Odonata-associated circular virus-16
Average proteome isoelectric point is 7.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UGW2|A0A0B4UGW2_9VIRU Putative capsid protein OS=Odonata-associated circular virus-16 OX=1592116 PE=4 SV=1
MM1 pKa = 6.95 PRR3 pKa = 11.84 KK4 pKa = 9.13 MEE6 pKa = 4.27 SLEE9 pKa = 4.04 SSGGGNSIPPPIKK22 pKa = 9.83 EE23 pKa = 3.69 KK24 pKa = 10.83 RR25 pKa = 11.84 MNQGRR30 pKa = 11.84 RR31 pKa = 11.84 WCFTWNNYY39 pKa = 9.2 DD40 pKa = 4.0 DD41 pKa = 4.71 NVVTAIKK48 pKa = 10.25 EE49 pKa = 4.03 RR50 pKa = 11.84 LEE52 pKa = 4.21 SKK54 pKa = 10.68 DD55 pKa = 2.86 ILRR58 pKa = 11.84 YY59 pKa = 9.54 IVGFEE64 pKa = 3.6 IGEE67 pKa = 4.3 KK68 pKa = 10.36 EE69 pKa = 4.2 KK70 pKa = 10.47 TPHH73 pKa = 5.19 LQGYY77 pKa = 8.31 FEE79 pKa = 4.37 FTGQIARR86 pKa = 11.84 RR87 pKa = 11.84 PSEE90 pKa = 3.43 MWDD93 pKa = 3.25 NYY95 pKa = 10.58 NLGRR99 pKa = 11.84 FFKK102 pKa = 10.92 CKK104 pKa = 8.74 GTAEE108 pKa = 4.14 EE109 pKa = 3.93 NHH111 pKa = 6.76 AYY113 pKa = 10.04 CSKK116 pKa = 10.37 SGNYY120 pKa = 8.19 FQGGDD125 pKa = 3.08 WSILKK130 pKa = 10.28 NKK132 pKa = 9.48 EE133 pKa = 3.77 PLPIEE138 pKa = 4.11 VLKK141 pKa = 11.31 DD142 pKa = 2.97 EE143 pKa = 5.21 DD144 pKa = 4.67 LYY146 pKa = 10.71 PFQKK150 pKa = 10.58 SIIDD154 pKa = 4.09 IIDD157 pKa = 3.73 GPVIKK162 pKa = 10.48 GHH164 pKa = 5.66 IHH166 pKa = 6.99 WICDD170 pKa = 3.45 LKK172 pKa = 10.84 GQNGKK177 pKa = 7.95 TEE179 pKa = 4.5 LLRR182 pKa = 11.84 KK183 pKa = 9.74 LVVEE187 pKa = 4.53 RR188 pKa = 11.84 NLKK191 pKa = 8.25 FTYY194 pKa = 9.98 GGKK197 pKa = 9.91 KK198 pKa = 9.94 ADD200 pKa = 4.45 IINLIYY206 pKa = 10.77 NNKK209 pKa = 9.62 QYY211 pKa = 11.64 YY212 pKa = 8.24 LTTKK216 pKa = 9.38 NAAMVYY222 pKa = 9.86 NLGRR226 pKa = 11.84 SEE228 pKa = 4.79 DD229 pKa = 3.96 LEE231 pKa = 4.41 KK232 pKa = 11.11 VSYY235 pKa = 10.74 TSMEE239 pKa = 4.11 QISDD243 pKa = 3.65 GLISNTKK250 pKa = 9.79 YY251 pKa = 8.95 EE252 pKa = 4.26 TGCFVMNHH260 pKa = 4.9 PHH262 pKa = 7.15 IIVLANGMPSIRR274 pKa = 11.84 SMTMSRR280 pKa = 11.84 WIIYY284 pKa = 9.25 KK285 pKa = 10.33 INDD288 pKa = 3.34 AKK290 pKa = 10.43 EE291 pKa = 3.99 LEE293 pKa = 4.37 RR294 pKa = 11.84 IEE296 pKa = 4.46 YY297 pKa = 9.65 EE298 pKa = 3.98 SHH300 pKa = 6.86 SNLGSPLDD308 pKa = 4.46 DD309 pKa = 5.15 LDD311 pKa = 3.64 NN312 pKa = 4.35
Molecular weight: 36.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.461
IPC2_protein 6.532
IPC_protein 6.693
Toseland 6.722
ProMoST 7.044
Dawson 7.176
Bjellqvist 7.044
Wikipedia 7.146
Rodwell 7.176
Grimsley 6.825
Solomon 7.22
Lehninger 7.234
Nozaki 7.366
DTASelect 7.454
Thurlkill 7.483
EMBOSS 7.527
Sillero 7.571
Patrickios 4.215
IPC_peptide 7.234
IPC2_peptide 6.956
IPC2.peptide.svr19 6.973
Protein with the highest isoelectric point:
>tr|A0A0B4UGW2|A0A0B4UGW2_9VIRU Putative capsid protein OS=Odonata-associated circular virus-16 OX=1592116 PE=4 SV=1
MM1 pKa = 6.85 VRR3 pKa = 11.84 SRR5 pKa = 11.84 SRR7 pKa = 11.84 SRR9 pKa = 11.84 TSYY12 pKa = 9.8 RR13 pKa = 11.84 KK14 pKa = 8.93 KK15 pKa = 9.44 VSVKK19 pKa = 9.85 RR20 pKa = 11.84 RR21 pKa = 11.84 VPRR24 pKa = 11.84 TRR26 pKa = 11.84 KK27 pKa = 9.81 GVTVPVKK34 pKa = 10.55 KK35 pKa = 10.39 YY36 pKa = 7.12 VQKK39 pKa = 10.74 AIHH42 pKa = 5.86 RR43 pKa = 11.84 QIEE46 pKa = 4.38 NKK48 pKa = 9.68 CVQFNGAFTMVNYY61 pKa = 10.84 ADD63 pKa = 4.6 DD64 pKa = 3.53 NTMRR68 pKa = 11.84 GFALSPGTSISIAQGTGSADD88 pKa = 3.39 RR89 pKa = 11.84 TGNRR93 pKa = 11.84 IKK95 pKa = 10.32 IIKK98 pKa = 9.37 GKK100 pKa = 9.7 LVYY103 pKa = 10.31 WMVPTAYY110 pKa = 10.29 NGLLNPQPKK119 pKa = 9.71 PLMVRR124 pKa = 11.84 LWIGYY129 pKa = 9.54 SKK131 pKa = 9.83 TNPTLIPPAGDD142 pKa = 3.08 QAVLFQNGNTASPPNGFINDD162 pKa = 3.65 MLRR165 pKa = 11.84 SVNKK169 pKa = 10.1 DD170 pKa = 2.68 KK171 pKa = 11.1 FVIFRR176 pKa = 11.84 DD177 pKa = 3.53 IKK179 pKa = 10.83 HH180 pKa = 5.93 KK181 pKa = 10.75 LGYY184 pKa = 10.27 EE185 pKa = 4.4 SITGTGFVAGSQYY198 pKa = 10.49 FANNDD203 pKa = 3.17 FKK205 pKa = 11.37 FNIMRR210 pKa = 11.84 TIDD213 pKa = 3.13 ITKK216 pKa = 9.76 YY217 pKa = 10.48 LINNVIYY224 pKa = 10.64 NDD226 pKa = 3.45 TTAIPTSRR234 pKa = 11.84 GLFCWVQIVYY244 pKa = 10.86 ADD246 pKa = 3.78 NNVTTGTIPANFNYY260 pKa = 9.68 FVDD263 pKa = 4.5 LTYY266 pKa = 11.34 EE267 pKa = 4.05 DD268 pKa = 3.87 AA269 pKa = 5.76
Molecular weight: 30.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.248
IPC2_protein 9.677
IPC_protein 9.897
Toseland 10.233
ProMoST 9.926
Dawson 10.423
Bjellqvist 10.101
Wikipedia 10.599
Rodwell 10.862
Grimsley 10.496
Solomon 10.452
Lehninger 10.423
Nozaki 10.218
DTASelect 10.101
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.335
Patrickios 10.467
IPC_peptide 10.452
IPC2_peptide 8.829
IPC2.peptide.svr19 8.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
581
269
312
290.5
33.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.303 ± 1.07
1.205 ± 0.3
5.164 ± 0.215
4.819 ± 2.41
4.131 ± 0.698
7.401 ± 0.22
1.549 ± 0.524
8.434 ± 0.408
7.917 ± 0.556
6.368 ± 0.999
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.926 ± 0.211
7.917 ± 0.412
4.647 ± 0.363
2.926 ± 0.273
5.68 ± 0.658
6.196 ± 0.403
6.196 ± 1.531
5.68 ± 1.625
1.549 ± 0.282
4.991 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here