Streptococcus phage phi29961

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T9K1|A0A6M3T9K1_9CAUD Uncharacterized protein OS=Streptococcus phage phi29961 OX=2729190 GN=phi29961_0024 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.52LKK4 pKa = 10.66DD5 pKa = 3.35ILEE8 pKa = 4.55LGTYY12 pKa = 9.43GFNPNCKK19 pKa = 9.81VEE21 pKa = 4.25IFCMDD26 pKa = 3.23NFEE29 pKa = 4.53EE30 pKa = 4.31RR31 pKa = 11.84LEE33 pKa = 4.14NEE35 pKa = 4.24GFDD38 pKa = 5.31EE39 pKa = 5.48ILIPQNDD46 pKa = 3.8DD47 pKa = 3.08AKK49 pKa = 10.01IYY51 pKa = 9.96PYY53 pKa = 11.23AFLIEE58 pKa = 4.24DD59 pKa = 4.28SILIAMTEE67 pKa = 3.68VDD69 pKa = 5.49DD70 pKa = 6.92DD71 pKa = 4.33INEE74 pKa = 4.24CC75 pKa = 4.39

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T9L1|A0A6M3T9L1_9CAUD Head closure protein OS=Streptococcus phage phi29961 OX=2729190 GN=phi29961_0044 PE=4 SV=1
MM1 pKa = 7.73NDD3 pKa = 3.44FKK5 pKa = 11.42AKK7 pKa = 9.87QVWKK11 pKa = 9.47MRR13 pKa = 11.84NEE15 pKa = 3.7GLGYY19 pKa = 10.37RR20 pKa = 11.84KK21 pKa = 9.31IANLLDD27 pKa = 4.0LSLTAVKK34 pKa = 9.88RR35 pKa = 11.84YY36 pKa = 10.1CSTNPHH42 pKa = 6.13LKK44 pKa = 10.39GYY46 pKa = 8.41GQAVVAMISDD56 pKa = 4.37GQRR59 pKa = 11.84NGQLCKK65 pKa = 10.59EE66 pKa = 4.13CLKK69 pKa = 10.69PIEE72 pKa = 4.2QPRR75 pKa = 11.84KK76 pKa = 9.23GRR78 pKa = 11.84TKK80 pKa = 10.14QFCSDD85 pKa = 2.85SCRR88 pKa = 11.84RR89 pKa = 11.84KK90 pKa = 10.14NWVTQHH96 pKa = 6.36PEE98 pKa = 3.67KK99 pKa = 10.71ADD101 pKa = 3.31SHH103 pKa = 6.84VCEE106 pKa = 4.5LCGKK110 pKa = 7.83TFTAYY115 pKa = 10.23GSPRR119 pKa = 11.84RR120 pKa = 11.84KK121 pKa = 9.41FCSRR125 pKa = 11.84TCARR129 pKa = 11.84TPRR132 pKa = 11.84RR133 pKa = 11.84TT134 pKa = 3.24

Molecular weight: 15.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

17443

45

1039

300.7

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.123 ± 0.361

0.952 ± 0.141

5.63 ± 0.258

7.751 ± 0.444

4.197 ± 0.233

6.335 ± 0.346

1.76 ± 0.143

6.69 ± 0.288

8.37 ± 0.655

8.869 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.146

5.137 ± 0.24

3.102 ± 0.147

4.242 ± 0.203

4.334 ± 0.272

6.799 ± 0.281

6.06 ± 0.259

6.381 ± 0.321

1.227 ± 0.099

3.698 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski