Penicillium arizonense
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1F5LKF4|A0A1F5LKF4_9EURO MFS domain-containing protein OS=Penicillium arizonense OX=1835702 GN=PENARI_c007G11189 PE=4 SV=1
MM1 pKa = 7.06 TFNVFMTGLLAATAVALPQPHH22 pKa = 5.68 WTRR25 pKa = 11.84 DD26 pKa = 3.29 TEE28 pKa = 4.92 SNVEE32 pKa = 3.88 ARR34 pKa = 11.84 SSGGGVQIVNNMDD47 pKa = 3.46 TNVYY51 pKa = 9.66 LWSTDD56 pKa = 3.24 SGPGSMQTISSGGGDD71 pKa = 4.11 YY72 pKa = 10.91 SEE74 pKa = 4.51 KK75 pKa = 10.52 WNTNSNGGGISIKK88 pKa = 10.19 MSTTEE93 pKa = 4.06 SEE95 pKa = 4.4 SSVLQFEE102 pKa = 4.59 YY103 pKa = 10.13 TQDD106 pKa = 2.86 GSTIYY111 pKa = 10.46 WDD113 pKa = 3.58 MSSIDD118 pKa = 4.66 LDD120 pKa = 3.84 STSDD124 pKa = 3.67 FVKK127 pKa = 10.64 SGFTVQPSDD136 pKa = 3.66 SSCTSVSCAAGDD148 pKa = 4.13 ANCKK152 pKa = 8.72 EE153 pKa = 4.92 AYY155 pKa = 9.02 QKK157 pKa = 10.99 PDD159 pKa = 3.39 DD160 pKa = 4.46 VNTNSCSLDD169 pKa = 3.23 AGFTLTLGG177 pKa = 3.67
Molecular weight: 18.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.605
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A1F5L377|A0A1F5L377_9EURO Uncharacterized protein OS=Penicillium arizonense OX=1835702 GN=PENARI_c044G00615 PE=4 SV=1
MM1 pKa = 7.62 ARR3 pKa = 11.84 RR4 pKa = 11.84 PIRR7 pKa = 11.84 IQLRR11 pKa = 11.84 RR12 pKa = 11.84 AGRR15 pKa = 11.84 NRR17 pKa = 11.84 RR18 pKa = 11.84 VPARR22 pKa = 11.84 ILTT25 pKa = 3.81
Molecular weight: 3.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12121
0
12121
5759560
8
6183
475.2
52.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.523 ± 0.019
1.233 ± 0.007
5.647 ± 0.016
6.06 ± 0.023
3.835 ± 0.014
6.864 ± 0.02
2.413 ± 0.01
5.097 ± 0.013
4.602 ± 0.02
9.02 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.251 ± 0.009
3.772 ± 0.012
5.957 ± 0.023
4.048 ± 0.014
5.868 ± 0.019
8.338 ± 0.023
5.993 ± 0.015
6.153 ± 0.015
1.501 ± 0.008
2.825 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here