Salmonella phage SEN34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Brunovirus; Salmonella virus SEN34

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4R365|A0A0M4R365_9CAUD Endolysin OS=Salmonella phage SEN34 OX=1647463 GN=SEN34_62 PE=3 SV=1
MM1 pKa = 7.42NIDD4 pKa = 3.47KK5 pKa = 10.71QALCIEE11 pKa = 4.24NAFDD15 pKa = 4.7IANQLYY21 pKa = 9.84EE22 pKa = 4.05LANNEE27 pKa = 4.0IEE29 pKa = 4.49CDD31 pKa = 3.61LFAVTSTNEE40 pKa = 3.66NGTEE44 pKa = 3.92IEE46 pKa = 4.22FEE48 pKa = 4.27RR49 pKa = 11.84PITDD53 pKa = 3.89LALEE57 pKa = 4.28ASSTINALLNRR68 pKa = 11.84LEE70 pKa = 4.08AAEE73 pKa = 4.04KK74 pKa = 10.32HH75 pKa = 5.17IEE77 pKa = 3.89AWGVKK82 pKa = 8.74YY83 pKa = 10.56IPLSGSSPVIPGGWISCSEE102 pKa = 4.29RR103 pKa = 11.84MPDD106 pKa = 2.9IQTDD110 pKa = 3.68VLIATVYY117 pKa = 11.34DD118 pKa = 3.74NVLQALLIADD128 pKa = 4.56RR129 pKa = 11.84FIEE132 pKa = 4.08PSGMDD137 pKa = 3.17FHH139 pKa = 8.59LDD141 pKa = 3.55EE142 pKa = 5.2VIGWMPLPDD151 pKa = 5.31APQQDD156 pKa = 3.97ILMKK160 pKa = 10.61SSSS163 pKa = 3.25

Molecular weight: 18.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3ULK6|A0A0M3ULK6_9CAUD DNA polymerase III theta subunit OS=Salmonella phage SEN34 OX=1647463 GN=SEN34_51 PE=4 SV=1
MM1 pKa = 7.34HH2 pKa = 7.16HH3 pKa = 7.18AKK5 pKa = 10.57LPTLKK10 pKa = 10.36YY11 pKa = 10.31VRR13 pKa = 11.84EE14 pKa = 3.97LLDD17 pKa = 3.79YY18 pKa = 10.35NPSSGVFTRR27 pKa = 11.84KK28 pKa = 9.08ISKK31 pKa = 9.76GGKK34 pKa = 8.93SVGSVAGSIDD44 pKa = 3.35RR45 pKa = 11.84DD46 pKa = 3.12GRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84ITLKK54 pKa = 9.49GVKK57 pKa = 9.6YY58 pKa = 10.2FSSRR62 pKa = 11.84IAWLFLTGQDD72 pKa = 4.11PGDD75 pKa = 3.95AEE77 pKa = 3.88IDD79 pKa = 3.61HH80 pKa = 6.79INRR83 pKa = 11.84NRR85 pKa = 11.84SDD87 pKa = 3.95DD88 pKa = 3.45SAANLRR94 pKa = 11.84ISTRR98 pKa = 11.84VQNARR103 pKa = 11.84NTGLRR108 pKa = 11.84SDD110 pKa = 3.79NQVGVQCVGRR120 pKa = 11.84IGKK123 pKa = 10.37GSGKK127 pKa = 8.04TLLLARR133 pKa = 11.84VVTGSVRR140 pKa = 11.84EE141 pKa = 4.07RR142 pKa = 11.84KK143 pKa = 7.66TFPDD147 pKa = 3.62TNEE150 pKa = 3.7GLQAARR156 pKa = 11.84KK157 pKa = 7.5WVLRR161 pKa = 11.84MKK163 pKa = 9.93EE164 pKa = 3.84KK165 pKa = 10.27HH166 pKa = 6.02HH167 pKa = 7.14SEE169 pKa = 4.19FSSMGAFNDD178 pKa = 3.6

Molecular weight: 19.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12305

42

669

195.3

21.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.134 ± 0.436

0.902 ± 0.136

6.054 ± 0.268

5.721 ± 0.312

3.446 ± 0.286

7.469 ± 0.327

1.463 ± 0.117

6.168 ± 0.357

5.697 ± 0.31

8.606 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.158

4.697 ± 0.191

4.023 ± 0.204

3.828 ± 0.212

5.973 ± 0.344

6.38 ± 0.235

6.51 ± 0.38

6.428 ± 0.274

1.707 ± 0.144

2.983 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski