Xanthomonas phage XAJ24
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I9L298|A0A1I9L298_9CAUD 5'-3' exonuclease OS=Xanthomonas phage XAJ24 OX=1775250 PE=4 SV=1
MM1 pKa = 6.64 VTMPLEE7 pKa = 4.47 EE8 pKa = 4.25 YY9 pKa = 9.91 TNLVAAKK16 pKa = 10.31 DD17 pKa = 3.87 EE18 pKa = 4.35 LDD20 pKa = 3.79 ALHH23 pKa = 7.13 AAGVDD28 pKa = 3.14 NWDD31 pKa = 3.79 FYY33 pKa = 12.1 YY34 pKa = 10.91 DD35 pKa = 3.64 ALRR38 pKa = 11.84 DD39 pKa = 3.74 AGLGDD44 pKa = 3.51 NDD46 pKa = 4.29 EE47 pKa = 4.42 EE48 pKa = 4.38 EE49 pKa = 4.83 EE50 pKa = 4.41 YY51 pKa = 11.42
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.826
IPC2_protein 3.592
IPC_protein 3.503
Toseland 3.325
ProMoST 3.706
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.478
Rodwell 3.35
Grimsley 3.236
Solomon 3.465
Lehninger 3.427
Nozaki 3.656
DTASelect 3.821
Thurlkill 3.401
EMBOSS 3.478
Sillero 3.643
Patrickios 0.604
IPC_peptide 3.465
IPC2_peptide 3.605
IPC2.peptide.svr19 3.645
Protein with the highest isoelectric point:
>tr|A0A1I9L282|A0A1I9L282_9CAUD Uncharacterized protein OS=Xanthomonas phage XAJ24 OX=1775250 PE=4 SV=1
MM1 pKa = 7.54 HH2 pKa = 7.41 GNANLEE8 pKa = 3.93 RR9 pKa = 11.84 TRR11 pKa = 11.84 TKK13 pKa = 10.69 RR14 pKa = 11.84 RR15 pKa = 11.84 FCQFTKK21 pKa = 9.13 TASVIDD27 pKa = 4.05 MTTTEE32 pKa = 4.03 YY33 pKa = 11.24 DD34 pKa = 3.1 RR35 pKa = 11.84 NLAEE39 pKa = 4.59 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 QAEE45 pKa = 4.14 RR46 pKa = 11.84 YY47 pKa = 6.43 EE48 pKa = 4.12 ARR50 pKa = 11.84 HH51 pKa = 4.56 GTGGKK56 pKa = 9.14 RR57 pKa = 11.84 AVFGVEE63 pKa = 4.34 GNSGTARR70 pKa = 11.84 ANHH73 pKa = 5.84 AADD76 pKa = 3.5 RR77 pKa = 11.84 RR78 pKa = 11.84 AYY80 pKa = 10.44 LL81 pKa = 3.71
Molecular weight: 9.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.765
IPC_protein 10.906
Toseland 10.877
ProMoST 10.965
Dawson 10.95
Bjellqvist 10.804
Wikipedia 11.286
Rodwell 10.891
Grimsley 11.008
Solomon 11.228
Lehninger 11.155
Nozaki 10.862
DTASelect 10.804
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.906
Patrickios 10.657
IPC_peptide 11.228
IPC2_peptide 10.101
IPC2.peptide.svr19 8.673
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
13764
37
1634
259.7
28.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.6 ± 0.652
0.857 ± 0.146
6.008 ± 0.175
5.863 ± 0.245
3.197 ± 0.155
8.515 ± 0.372
1.874 ± 0.155
4.483 ± 0.171
5.028 ± 0.335
7.905 ± 0.21
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.732 ± 0.192
4.199 ± 0.176
4.105 ± 0.242
4.432 ± 0.238
5.936 ± 0.283
5.979 ± 0.258
6.386 ± 0.313
6.917 ± 0.312
1.627 ± 0.119
3.357 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here