Drosophila obscura sigmavirus 10A
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C8CJE8|C8CJE8_9RHAB Uncharacterized protein X OS=Drosophila obscura sigmavirus 10A OX=666962 GN=X PE=4 SV=1
MM1 pKa = 7.03 STNPSKK7 pKa = 10.86 PMNRR11 pKa = 11.84 NSSEE15 pKa = 3.49 KK16 pKa = 10.29 DD17 pKa = 2.84 IRR19 pKa = 11.84 EE20 pKa = 4.0 NSSSDD25 pKa = 3.26 EE26 pKa = 4.08 FEE28 pKa = 4.57 EE29 pKa = 4.3 EE30 pKa = 4.43 VEE32 pKa = 4.23 EE33 pKa = 4.38 STSDD37 pKa = 3.49 TEE39 pKa = 5.3 KK40 pKa = 11.03 NIPDD44 pKa = 5.43 DD45 pKa = 3.75 ISSPDD50 pKa = 3.57 NEE52 pKa = 4.72 FVPAPNQNIKK62 pKa = 10.59 FSDD65 pKa = 3.42 AFNSEE70 pKa = 4.13 FGGKK74 pKa = 9.55 YY75 pKa = 10.08 NPEE78 pKa = 3.93 EE79 pKa = 3.87 LSKK82 pKa = 11.54 YY83 pKa = 10.19 GATDD87 pKa = 4.06 DD88 pKa = 3.96 YY89 pKa = 11.22 CVAGRR94 pKa = 11.84 LVQEE98 pKa = 4.24 KK99 pKa = 10.93 LNIKK103 pKa = 8.37 EE104 pKa = 4.03 TDD106 pKa = 3.18 EE107 pKa = 5.25 DD108 pKa = 3.84 NVEE111 pKa = 4.0 RR112 pKa = 11.84 AILEE116 pKa = 4.13 SSGVVPEE123 pKa = 5.08 AEE125 pKa = 3.98 AEE127 pKa = 4.06 MEE129 pKa = 4.16 EE130 pKa = 4.35 LCEE133 pKa = 4.5 KK134 pKa = 10.95 SSDD137 pKa = 3.7 EE138 pKa = 3.99 NLEE141 pKa = 4.18 CGATITLDD149 pKa = 4.12 DD150 pKa = 5.08 GFHH153 pKa = 6.36 KK154 pKa = 10.31 MKK156 pKa = 10.37 IIASLEE162 pKa = 3.82 EE163 pKa = 4.22 RR164 pKa = 11.84 FFDD167 pKa = 3.39 PTQIHH172 pKa = 7.14 LINRR176 pKa = 11.84 FFRR179 pKa = 11.84 EE180 pKa = 3.27 LALYY184 pKa = 10.82 VNTDD188 pKa = 3.26 EE189 pKa = 4.63 TEE191 pKa = 4.12 GPILDD196 pKa = 3.53 KK197 pKa = 11.12 SKK199 pKa = 10.93 NRR201 pKa = 11.84 GAIVFGYY208 pKa = 10.37 VEE210 pKa = 4.2 SKK212 pKa = 9.8 YY213 pKa = 10.54 QKK215 pKa = 8.42 KK216 pKa = 10.02 QKK218 pKa = 7.61 MTKK221 pKa = 10.18 QNDD224 pKa = 3.5 DD225 pKa = 4.68 NIPSTSRR232 pKa = 11.84 NCNKK236 pKa = 9.82 NVCQPKK242 pKa = 9.89 SPPPRR247 pKa = 11.84 SEE249 pKa = 3.94 GVAPAVNPNLQVILKK264 pKa = 9.76 DD265 pKa = 3.73 LNKK268 pKa = 9.97 GLKK271 pKa = 8.03 WKK273 pKa = 10.51 HH274 pKa = 6.25 FITQKK279 pKa = 10.35 EE280 pKa = 4.3 LSFKK284 pKa = 10.05 MGQTGMTVEE293 pKa = 4.78 EE294 pKa = 4.13 ISAVLEE300 pKa = 4.23 AGNEE304 pKa = 3.91 IKK306 pKa = 10.52 SGKK309 pKa = 10.17 EE310 pKa = 3.33 YY311 pKa = 11.15 VEE313 pKa = 3.92 YY314 pKa = 10.54 LFKK317 pKa = 10.72 IRR319 pKa = 11.84 GHH321 pKa = 6.14 FNQLEE326 pKa = 3.89 ASYY329 pKa = 10.24 IYY331 pKa = 10.51 QPP333 pKa = 3.7
Molecular weight: 37.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.781
IPC2_protein 4.749
IPC_protein 4.673
Toseland 4.533
ProMoST 4.749
Dawson 4.596
Bjellqvist 4.749
Wikipedia 4.431
Rodwell 4.507
Grimsley 4.444
Solomon 4.596
Lehninger 4.546
Nozaki 4.698
DTASelect 4.813
Thurlkill 4.52
EMBOSS 4.457
Sillero 4.774
Patrickios 4.062
IPC_peptide 4.609
IPC2_peptide 4.774
IPC2.peptide.svr19 4.73
Protein with the highest isoelectric point:
>tr|C8CJF0|C8CJF0_9RHAB Glycoprotein OS=Drosophila obscura sigmavirus 10A OX=666962 GN=G PE=4 SV=1
MM1 pKa = 7.37 SKK3 pKa = 10.57 KK4 pKa = 9.96 EE5 pKa = 4.61 AIPSLALMSTCIEE18 pKa = 4.09 GKK20 pKa = 10.09 EE21 pKa = 4.12 EE22 pKa = 4.12 IASSPHH28 pKa = 6.08 SSFFVPTAPVNDD40 pKa = 4.47 SIPYY44 pKa = 8.08 YY45 pKa = 9.88 VHH47 pKa = 6.69 NLTVEE52 pKa = 4.13 AEE54 pKa = 3.87 ISVNSNRR61 pKa = 11.84 VIDD64 pKa = 3.43 TWEE67 pKa = 4.0 PLRR70 pKa = 11.84 EE71 pKa = 4.02 ILTSWVDD78 pKa = 3.44 NYY80 pKa = 11.02 KK81 pKa = 11.0 GPLNVKK87 pKa = 9.99 SILGVVYY94 pKa = 9.62 TLLIFKK100 pKa = 8.2 MKK102 pKa = 10.11 KK103 pKa = 8.3 MRR105 pKa = 11.84 VGVNYY110 pKa = 10.5 LMYY113 pKa = 10.17 GIKK116 pKa = 9.56 MFDD119 pKa = 3.57 RR120 pKa = 11.84 ISFLMTYY127 pKa = 9.82 NFEE130 pKa = 4.03 NSIPVQGEE138 pKa = 3.94 LNYY141 pKa = 10.7 KK142 pKa = 9.66 SGRR145 pKa = 11.84 GQFLYY150 pKa = 10.13 TVNFKK155 pKa = 10.93 YY156 pKa = 10.47 RR157 pKa = 11.84 LTPIKK162 pKa = 10.34 RR163 pKa = 11.84 HH164 pKa = 5.37 GSLIQTLFSPGVVDD178 pKa = 3.32 MSKK181 pKa = 10.4 QVGPTLAVALDD192 pKa = 3.93 SIGIKK197 pKa = 9.36 TKK199 pKa = 10.34 EE200 pKa = 4.09 DD201 pKa = 3.49 PAGLVLLYY209 pKa = 7.51 TTT211 pKa = 4.93
Molecular weight: 23.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.455
IPC2_protein 8.463
IPC_protein 8.434
Toseland 8.946
ProMoST 8.799
Dawson 9.253
Bjellqvist 9.019
Wikipedia 9.443
Rodwell 9.399
Grimsley 9.326
Solomon 9.355
Lehninger 9.311
Nozaki 9.004
DTASelect 8.99
Thurlkill 9.077
EMBOSS 9.37
Sillero 9.209
Patrickios 4.342
IPC_peptide 9.341
IPC2_peptide 7.644
IPC2.peptide.svr19 7.651
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4052
211
2146
675.3
77.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.763 ± 0.769
1.234 ± 0.159
5.01 ± 0.198
6.318 ± 0.881
4.22 ± 0.175
5.503 ± 0.353
2.468 ± 0.356
7.28 ± 0.481
6.885 ± 0.338
10.242 ± 0.692
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.295 ± 0.324
5.528 ± 0.378
4.541 ± 0.34
3.258 ± 0.297
4.837 ± 0.518
8.119 ± 0.365
6.145 ± 0.29
5.602 ± 0.483
1.9 ± 0.255
3.85 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here