Aglaonema bacilliform virus
Average proteome isoelectric point is 7.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411F6N3|A0A411F6N3_9VIRU ORF1 OS=Aglaonema bacilliform virus OX=1512278 PE=4 SV=1
MM1 pKa = 7.56 TSQPSTSQITTAPLFEE17 pKa = 4.58 DD18 pKa = 3.58 QIRR21 pKa = 11.84 DD22 pKa = 3.28 YY23 pKa = 11.14 RR24 pKa = 11.84 RR25 pKa = 11.84 NQRR28 pKa = 11.84 RR29 pKa = 11.84 LYY31 pKa = 9.96 NARR34 pKa = 11.84 QAVRR38 pKa = 11.84 RR39 pKa = 11.84 ISRR42 pKa = 11.84 NITGAHH48 pKa = 4.61 TPYY51 pKa = 10.55 RR52 pKa = 11.84 VTLEE56 pKa = 4.0 QQIDD60 pKa = 3.66 PQAQLRR66 pKa = 11.84 MSMQEE71 pKa = 3.6 RR72 pKa = 11.84 ASIVPAEE79 pKa = 4.21 VLYY82 pKa = 10.7 HH83 pKa = 6.45 SRR85 pKa = 11.84 TDD87 pKa = 4.44 DD88 pKa = 3.19 IHH90 pKa = 7.59 HH91 pKa = 7.2 KK92 pKa = 10.11 IYY94 pKa = 10.27 VHH96 pKa = 6.77 RR97 pKa = 11.84 SEE99 pKa = 5.53 EE100 pKa = 4.09 VILCIKK106 pKa = 10.31 QVDD109 pKa = 3.59 RR110 pKa = 11.84 VFIQEE115 pKa = 4.0 EE116 pKa = 4.72 SFQQLQRR123 pKa = 11.84 SRR125 pKa = 11.84 MAFIHH130 pKa = 6.32 IGVIQVRR137 pKa = 11.84 LQILHH142 pKa = 6.19 RR143 pKa = 11.84 QHH145 pKa = 6.92 EE146 pKa = 4.8 GTMALVVFRR155 pKa = 11.84 DD156 pKa = 3.58 NRR158 pKa = 11.84 WPDD161 pKa = 3.23 DD162 pKa = 3.89 RR163 pKa = 11.84 SIFATMEE170 pKa = 3.6 VDD172 pKa = 3.52 LTLGSQLVYY181 pKa = 10.24 VIPDD185 pKa = 3.22 TMMTIGDD192 pKa = 4.67 FYY194 pKa = 11.57 RR195 pKa = 11.84 NIQISILTKK204 pKa = 10.44 GYY206 pKa = 10.22 EE207 pKa = 3.98 GWQGGEE213 pKa = 4.02 ANLLITRR220 pKa = 11.84 GLVGRR225 pKa = 11.84 LSNTSNVGFAYY236 pKa = 10.59 NIQNVVDD243 pKa = 3.84 YY244 pKa = 10.31 LVSHH248 pKa = 6.63 GVRR251 pKa = 11.84 ALPGQRR257 pKa = 11.84 YY258 pKa = 7.11 STQEE262 pKa = 3.8 LQGHH266 pKa = 5.66 NWILHH271 pKa = 5.27 PTTTHH276 pKa = 6.97 IPLQPQEE283 pKa = 4.21 VTSTNMLDD291 pKa = 3.34 GSIALRR297 pKa = 11.84 LSNYY301 pKa = 9.35 GPAPTAIIPHH311 pKa = 6.62 DD312 pKa = 4.07 EE313 pKa = 4.17 EE314 pKa = 4.27 VHH316 pKa = 7.05 SDD318 pKa = 3.58 EE319 pKa = 4.36 EE320 pKa = 3.98 QIIAVFCLDD329 pKa = 5.94 DD330 pKa = 4.48 YY331 pKa = 12.08 DD332 pKa = 5.87 DD333 pKa = 6.24 DD334 pKa = 4.96 NDD336 pKa = 4.46 EE337 pKa = 4.2 EE338 pKa = 4.93 GNYY341 pKa = 8.61 YY342 pKa = 9.85 WYY344 pKa = 10.73 YY345 pKa = 10.36 NSPVSPANGYY355 pKa = 9.76 IDD357 pKa = 5.46 LPYY360 pKa = 10.14 PDD362 pKa = 5.57 LPYY365 pKa = 9.94 PQQYY369 pKa = 9.94 YY370 pKa = 7.56 PTEE373 pKa = 4.47 RR374 pKa = 11.84 PASPIPFQAEE384 pKa = 3.82 PSNITDD390 pKa = 3.96 IPPPPIFDD398 pKa = 4.38 EE399 pKa = 4.57 YY400 pKa = 10.7 PSDD403 pKa = 3.7 TEE405 pKa = 4.54 AVAEE409 pKa = 4.37 EE410 pKa = 4.11 YY411 pKa = 10.69 TIVRR415 pKa = 11.84 EE416 pKa = 3.99 MLPTIAASVEE426 pKa = 4.56 EE427 pKa = 4.29 YY428 pKa = 10.4 PQMKK432 pKa = 9.76 QLEE435 pKa = 4.34 EE436 pKa = 4.16 LLASSSAISSYY447 pKa = 10.57 RR448 pKa = 11.84 PPEE451 pKa = 4.21 DD452 pKa = 3.0 TVMGPPTYY460 pKa = 10.65 GPASQEE466 pKa = 4.14 SLKK469 pKa = 11.07 SKK471 pKa = 10.42 ASSSRR476 pKa = 11.84 PQYY479 pKa = 9.76 EE480 pKa = 4.26 GSSSRR485 pKa = 11.84 TRR487 pKa = 11.84 TTFKK491 pKa = 10.62 TKK493 pKa = 10.29 DD494 pKa = 3.32 YY495 pKa = 10.94 SEE497 pKa = 3.97 WWNLPSAQHH506 pKa = 4.82 NTGAIFTIPTQLGMFNDD523 pKa = 3.58 VFLRR527 pKa = 11.84 WEE529 pKa = 4.76 SITKK533 pKa = 10.28 NLVSLQGFTNSQDD546 pKa = 2.88 KK547 pKa = 11.46 VEE549 pKa = 5.19 FIEE552 pKa = 4.25 NLLGEE557 pKa = 4.43 AEE559 pKa = 4.27 KK560 pKa = 9.92 LTWIQWRR567 pKa = 11.84 MSYY570 pKa = 8.19 PTEE573 pKa = 3.99 YY574 pKa = 10.81 QLLLDD579 pKa = 4.44 SADD582 pKa = 3.48 GRR584 pKa = 11.84 QGTQNILSQLRR595 pKa = 11.84 RR596 pKa = 11.84 IFILEE601 pKa = 4.26 DD602 pKa = 3.38 PFQGSTALQEE612 pKa = 3.76 DD613 pKa = 5.24 AYY615 pKa = 11.48 RR616 pKa = 11.84 NIEE619 pKa = 4.33 KK620 pKa = 10.63 LSCTNIKK627 pKa = 10.56 DD628 pKa = 3.21 ILPYY632 pKa = 10.44 LNEE635 pKa = 3.96 YY636 pKa = 8.66 MHH638 pKa = 7.15 LAAKK642 pKa = 8.45 TGRR645 pKa = 11.84 MYY647 pKa = 10.97 INTEE651 pKa = 3.75 LSEE654 pKa = 4.58 KK655 pKa = 9.95 IWTKK659 pKa = 10.32 MPGDD663 pKa = 4.42 LGQKK667 pKa = 9.62 IKK669 pKa = 11.16 AEE671 pKa = 4.13 YY672 pKa = 7.79 EE673 pKa = 3.66 AKK675 pKa = 10.9 YY676 pKa = 9.66 PGNTIGVIPRR686 pKa = 11.84 ILFSYY691 pKa = 10.22 KK692 pKa = 10.28 YY693 pKa = 10.58 LEE695 pKa = 4.28 NEE697 pKa = 4.04 CRR699 pKa = 11.84 DD700 pKa = 3.58 AAFKK704 pKa = 10.77 RR705 pKa = 11.84 SLKK708 pKa = 10.62 GLSFCKK714 pKa = 10.25 DD715 pKa = 2.96 IPIPGYY721 pKa = 8.2 YY722 pKa = 8.92 QSKK725 pKa = 9.19 RR726 pKa = 11.84 KK727 pKa = 10.15 YY728 pKa = 9.78 GIRR731 pKa = 11.84 KK732 pKa = 6.57 ATTYY736 pKa = 10.13 KK737 pKa = 10.56 GKK739 pKa = 9.02 PHH741 pKa = 6.72 NSHH744 pKa = 6.42 ARR746 pKa = 11.84 IEE748 pKa = 4.33 KK749 pKa = 10.06 RR750 pKa = 11.84 KK751 pKa = 9.69 HH752 pKa = 3.53 LTRR755 pKa = 11.84 NKK757 pKa = 9.82 RR758 pKa = 11.84 CKK760 pKa = 10.26 CYY762 pKa = 10.71 LCGQDD767 pKa = 2.63 GHH769 pKa = 6.1 YY770 pKa = 10.9 ARR772 pKa = 11.84 EE773 pKa = 4.11 CPNDD777 pKa = 3.45 KK778 pKa = 10.81 KK779 pKa = 10.77 SVKK782 pKa = 9.79 RR783 pKa = 11.84 VAVFEE788 pKa = 4.29 NLDD791 pKa = 3.81 LPDD794 pKa = 5.24 DD795 pKa = 4.18 YY796 pKa = 11.55 DD797 pKa = 3.46 IVSVQEE803 pKa = 4.27 GEE805 pKa = 4.17 EE806 pKa = 4.08 QSDD809 pKa = 4.01 SIYY812 pKa = 10.71 SISEE816 pKa = 3.98 EE817 pKa = 3.93 EE818 pKa = 4.53 DD819 pKa = 3.69 GNIHH823 pKa = 6.47 EE824 pKa = 5.42 LLNKK828 pKa = 10.04 LHH830 pKa = 6.03 ITEE833 pKa = 4.36 HH834 pKa = 6.22 ALVFRR839 pKa = 11.84 EE840 pKa = 3.91 EE841 pKa = 4.18 DD842 pKa = 2.93 HH843 pKa = 6.94 TYY845 pKa = 10.29 WIGQPDD851 pKa = 5.21 GYY853 pKa = 10.98 RR854 pKa = 11.84 SMVKK858 pKa = 8.61 VTQAQHH864 pKa = 6.96 DD865 pKa = 4.3 CQHH868 pKa = 6.46 DD869 pKa = 3.7 WEE871 pKa = 4.99 FNSPNPSLCLCCKK884 pKa = 10.12 IEE886 pKa = 4.16 TSVNTRR892 pKa = 11.84 MSCKK896 pKa = 9.7 KK897 pKa = 9.73 CTITVCNLCAYY908 pKa = 10.16 HH909 pKa = 6.49 YY910 pKa = 8.16 FQQKK914 pKa = 7.56 VHH916 pKa = 6.84 ILRR919 pKa = 11.84 PDD921 pKa = 3.78 TIKK924 pKa = 10.71 FNQRR928 pKa = 11.84 PLLRR932 pKa = 11.84 EE933 pKa = 3.49 QHH935 pKa = 6.65 EE936 pKa = 4.32 YY937 pKa = 10.39 IIWCEE942 pKa = 3.72 AEE944 pKa = 4.01 IQRR947 pKa = 11.84 LQHH950 pKa = 6.06 EE951 pKa = 5.04 LNTAQMQADD960 pKa = 3.93 YY961 pKa = 10.04 WHH963 pKa = 6.66 NKK965 pKa = 8.97 YY966 pKa = 10.84 LDD968 pKa = 3.57 TQAPLLQQTYY978 pKa = 10.09 EE979 pKa = 4.2 EE980 pKa = 5.15 LATDD984 pKa = 4.35 PKK986 pKa = 10.76 GKK988 pKa = 10.17 RR989 pKa = 11.84 PMEE992 pKa = 4.37 EE993 pKa = 3.76 EE994 pKa = 4.01 THH996 pKa = 6.48 VFTQVSSPQVSSRR1009 pKa = 11.84 KK1010 pKa = 9.19 NLLYY1014 pKa = 10.86 NLIVFLDD1021 pKa = 3.43 IPGNEE1026 pKa = 4.17 RR1027 pKa = 11.84 ISLRR1031 pKa = 11.84 AILDD1035 pKa = 3.59 TGATTCCVDD1044 pKa = 3.43 INSIPPEE1051 pKa = 4.35 AIEE1054 pKa = 3.83 EE1055 pKa = 4.15 NPYY1058 pKa = 9.97 VVHH1061 pKa = 7.08 FSGINSKK1068 pKa = 7.41 TTANKK1073 pKa = 8.53 KK1074 pKa = 9.67 LKK1076 pKa = 9.06 YY1077 pKa = 10.39 GRR1079 pKa = 11.84 MLIQEE1084 pKa = 3.89 NSFRR1088 pKa = 11.84 IPYY1091 pKa = 9.2 TYY1093 pKa = 10.72 AFPMNLGEE1101 pKa = 4.75 SIQLILGCNFIRR1113 pKa = 11.84 AMQGGVRR1120 pKa = 11.84 IEE1122 pKa = 4.02 GDD1124 pKa = 3.35 VIVFYY1129 pKa = 11.46 KK1130 pKa = 10.82 NITQINTQPSPSSCAIEE1147 pKa = 4.1 LLEE1150 pKa = 6.18 GEE1152 pKa = 4.6 DD1153 pKa = 3.79 QLIEE1157 pKa = 4.09 PEE1159 pKa = 3.96 FDD1161 pKa = 4.51 FEE1163 pKa = 5.03 VPCCVASSFHH1173 pKa = 7.58 NMDD1176 pKa = 4.8 LLDD1179 pKa = 3.75 EE1180 pKa = 4.8 LKK1182 pKa = 10.28 RR1183 pKa = 11.84 AGYY1186 pKa = 10.15 VGEE1189 pKa = 5.16 DD1190 pKa = 3.72 PLKK1193 pKa = 9.89 FWASNKK1199 pKa = 9.44 IMCHH1203 pKa = 6.42 LDD1205 pKa = 2.99 IKK1207 pKa = 11.21 NPDD1210 pKa = 4.22 LIIEE1214 pKa = 4.49 DD1215 pKa = 4.31 RR1216 pKa = 11.84 PLKK1219 pKa = 10.36 QISPTLEE1226 pKa = 3.49 AAYY1229 pKa = 9.51 KK1230 pKa = 10.04 KK1231 pKa = 10.33 HH1232 pKa = 6.56 IDD1234 pKa = 3.42 ALLALHH1240 pKa = 6.87 VIRR1243 pKa = 11.84 PSTSRR1248 pKa = 11.84 HH1249 pKa = 3.56 RR1250 pKa = 11.84 TAAIIVNSGISIDD1263 pKa = 3.84 PVTQQEE1269 pKa = 4.38 VRR1271 pKa = 11.84 GKK1273 pKa = 9.92 EE1274 pKa = 3.57 RR1275 pKa = 11.84 LVFNYY1280 pKa = 9.57 KK1281 pKa = 10.05 RR1282 pKa = 11.84 LNDD1285 pKa = 3.77 NTHH1288 pKa = 6.57 KK1289 pKa = 10.71 DD1290 pKa = 3.55 QYY1292 pKa = 10.72 SLPGINTIMQRR1303 pKa = 11.84 IGHH1306 pKa = 5.45 SKK1308 pKa = 9.82 IYY1310 pKa = 10.8 SKK1312 pKa = 11.19 FDD1314 pKa = 3.64 LKK1316 pKa = 11.29 SGFHH1320 pKa = 6.33 QIAMHH1325 pKa = 7.03 PDD1327 pKa = 3.6 SIEE1330 pKa = 3.56 WTAFWTPQGLFEE1342 pKa = 4.41 WLVMPFGLKK1351 pKa = 9.44 NAPSIFQRR1359 pKa = 11.84 KK1360 pKa = 7.34 MDD1362 pKa = 3.68 NCFKK1366 pKa = 10.24 DD1367 pKa = 3.47 TEE1369 pKa = 4.38 GFIAVYY1375 pKa = 10.08 IDD1377 pKa = 4.98 DD1378 pKa = 4.3 ILVFSQNEE1386 pKa = 4.27 TEE1388 pKa = 4.38 HH1389 pKa = 6.39 EE1390 pKa = 3.93 KK1391 pKa = 10.72 HH1392 pKa = 6.07 LRR1394 pKa = 11.84 IMLNICRR1401 pKa = 11.84 KK1402 pKa = 9.68 HH1403 pKa = 7.07 GLILSPSKK1411 pKa = 10.48 MKK1413 pKa = 10.33 IGVPIIHH1420 pKa = 6.75 FLGATIGNQKK1430 pKa = 9.35 IQLQEE1435 pKa = 4.38 HH1436 pKa = 5.74 IVKK1439 pKa = 10.57 KK1440 pKa = 10.15 ILHH1443 pKa = 6.23 YY1444 pKa = 10.49 DD1445 pKa = 3.67 DD1446 pKa = 5.52 KK1447 pKa = 11.78 EE1448 pKa = 4.37 LMTKK1452 pKa = 10.14 KK1453 pKa = 10.45 GLRR1456 pKa = 11.84 SWLGILNYY1464 pKa = 9.82 ARR1466 pKa = 11.84 NYY1468 pKa = 9.58 IPHH1471 pKa = 7.17 LGKK1474 pKa = 9.04 LTGPLYY1480 pKa = 11.01 AKK1482 pKa = 9.25 TSPTGEE1488 pKa = 4.04 KK1489 pKa = 10.77 AMNQQDD1495 pKa = 3.69 WDD1497 pKa = 3.93 CVRR1500 pKa = 11.84 KK1501 pKa = 9.92 IKK1503 pKa = 10.56 EE1504 pKa = 4.07 IVKK1507 pKa = 9.57 QLPDD1511 pKa = 4.07 LDD1513 pKa = 4.46 IPPPSCFIIIEE1524 pKa = 4.28 TDD1526 pKa = 2.87 GCMSGWGGICKK1537 pKa = 9.72 WKK1539 pKa = 9.53 NQEE1542 pKa = 3.74 YY1543 pKa = 10.99 DD1544 pKa = 3.94 PISSEE1549 pKa = 4.35 KK1550 pKa = 9.39 ICAYY1554 pKa = 10.52 ASGKK1558 pKa = 9.81 FDD1560 pKa = 4.16 PPKK1563 pKa = 9.97 STIDD1567 pKa = 3.68 AEE1569 pKa = 4.05 IHH1571 pKa = 6.12 AVMNSLDD1578 pKa = 3.81 KK1579 pKa = 11.21 FKK1581 pKa = 10.94 LYY1583 pKa = 11.09 YY1584 pKa = 10.03 LDD1586 pKa = 4.8 KK1587 pKa = 10.51 KK1588 pKa = 10.99 DD1589 pKa = 4.43 LIIRR1593 pKa = 11.84 TDD1595 pKa = 3.49 CQAIISFYY1603 pKa = 11.17 NKK1605 pKa = 9.77 SAQNKK1610 pKa = 7.29 PSRR1613 pKa = 11.84 VRR1615 pKa = 11.84 WISFTDD1621 pKa = 4.96 FITGTGITIKK1631 pKa = 9.99 FQHH1634 pKa = 6.62 ISGSDD1639 pKa = 3.13 NKK1641 pKa = 10.78 LADD1644 pKa = 3.65 TLSRR1648 pKa = 11.84 LINVLIIQPEE1658 pKa = 4.47 SIPLNISEE1666 pKa = 5.12 GIEE1669 pKa = 3.91 KK1670 pKa = 10.45 ALEE1673 pKa = 4.07 EE1674 pKa = 4.38 VTQRR1678 pKa = 11.84 PQVAQVRR1685 pKa = 11.84 KK1686 pKa = 8.06 LTHH1689 pKa = 6.97 LISSFLTDD1697 pKa = 2.96 SHH1699 pKa = 7.42 KK1700 pKa = 10.89 VEE1702 pKa = 4.01 RR1703 pKa = 11.84 LFVITEE1709 pKa = 3.75 EE1710 pKa = 4.26 STYY1713 pKa = 11.22 SEE1715 pKa = 4.13 ILHH1718 pKa = 6.65 EE1719 pKa = 4.26 LTQLPMPGDD1728 pKa = 4.19 RR1729 pKa = 11.84 YY1730 pKa = 10.67 QDD1732 pKa = 3.54 LTRR1735 pKa = 11.84 LASRR1739 pKa = 11.84 SRR1741 pKa = 11.84 EE1742 pKa = 3.81 NTQRR1746 pKa = 11.84 ALFLALDD1753 pKa = 4.09 DD1754 pKa = 3.44 VWRR1757 pKa = 11.84 NIQQQVHH1764 pKa = 5.9 FFHH1767 pKa = 7.87 RR1768 pKa = 11.84 IATRR1772 pKa = 11.84 DD1773 pKa = 3.35 NYY1775 pKa = 11.31 YY1776 pKa = 11.03 GDD1778 pKa = 4.91 HH1779 pKa = 6.95 LPQLLQKK1786 pKa = 9.88 QDD1788 pKa = 3.47 RR1789 pKa = 11.84 FLHH1792 pKa = 6.38 LSQKK1796 pKa = 8.78 MRR1798 pKa = 11.84 HH1799 pKa = 5.87 CLRR1802 pKa = 11.84 NSPDD1806 pKa = 3.19 PP1807 pKa = 4.02
Molecular weight: 207.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.905
IPC2_protein 5.893
IPC_protein 5.97
Toseland 6.351
ProMoST 6.351
Dawson 6.275
Bjellqvist 6.275
Wikipedia 6.287
Rodwell 6.262
Grimsley 6.504
Solomon 6.275
Lehninger 6.262
Nozaki 6.561
DTASelect 6.737
Thurlkill 6.751
EMBOSS 6.722
Sillero 6.649
Patrickios 4.012
IPC_peptide 6.287
IPC2_peptide 6.605
IPC2.peptide.svr19 6.571
Protein with the highest isoelectric point:
>tr|A0A411F6T8|A0A411F6T8_9VIRU ORF2 OS=Aglaonema bacilliform virus OX=1512278 PE=4 SV=1
MM1 pKa = 7.91 SYY3 pKa = 11.0 INTQASTAYY12 pKa = 10.12 QEE14 pKa = 5.75 AITATNAIEE23 pKa = 3.95 EE24 pKa = 4.27 PAIGFAKK31 pKa = 10.32 PADD34 pKa = 3.64 FTGAQLGTKK43 pKa = 9.98 CVIKK47 pKa = 10.7 QNNLQIEE54 pKa = 4.67 LLAKK58 pKa = 10.11 ILSEE62 pKa = 4.01 VTKK65 pKa = 10.58 VKK67 pKa = 10.44 EE68 pKa = 3.73 VLEE71 pKa = 4.15 QIKK74 pKa = 10.28 KK75 pKa = 9.85 EE76 pKa = 3.96 KK77 pKa = 10.57 AKK79 pKa = 10.3 EE80 pKa = 3.94 IPEE83 pKa = 4.07 DD84 pKa = 4.73 LITGLKK90 pKa = 9.24 NLKK93 pKa = 10.1 LGPTKK98 pKa = 10.38 EE99 pKa = 3.96 KK100 pKa = 10.82 SGLLRR105 pKa = 11.84 VYY107 pKa = 10.68 KK108 pKa = 10.51 NPKK111 pKa = 8.13 TLLEE115 pKa = 4.27 AEE117 pKa = 4.49 KK118 pKa = 10.86 SKK120 pKa = 10.93 QQ121 pKa = 3.21
Molecular weight: 13.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.639
IPC2_protein 8.404
IPC_protein 8.346
Toseland 9.311
ProMoST 8.873
Dawson 9.472
Bjellqvist 9.121
Wikipedia 9.575
Rodwell 10.028
Grimsley 9.487
Solomon 9.575
Lehninger 9.56
Nozaki 9.355
DTASelect 9.092
Thurlkill 9.341
EMBOSS 9.692
Sillero 9.414
Patrickios 7.658
IPC_peptide 9.575
IPC2_peptide 7.571
IPC2.peptide.svr19 7.832
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2052
121
1807
684.0
78.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.019 ± 1.413
1.754 ± 0.456
5.214 ± 1.742
7.31 ± 2.515
3.265 ± 0.593
4.483 ± 1.128
3.021 ± 0.917
8.333 ± 1.114
6.579 ± 3.52
9.211 ± 2.245
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.949 ± 0.551
4.63 ± 0.129
5.312 ± 1.319
5.945 ± 0.151
5.214 ± 1.257
6.969 ± 1.122
5.848 ± 0.717
4.386 ± 0.137
1.365 ± 0.582
4.191 ± 0.848
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here