Odonata-associated circular virus-7
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UH28|A0A0B4UH28_9VIRU Putative capsid protein OS=Odonata-associated circular virus-7 OX=1592127 PE=4 SV=1
MM1 pKa = 7.45 FNLDD5 pKa = 3.55 GAKK8 pKa = 10.17 SCDD11 pKa = 3.69 FQDD14 pKa = 4.09 PCWCEE19 pKa = 3.74 YY20 pKa = 10.4 IRR22 pKa = 11.84 TEE24 pKa = 3.93 GMGYY28 pKa = 10.21 IMDD31 pKa = 4.53 IDD33 pKa = 4.07 GADD36 pKa = 3.07 ITEE39 pKa = 4.22 FQPDD43 pKa = 3.65 EE44 pKa = 4.99 FIDD47 pKa = 4.29 LGDD50 pKa = 3.88 SRR52 pKa = 11.84 DD53 pKa = 3.85 DD54 pKa = 3.46 EE55 pKa = 4.92 TIVISSDD62 pKa = 3.7 SEE64 pKa = 4.07 DD65 pKa = 3.54 DD66 pKa = 3.87 KK67 pKa = 12.01 EE68 pKa = 4.99 DD69 pKa = 3.84 IEE71 pKa = 4.21 WLEE74 pKa = 4.43 EE75 pKa = 3.51 IRR77 pKa = 11.84 KK78 pKa = 7.97 RR79 pKa = 11.84 TRR81 pKa = 11.84 LMKK84 pKa = 10.48 RR85 pKa = 11.84 EE86 pKa = 3.88 IVKK89 pKa = 10.07 AACEE93 pKa = 3.82 QLAEE97 pKa = 4.01 EE98 pKa = 5.49 LINKK102 pKa = 7.94 NN103 pKa = 3.06
Molecular weight: 12.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.268
IPC2_protein 4.012
IPC_protein 3.973
Toseland 3.77
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.795
Grimsley 3.681
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.808
EMBOSS 3.859
Sillero 4.088
Patrickios 3.681
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 4.02
Protein with the highest isoelectric point:
>tr|A0A0B4UHZ3|A0A0B4UHZ3_9VIRU Replication-associated protein OS=Odonata-associated circular virus-7 OX=1592127 PE=3 SV=1
MM1 pKa = 7.32 AAVAGRR7 pKa = 11.84 FALRR11 pKa = 11.84 SFARR15 pKa = 11.84 YY16 pKa = 8.51 FPQAMRR22 pKa = 11.84 YY23 pKa = 6.4 MARR26 pKa = 11.84 NPGQGGRR33 pKa = 11.84 LLRR36 pKa = 11.84 SSAKK40 pKa = 9.17 YY41 pKa = 10.15 ARR43 pKa = 11.84 AAAPVAGLAANAVRR57 pKa = 11.84 YY58 pKa = 9.45 VSKK61 pKa = 10.68 KK62 pKa = 9.34 SKK64 pKa = 10.38 QYY66 pKa = 11.27 LYY68 pKa = 11.19 LKK70 pKa = 9.86 NLKK73 pKa = 10.04 KK74 pKa = 10.7 KK75 pKa = 9.95 IVSSRR80 pKa = 11.84 GKK82 pKa = 10.14 KK83 pKa = 8.69 NQPSVFKK90 pKa = 10.43 KK91 pKa = 10.44 RR92 pKa = 11.84 RR93 pKa = 11.84 ITTQGVSGKK102 pKa = 10.02 RR103 pKa = 11.84 LEE105 pKa = 4.23 LSRR108 pKa = 11.84 KK109 pKa = 9.15 YY110 pKa = 10.73 VDD112 pKa = 3.64 PFSFYY117 pKa = 10.24 NRR119 pKa = 11.84 KK120 pKa = 9.29 RR121 pKa = 11.84 VVKK124 pKa = 10.07 IMKK127 pKa = 9.93 VYY129 pKa = 9.04 GTEE132 pKa = 3.99 QTDD135 pKa = 3.58 TCCYY139 pKa = 10.05 VEE141 pKa = 4.34 AAVTEE146 pKa = 4.11 PRR148 pKa = 11.84 AWILNMWKK156 pKa = 10.49 ALVRR160 pKa = 11.84 KK161 pKa = 9.76 LSQKK165 pKa = 10.82 AGLQIYY171 pKa = 9.23 SCNEE175 pKa = 4.14 AISMKK180 pKa = 9.32 TLGVGGVDD188 pKa = 4.16 LEE190 pKa = 4.52 MVLYY194 pKa = 8.21 TQNQANVSAPSITVSFADD212 pKa = 3.87 GTTVTSLAEE221 pKa = 4.63 AIAQPFYY228 pKa = 10.79 SYY230 pKa = 11.6 SSGSTNAGVGSVLSLVEE247 pKa = 4.07 PNYY250 pKa = 10.83 LVLQRR255 pKa = 11.84 KK256 pKa = 9.26 SSVVNFTYY264 pKa = 10.55 QIVNLQLDD272 pKa = 3.85 NVLVEE277 pKa = 5.54 GKK279 pKa = 9.59 LCCIMKK285 pKa = 9.85 LQNQNQSNTGGTDD298 pKa = 3.31 SEE300 pKa = 4.77 DD301 pKa = 3.74 VSNHH305 pKa = 5.59 PLVGKK310 pKa = 10.17 LYY312 pKa = 10.23 YY313 pKa = 10.79 FNGLPGTKK321 pKa = 9.76 VPNQDD326 pKa = 3.51 PGSLEE331 pKa = 3.86 NNIFRR336 pKa = 11.84 RR337 pKa = 11.84 FPADD341 pKa = 3.21 RR342 pKa = 11.84 HH343 pKa = 5.83 LKK345 pKa = 9.43 ILKK348 pKa = 9.54 DD349 pKa = 3.54 ANLEE353 pKa = 4.02 QEE355 pKa = 4.2 LRR357 pKa = 11.84 EE358 pKa = 4.47 PISPISFWNCIGCTNFKK375 pKa = 9.97 LQPGEE380 pKa = 3.69 IRR382 pKa = 11.84 TFTFWDD388 pKa = 3.64 QVKK391 pKa = 9.92 SQPFLKK397 pKa = 10.34 YY398 pKa = 9.84 LKK400 pKa = 10.22 KK401 pKa = 10.52 FRR403 pKa = 11.84 LEE405 pKa = 4.1 YY406 pKa = 9.33 GTNAPAQLQDD416 pKa = 3.55 QYY418 pKa = 12.0 NLNYY422 pKa = 10.22 CPLKK426 pKa = 10.12 FAMVGLEE433 pKa = 3.91 EE434 pKa = 4.54 QIQSSSSNNLTIGYY448 pKa = 9.0 EE449 pKa = 3.93 IEE451 pKa = 4.19 SRR453 pKa = 11.84 CASMFKK459 pKa = 10.15 EE460 pKa = 4.11 LRR462 pKa = 11.84 PNFCRR467 pKa = 11.84 TEE469 pKa = 4.14 TEE471 pKa = 4.32 TKK473 pKa = 9.46 QFPVIVV479 pKa = 3.74
Molecular weight: 53.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.893
IPC2_protein 9.165
IPC_protein 9.077
Toseland 9.692
ProMoST 9.472
Dawson 9.97
Bjellqvist 9.692
Wikipedia 10.145
Rodwell 10.292
Grimsley 10.058
Solomon 9.984
Lehninger 9.94
Nozaki 9.794
DTASelect 9.663
Thurlkill 9.809
EMBOSS 10.131
Sillero 9.897
Patrickios 5.105
IPC_peptide 9.984
IPC2_peptide 8.448
IPC2.peptide.svr19 8.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
878
103
479
292.7
33.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.036 ± 0.936
2.506 ± 0.407
4.897 ± 2.67
7.062 ± 2.046
5.353 ± 0.818
5.695 ± 0.297
1.822 ± 1.345
5.695 ± 1.475
6.72 ± 0.588
7.745 ± 1.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.05 ± 0.508
5.353 ± 0.905
4.556 ± 0.701
4.784 ± 0.816
6.264 ± 0.384
6.72 ± 1.057
5.467 ± 0.527
5.923 ± 1.351
1.253 ± 0.318
4.1 ± 0.656
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here