Caloranaerobacter azorensis H53214
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096CXZ2|A0A096CXZ2_9CLOT HAD family hydrolase OS=Caloranaerobacter azorensis H53214 OX=1156417 GN=Y919_00385 PE=4 SV=1
MM1 pKa = 8.26 DD2 pKa = 4.63 KK3 pKa = 11.36 NKK5 pKa = 10.28 IQVNEE10 pKa = 3.95 LNRR13 pKa = 11.84 LYY15 pKa = 11.12 DD16 pKa = 4.14 EE17 pKa = 5.34 IYY19 pKa = 10.47 YY20 pKa = 11.0 SNNNDD25 pKa = 2.93 VEE27 pKa = 4.3 QALEE31 pKa = 4.7 LISNLISEE39 pKa = 4.69 KK40 pKa = 10.3 EE41 pKa = 4.02 YY42 pKa = 10.62 EE43 pKa = 4.08 EE44 pKa = 5.01 SGIEE48 pKa = 4.23 VNDD51 pKa = 3.87 LSDD54 pKa = 4.27 IIHH57 pKa = 6.54 IAIEE61 pKa = 4.02 TLEE64 pKa = 4.53 EE65 pKa = 3.6 ISEE68 pKa = 4.24 IFDD71 pKa = 3.66 SEE73 pKa = 4.42 GLIDD77 pKa = 4.09 SYY79 pKa = 11.97 NFVIEE84 pKa = 4.47 KK85 pKa = 10.67 LEE87 pKa = 3.87 NLQNGEE93 pKa = 4.03 LDD95 pKa = 4.5 DD96 pKa = 5.52 IIEE99 pKa = 4.06 EE100 pKa = 4.42 EE101 pKa = 4.4 YY102 pKa = 11.52 NKK104 pKa = 10.22 FFDD107 pKa = 5.49 DD108 pKa = 5.6 DD109 pKa = 5.11 FIDD112 pKa = 4.15 EE113 pKa = 4.63 YY114 pKa = 11.52 GDD116 pKa = 3.44 EE117 pKa = 5.15 SEE119 pKa = 4.61 MEE121 pKa = 4.57 DD122 pKa = 3.8 YY123 pKa = 11.35
Molecular weight: 14.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.823
IPC2_protein 3.541
IPC_protein 3.503
Toseland 3.312
ProMoST 3.656
Dawson 3.49
Bjellqvist 3.643
Wikipedia 3.389
Rodwell 3.338
Grimsley 3.223
Solomon 3.465
Lehninger 3.414
Nozaki 3.605
DTASelect 3.757
Thurlkill 3.363
EMBOSS 3.401
Sillero 3.63
Patrickios 0.896
IPC_peptide 3.465
IPC2_peptide 3.605
IPC2.peptide.svr19 3.628
Protein with the highest isoelectric point:
>tr|A0A096BG55|A0A096BG55_9CLOT GTPase Era OS=Caloranaerobacter azorensis H53214 OX=1156417 GN=era PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.0 KK9 pKa = 7.87 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 8.39 KK14 pKa = 8.49 EE15 pKa = 3.5 HH16 pKa = 6.09 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MSTKK25 pKa = 10.09 SGRR28 pKa = 11.84 NVLKK32 pKa = 10.19 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.91 GRR39 pKa = 11.84 KK40 pKa = 9.02 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.36
IPC2_protein 10.921
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.501
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2148
0
2148
640499
37
2586
298.2
33.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.36 ± 0.054
0.942 ± 0.019
5.568 ± 0.041
7.709 ± 0.068
4.253 ± 0.043
6.408 ± 0.053
1.337 ± 0.02
10.981 ± 0.068
9.52 ± 0.065
9.237 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.453 ± 0.026
5.798 ± 0.046
2.949 ± 0.033
2.217 ± 0.028
3.984 ± 0.039
5.451 ± 0.035
4.522 ± 0.045
6.614 ± 0.048
0.62 ± 0.016
4.077 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here