Xylella phage Paz
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5Q9M1|V5Q9M1_9CAUD Internal virion protein OS=Xylella phage Paz OX=1415145 GN=Paz_38 PE=4 SV=1
MM1 pKa = 7.06 ATIAAKK7 pKa = 10.63 LPFAPGTTVQLSAAVDD23 pKa = 3.91 VYY25 pKa = 10.96 PDD27 pKa = 3.33 KK28 pKa = 11.43 APAYY32 pKa = 8.22 VCFYY36 pKa = 10.55 QIVGTTLYY44 pKa = 10.49 VQVRR48 pKa = 11.84 VQDD51 pKa = 4.0 DD52 pKa = 3.89 SPEE55 pKa = 4.02 VGTFDD60 pKa = 5.09 LFIPVTGIEE69 pKa = 4.17 ALGTPP74 pKa = 4.47
Molecular weight: 7.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.85
IPC2_protein 4.101
IPC_protein 3.91
Toseland 3.706
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.846
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.037
Patrickios 0.604
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|V5Q7Q0|V5Q7Q0_9CAUD 3'-5' exonuclease OS=Xylella phage Paz OX=1415145 GN=Paz_21 PE=4 SV=1
MM1 pKa = 7.52 NYY3 pKa = 10.21 EE4 pKa = 4.19 NLTQARR10 pKa = 11.84 HH11 pKa = 3.88 QAEE14 pKa = 3.92 RR15 pKa = 11.84 VLTHH19 pKa = 5.97 ITLHH23 pKa = 5.67 RR24 pKa = 11.84 QAVRR28 pKa = 11.84 HH29 pKa = 5.53 GLVAKK34 pKa = 10.26 PDD36 pKa = 3.3 VSYY39 pKa = 11.02 RR40 pKa = 11.84 ALHH43 pKa = 6.05 EE44 pKa = 4.02 WAEE47 pKa = 4.21 SLKK50 pKa = 10.76 NIQSLVTVKK59 pKa = 10.19 RR60 pKa = 11.84 AARR63 pKa = 11.84 TTVKK67 pKa = 10.68 LLFNARR73 pKa = 11.84 RR74 pKa = 11.84 LWVGVHH80 pKa = 5.71 YY81 pKa = 11.05 SPANRR86 pKa = 11.84 RR87 pKa = 11.84 WCVNLLPGVTICITKK102 pKa = 10.54 AGGVEE107 pKa = 4.03 PP108 pKa = 4.81
Molecular weight: 12.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.809
IPC_protein 10.745
Toseland 10.818
ProMoST 10.599
Dawson 10.906
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.067
Grimsley 10.95
Solomon 11.067
Lehninger 11.023
Nozaki 10.804
DTASelect 10.657
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.847
Patrickios 10.818
IPC_peptide 11.067
IPC2_peptide 9.823
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
13480
56
1363
264.3
29.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.601 ± 0.529
0.727 ± 0.122
6.194 ± 0.277
5.816 ± 0.263
3.509 ± 0.123
7.864 ± 0.351
1.929 ± 0.165
4.792 ± 0.264
5.312 ± 0.309
7.938 ± 0.244
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.234 ± 0.279
4.184 ± 0.173
4.006 ± 0.23
4.369 ± 0.353
6.053 ± 0.244
5.497 ± 0.294
5.846 ± 0.241
6.84 ± 0.339
1.699 ± 0.097
3.591 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here