Aspergillus spelaeus tetramycovirus 1

Taxonomy: Viruses; Riboviria; Polymycoviridae; Polymycovirus; Aspergillus spelaeus polymycovirus 1

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3C4N7|A0A3G3C4N7_9VIRU Uncharacterized protein OS=Aspergillus spelaeus tetramycovirus 1 OX=2485922 PE=4 SV=1
MM1 pKa = 7.84ADD3 pKa = 3.99LARR6 pKa = 11.84LRR8 pKa = 11.84AIALSGDD15 pKa = 3.42LTVNLTAAILHH26 pKa = 6.34HH27 pKa = 6.71LSPDD31 pKa = 3.14QSEE34 pKa = 4.27TTRR37 pKa = 11.84YY38 pKa = 9.96GIADD42 pKa = 3.61VRR44 pKa = 11.84EE45 pKa = 4.02VMTWLSRR52 pKa = 11.84APGQFYY58 pKa = 11.02GDD60 pKa = 3.4VVAATGVTPAILSAIVDD77 pKa = 3.83EE78 pKa = 5.02MDD80 pKa = 4.89DD81 pKa = 3.9AACADD86 pKa = 3.99AATMANAMATPDD98 pKa = 3.89AEE100 pKa = 4.09WDD102 pKa = 3.55RR103 pKa = 11.84EE104 pKa = 3.95HH105 pKa = 7.58RR106 pKa = 11.84NRR108 pKa = 11.84FTTALATHH116 pKa = 6.39SADD119 pKa = 3.61AARR122 pKa = 11.84LAAQSSAEE130 pKa = 3.96FAALDD135 pKa = 3.73SLVEE139 pKa = 3.93AGYY142 pKa = 11.22ANEE145 pKa = 4.0DD146 pKa = 3.52HH147 pKa = 7.09ARR149 pKa = 11.84TAVNEE154 pKa = 3.91MNRR157 pKa = 11.84AYY159 pKa = 10.22GVNYY163 pKa = 10.02RR164 pKa = 11.84VLTRR168 pKa = 11.84QRR170 pKa = 11.84ARR172 pKa = 11.84SCVAIAVKK180 pKa = 10.5YY181 pKa = 9.9RR182 pKa = 11.84LPDD185 pKa = 3.22LTTAHH190 pKa = 7.48ILTPWCLSRR199 pKa = 11.84TAAMAYY205 pKa = 7.81VAVVARR211 pKa = 11.84HH212 pKa = 5.36IRR214 pKa = 11.84RR215 pKa = 11.84QQSEE219 pKa = 3.7ARR221 pKa = 11.84AGRR224 pKa = 11.84IARR227 pKa = 11.84TALSYY232 pKa = 11.09ASRR235 pKa = 11.84SGLDD239 pKa = 3.08AVRR242 pKa = 11.84SVVEE246 pKa = 3.76PAVAILTSFRR256 pKa = 11.84YY257 pKa = 9.66DD258 pKa = 3.2AEE260 pKa = 3.81RR261 pKa = 11.84LAFVDD266 pKa = 3.24TFDD269 pKa = 5.15RR270 pKa = 11.84EE271 pKa = 4.3RR272 pKa = 11.84EE273 pKa = 4.34SITTRR278 pKa = 11.84ASSVLVAFACIHH290 pKa = 5.64MCGEE294 pKa = 4.02TDD296 pKa = 3.59VLIRR300 pKa = 11.84AAQLRR305 pKa = 11.84ADD307 pKa = 4.06VVPDD311 pKa = 3.17QTPPHH316 pKa = 6.7PSVVEE321 pKa = 3.9YY322 pKa = 10.86TEE324 pKa = 4.33TVDD327 pKa = 5.69DD328 pKa = 4.41EE329 pKa = 4.59QTGLLMFSRR338 pKa = 11.84LLHH341 pKa = 6.1AARR344 pKa = 11.84AQRR347 pKa = 11.84DD348 pKa = 3.44QSRR351 pKa = 11.84AGPRR355 pKa = 11.84VNKK358 pKa = 10.24DD359 pKa = 2.92LMLGYY364 pKa = 9.82VARR367 pKa = 11.84GKK369 pKa = 10.45KK370 pKa = 9.66KK371 pKa = 10.29SEE373 pKa = 4.28RR374 pKa = 11.84NTMACINRR382 pKa = 11.84VEE384 pKa = 4.25EE385 pKa = 4.21AVSLLRR391 pKa = 11.84SKK393 pKa = 10.78KK394 pKa = 10.36ADD396 pKa = 3.35VLNTLFVIEE405 pKa = 4.4WGGEE409 pKa = 3.63FDD411 pKa = 4.36YY412 pKa = 9.22PTVMAALAVARR423 pKa = 11.84LDD425 pKa = 3.48VCVDD429 pKa = 3.7IGPSGIDD436 pKa = 3.33LPGADD441 pKa = 4.06VVADD445 pKa = 4.15DD446 pKa = 4.23TPEE449 pKa = 3.97EE450 pKa = 4.22YY451 pKa = 10.38NYY453 pKa = 11.42SLLISSAEE461 pKa = 3.82EE462 pKa = 3.52RR463 pKa = 11.84MMPRR467 pKa = 11.84MPRR470 pKa = 11.84ISYY473 pKa = 9.75PKK475 pKa = 10.23DD476 pKa = 3.21EE477 pKa = 4.63PLEE480 pKa = 4.46SKK482 pKa = 10.53LLRR485 pKa = 11.84VAEE488 pKa = 4.84FYY490 pKa = 11.19CRR492 pKa = 11.84DD493 pKa = 3.21GRR495 pKa = 11.84SNIAYY500 pKa = 9.5ISGGTLQHH508 pKa = 6.63EE509 pKa = 4.75GTPVSVCVDD518 pKa = 3.23TNNRR522 pKa = 11.84LDD524 pKa = 4.52AIRR527 pKa = 11.84NATAQVPINFACAEE541 pKa = 4.01ALIPPLCPHH550 pKa = 7.3GAQAPLEE557 pKa = 4.46EE558 pKa = 4.32YY559 pKa = 10.86LDD561 pKa = 3.78TSSLIDD567 pKa = 4.05EE568 pKa = 4.84EE569 pKa = 5.0CPNCNAHH576 pKa = 5.71YY577 pKa = 10.29RR578 pKa = 11.84SVALLSAACAKK589 pKa = 10.33GHH591 pKa = 5.0TRR593 pKa = 11.84VVKK596 pKa = 10.03PRR598 pKa = 11.84SSYY601 pKa = 9.95AHH603 pKa = 6.33NFHH606 pKa = 6.79VSLEE610 pKa = 4.25LVPGHH615 pKa = 5.88PTCPEE620 pKa = 3.93DD621 pKa = 3.49TLTTIDD627 pKa = 4.03SMVAANVCRR636 pKa = 11.84NADD639 pKa = 3.3WGDD642 pKa = 3.65TPNFPLGGGNPSSEE656 pKa = 4.37NYY658 pKa = 9.63RR659 pKa = 11.84KK660 pKa = 10.41LNAITMKK667 pKa = 10.34HH668 pKa = 5.55VYY670 pKa = 10.27ACLSSVATGPFDD682 pKa = 4.41YY683 pKa = 11.22DD684 pKa = 3.78DD685 pKa = 4.1MASIAASIAPP695 pKa = 3.85

Molecular weight: 75.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3C4N5|A0A3G3C4N5_9VIRU Methyltransferase OS=Aspergillus spelaeus tetramycovirus 1 OX=2485922 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 6.43LLHH5 pKa = 7.2SDD7 pKa = 4.33PEE9 pKa = 4.36QACSRR14 pKa = 11.84IHH16 pKa = 6.42GLVSDD21 pKa = 4.7RR22 pKa = 11.84LRR24 pKa = 11.84KK25 pKa = 9.65RR26 pKa = 11.84GSSRR30 pKa = 11.84PVKK33 pKa = 10.43VDD35 pKa = 3.59LEE37 pKa = 4.39GSPSAKK43 pKa = 10.03SAGNSAPSSGDD54 pKa = 2.8GGLALLRR61 pKa = 11.84RR62 pKa = 11.84EE63 pKa = 4.41VKK65 pKa = 10.42RR66 pKa = 11.84NSGVYY71 pKa = 10.17GSYY74 pKa = 10.33QFAAGPTKK82 pKa = 10.61RR83 pKa = 11.84PGGQAFSVDD92 pKa = 3.5LGGGLWALGQSKK104 pKa = 10.24AVAVAAARR112 pKa = 11.84ICRR115 pKa = 11.84VLGRR119 pKa = 11.84HH120 pKa = 5.89EE121 pKa = 4.86DD122 pKa = 3.37LVRR125 pKa = 11.84PYY127 pKa = 10.21IYY129 pKa = 10.29YY130 pKa = 8.69WASGSSPRR138 pKa = 11.84SAADD142 pKa = 2.85ISEE145 pKa = 4.35KK146 pKa = 10.67VWDD149 pKa = 4.18GRR151 pKa = 11.84LGPHH155 pKa = 6.61EE156 pKa = 5.03DD157 pKa = 4.25APPEE161 pKa = 4.15PATKK165 pKa = 10.49AKK167 pKa = 10.11TEE169 pKa = 3.93ATKK172 pKa = 10.91

Molecular weight: 18.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2075

172

760

518.8

56.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.422 ± 1.521

1.253 ± 0.491

7.181 ± 0.447

4.916 ± 0.356

2.843 ± 0.442

7.229 ± 1.304

2.602 ± 0.239

3.181 ± 0.507

3.229 ± 0.665

9.542 ± 0.688

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.582

2.554 ± 0.592

5.928 ± 0.538

1.928 ± 0.101

7.855 ± 0.727

7.518 ± 0.924

5.446 ± 0.653

8.53 ± 1.172

0.867 ± 0.157

3.566 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski