Yersinia phage Yepe2
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3VCK1|B3VCK1_9CAUD Gp6 OS=Yersinia phage Yepe2 OX=532078 GN=6 PE=4 SV=1
MM1 pKa = 7.66 SLLTDD6 pKa = 3.63 NVQVTRR12 pKa = 11.84 EE13 pKa = 4.1 AWNDD17 pKa = 3.06 MQMYY21 pKa = 9.15 IQSLEE26 pKa = 3.91 KK27 pKa = 10.76 DD28 pKa = 3.65 QEE30 pKa = 4.08 FLDD33 pKa = 3.74 ALRR36 pKa = 11.84 ACGVDD41 pKa = 3.04 NWDD44 pKa = 3.87 GYY46 pKa = 11.34 LDD48 pKa = 3.82 AVEE51 pKa = 4.45 MVCRR55 pKa = 11.84 EE56 pKa = 4.11 DD57 pKa = 5.05 DD58 pKa = 3.57 LL59 pKa = 6.7
Molecular weight: 6.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.069
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 3.058
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|B3VCI5|B3VCI5_9CAUD Gp1.7 OS=Yersinia phage Yepe2 OX=532078 GN=1.7 PE=4 SV=1
MM1 pKa = 7.61 LRR3 pKa = 11.84 LHH5 pKa = 6.6 FNKK8 pKa = 10.45 SNGIYY13 pKa = 9.76 SVRR16 pKa = 11.84 DD17 pKa = 3.33 SGRR20 pKa = 11.84 SLVKK24 pKa = 9.83 ATEE27 pKa = 3.87 KK28 pKa = 10.51 HH29 pKa = 5.28 AVIPKK34 pKa = 9.9 VLGATIEE41 pKa = 3.98 LAPRR45 pKa = 11.84 VHH47 pKa = 7.02 AVITTGLFKK56 pKa = 10.67 EE57 pKa = 4.35 ATTGSRR63 pKa = 11.84 PFIPVLVTRR72 pKa = 11.84 FPKK75 pKa = 9.72 VRR77 pKa = 11.84 LVIEE81 pKa = 4.63 CIKK84 pKa = 10.8 EE85 pKa = 4.08 VFF87 pKa = 3.46
Molecular weight: 9.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 9.575
IPC_protein 9.838
Toseland 10.76
ProMoST 10.277
Dawson 10.818
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.257
Grimsley 10.847
Solomon 10.906
Lehninger 10.891
Nozaki 10.73
DTASelect 10.423
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.745
Patrickios 11.023
IPC_peptide 10.921
IPC2_peptide 9.092
IPC2.peptide.svr19 8.674
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
12071
25
1315
262.4
29.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.889 ± 0.53
1.019 ± 0.177
6.271 ± 0.255
7.166 ± 0.318
3.513 ± 0.171
7.804 ± 0.265
1.806 ± 0.187
4.755 ± 0.188
6.917 ± 0.298
8.235 ± 0.311
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.281 ± 0.198
4.772 ± 0.211
3.62 ± 0.229
3.786 ± 0.236
5.335 ± 0.199
6.023 ± 0.342
5.393 ± 0.18
6.702 ± 0.358
1.475 ± 0.193
3.239 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here