = 11.84 DD79 pKa = 3.38 YY80 pKa = 10.97 CKK82 pKa = 10.5 KK83 pKa = 10.85 DD84 pKa = 2.99 GDD86 pKa = 3.91 FKK88 pKa = 11.21 EE89 pKa = 4.4 WGSVPTVTGRR99 pKa = 11.84 ACKK102 pKa = 10.35 FGDD105 pKa = 3.43 ILHH108 pKa = 6.64 LAEE111 pKa = 5.39 SGNITCIKK119 pKa = 9.24 DD120 pKa = 3.54 TYY122 pKa = 9.26 PGVYY126 pKa = 9.82 LRR128 pKa = 11.84 YY129 pKa = 9.59 KK130 pKa = 9.8 ATLLSSLVCNTTDD143 pKa = 3.74 LKK145 pKa = 11.13 NSCGVWLCGPPRR157 pKa = 11.84 SGKK160 pKa = 10.32 DD161 pKa = 3.25 YY162 pKa = 11.21 AVRR165 pKa = 11.84 QLDD168 pKa = 3.77 NVYY171 pKa = 10.37 MKK173 pKa = 10.06 MLNKK177 pKa = 9.22 WWDD180 pKa = 3.65 GYY182 pKa = 11.21 KK183 pKa = 10.49 NEE185 pKa = 4.52 DD186 pKa = 3.48 NVLISDD192 pKa = 4.54 IEE194 pKa = 4.17 PDD196 pKa = 3.02 HH197 pKa = 6.58 GKK199 pKa = 9.66 WIGYY203 pKa = 7.26 YY204 pKa = 9.84 IKK206 pKa = 9.91 IWCDD210 pKa = 2.98 MYY212 pKa = 11.66 AFNAEE217 pKa = 4.01 IKK219 pKa = 9.89 GGSMKK224 pKa = 9.93 IRR226 pKa = 11.84 PKK228 pKa = 10.47 KK229 pKa = 10.31 IFMTSNYY236 pKa = 10.04 KK237 pKa = 10.19 LDD239 pKa = 3.62 EE240 pKa = 4.36 VFSGEE245 pKa = 3.97 ILSAVAARR253 pKa = 11.84 CNVYY257 pKa = 10.39 DD258 pKa = 3.97 YY259 pKa = 11.93 SNGCDD264 pKa = 3.38 VIVTKK269 pKa = 10.69 RR270 pKa = 11.84 MSPSPSDD277 pKa = 3.63 VFISALKK284 pKa = 9.1 EE285 pKa = 4.15 HH286 pKa = 7.2 EE287 pKa = 5.53 DD288 pKa = 3.57 GLSFTKK294 pKa = 10.3 EE295 pKa = 3.82 VLQTSSCLQEE305 pKa = 3.92 EE306 pKa = 4.93 AISSISEE313 pKa = 4.05 EE314 pKa = 4.15 SSNTCIEE321 pKa = 4.19 EE322 pKa = 3.99
Molecular weight: 36.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.498
IPC2_protein 6.664
IPC_protein 6.649
Toseland 6.44
ProMoST 6.985
Dawson 7.263
Bjellqvist 7.439
Wikipedia 7.19
Rodwell 7.278
Grimsley 6.478
Solomon 7.293
Lehninger 7.322
Nozaki 7.761
DTASelect 7.483
Thurlkill 7.527
EMBOSS 7.541
Sillero 7.79
Patrickios 3.808
IPC_peptide 7.293
IPC2_peptide 7.059
IPC2.peptide.svr19 6.987
Protein with the highest isoelectric point:
>tr|A0A346BPA5|A0A346BPA5_9VIRU Putative capsid protein OS=Golden silk orbweaver associated circular virus 1 OX=2293292 PE=4 SV=1
MM1 pKa = 7.54 VYY3 pKa = 10.09 RR4 pKa = 11.84 SRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 FYY10 pKa = 10.94 KK11 pKa = 10.21 RR12 pKa = 11.84 RR13 pKa = 11.84 LVYY16 pKa = 10.2 KK17 pKa = 9.34 KK18 pKa = 9.1 RR19 pKa = 11.84 QYY21 pKa = 10.78 RR22 pKa = 11.84 RR23 pKa = 11.84 FRR25 pKa = 11.84 RR26 pKa = 11.84 SRR28 pKa = 11.84 RR29 pKa = 11.84 IRR31 pKa = 11.84 VLKK34 pKa = 10.39 NKK36 pKa = 10.16 RR37 pKa = 11.84 SGNIYY42 pKa = 10.25 KK43 pKa = 9.02 LTVKK47 pKa = 10.48 KK48 pKa = 10.82 SFNTTLRR55 pKa = 11.84 FYY57 pKa = 11.4 VDD59 pKa = 3.25 NASQSSVNITKK70 pKa = 9.94 FEE72 pKa = 4.26 RR73 pKa = 11.84 DD74 pKa = 2.82 IQMADD79 pKa = 3.28 FFTNNHH85 pKa = 6.22 EE86 pKa = 3.82 IWDD89 pKa = 4.85 RR90 pKa = 11.84 IHH92 pKa = 7.54 DD93 pKa = 3.57 YY94 pKa = 11.01 HH95 pKa = 7.73 YY96 pKa = 10.99 IKK98 pKa = 10.69 FNYY101 pKa = 9.63 FIIKK105 pKa = 9.83 FPEE108 pKa = 3.69 ITYY111 pKa = 8.23 VTYY114 pKa = 10.89 SGVTPTGIAKK124 pKa = 9.9 QAVTGISSFNTEE136 pKa = 4.07 RR137 pKa = 11.84 YY138 pKa = 7.61 PFHH141 pKa = 6.42 VAWDD145 pKa = 3.78 VEE147 pKa = 4.21 QALDD151 pKa = 4.89 FNAQDD156 pKa = 3.29 ASFDD160 pKa = 3.65 AKK162 pKa = 10.53 EE163 pKa = 3.74 LSEE166 pKa = 4.31 YY167 pKa = 10.41 PFAKK171 pKa = 9.74 KK172 pKa = 10.26 VYY174 pKa = 8.54 PSQKK178 pKa = 8.43 RR179 pKa = 11.84 APYY182 pKa = 9.87 FMWRR186 pKa = 11.84 VPSCWRR192 pKa = 11.84 QYY194 pKa = 10.89 IDD196 pKa = 3.5 TNAVQPLMFNNIPFYY211 pKa = 11.4 NFFQNVTGIKK221 pKa = 9.85 NVRR224 pKa = 11.84 SPTKK228 pKa = 10.15 ILGGHH233 pKa = 5.5 EE234 pKa = 3.99 NWWGNNLPSTVRR246 pKa = 11.84 PDD248 pKa = 3.43 IKK250 pKa = 10.68 PSDD253 pKa = 3.78 SDD255 pKa = 3.97 TVFMQSFLKK264 pKa = 10.85 CEE266 pKa = 5.09 LIASVTFRR274 pKa = 11.84 GVKK277 pKa = 8.06 YY278 pKa = 9.33 TGRR281 pKa = 11.84 NSS283 pKa = 2.97
Molecular weight: 33.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.706
IPC_protein 10.058
Toseland 10.189
ProMoST 9.926
Dawson 10.409
Bjellqvist 10.101
Wikipedia 10.599
Rodwell 10.745
Grimsley 10.482
Solomon 10.423
Lehninger 10.394
Nozaki 10.175
DTASelect 10.101
Thurlkill 10.248
EMBOSS 10.599
Sillero 10.321
Patrickios 10.262
IPC_peptide 10.423
IPC2_peptide 8.873
IPC2.peptide.svr19 8.695
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
605
283
322
302.5
35.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.628 ± 0.022
2.645 ± 1.224
5.289 ± 0.439
5.124 ± 1.228
5.785 ± 1.48
5.289 ± 1.109
1.818 ± 0.032
6.446 ± 0.054
7.934 ± 0.324
5.289 ± 0.886
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.983 ± 0.137
5.95 ± 0.706
4.132 ± 0.292
2.479 ± 0.889
6.777 ± 1.523
8.099 ± 0.429
5.95 ± 0.259
6.281 ± 0.497
2.314 ± 0.123
5.785 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here