Foxtail mosaic virus
Average proteome isoelectric point is 7.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P22170|TGB2_FXMV Movement protein TGB2 OS=Foxtail mosaic virus OX=12179 GN=ORF3 PE=3 SV=2
MM1 pKa = 7.94 DD2 pKa = 3.99 SEE4 pKa = 4.23 IVEE7 pKa = 4.47 RR8 pKa = 11.84 LTKK11 pKa = 10.6 LGFVKK16 pKa = 10.17 TSHH19 pKa = 4.75 THH21 pKa = 5.21 IAGEE25 pKa = 4.05 PLVIHH30 pKa = 6.67 AVAGAGKK37 pKa = 6.67 TTLLRR42 pKa = 11.84 SLLEE46 pKa = 4.02 LPGVEE51 pKa = 4.05 VFTGGEE57 pKa = 4.01 HH58 pKa = 7.71 DD59 pKa = 4.47 PPNLSGKK66 pKa = 8.9 YY67 pKa = 8.66 IRR69 pKa = 11.84 CAAPPVAGAYY79 pKa = 9.96 NILDD83 pKa = 4.33 EE84 pKa = 4.54 YY85 pKa = 10.05 PAYY88 pKa = 9.71 PNWRR92 pKa = 11.84 SQPWNVLIADD102 pKa = 3.73 NLQYY106 pKa = 10.88 KK107 pKa = 9.96 EE108 pKa = 3.95 PTARR112 pKa = 11.84 AHH114 pKa = 4.45 YY115 pKa = 7.92 TCNRR119 pKa = 11.84 THH121 pKa = 7.7 RR122 pKa = 11.84 LGQLTVDD129 pKa = 3.71 ALRR132 pKa = 11.84 RR133 pKa = 11.84 VGFDD137 pKa = 2.64 ITFAGTQTEE146 pKa = 4.62 DD147 pKa = 3.23 YY148 pKa = 10.45 GFQEE152 pKa = 3.9 GHH154 pKa = 7.4 LYY156 pKa = 10.07 TSQFYY161 pKa = 10.45 GQVISLDD168 pKa = 3.86 TQAHH172 pKa = 6.59 KK173 pKa = 10.48 IAVRR177 pKa = 11.84 HH178 pKa = 5.26 GLAPLSALEE187 pKa = 4.08 TRR189 pKa = 11.84 GLEE192 pKa = 4.01 FDD194 pKa = 3.68 EE195 pKa = 4.65 TTVITTKK202 pKa = 10.58 TSLEE206 pKa = 4.17 EE207 pKa = 3.85 VKK209 pKa = 10.31 DD210 pKa = 3.43 RR211 pKa = 11.84 HH212 pKa = 4.97 MVYY215 pKa = 10.4 VALTRR220 pKa = 11.84 HH221 pKa = 5.99 RR222 pKa = 11.84 RR223 pKa = 11.84 TCHH226 pKa = 6.85 LYY228 pKa = 8.32 TAHH231 pKa = 7.14 FAPSAA236 pKa = 3.52
Molecular weight: 26.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.318
IPC2_protein 6.211
IPC_protein 6.376
Toseland 6.795
ProMoST 6.781
Dawson 6.722
Bjellqvist 6.664
Wikipedia 6.751
Rodwell 6.722
Grimsley 6.971
Solomon 6.737
Lehninger 6.737
Nozaki 6.985
DTASelect 7.176
Thurlkill 7.205
EMBOSS 7.19
Sillero 7.117
Patrickios 3.999
IPC_peptide 6.751
IPC2_peptide 7.029
IPC2.peptide.svr19 6.909
Protein with the highest isoelectric point:
>sp|P22171|TGB3_FXMV Movement protein TGBp3 OS=Foxtail mosaic virus OX=12179 GN=ORF4 PE=3 SV=2
MM1 pKa = 7.26 SLSHH5 pKa = 6.11 GTGAPAISTPLTLRR19 pKa = 11.84 PPPDD23 pKa = 3.26 NTKK26 pKa = 10.8 AILTIAIGIAASLVFFMLTRR46 pKa = 11.84 NNLPHH51 pKa = 7.55 VGDD54 pKa = 4.91 NIHH57 pKa = 6.41 SLPHH61 pKa = 5.78 GGSYY65 pKa = 10.05 IDD67 pKa = 3.58 GTKK70 pKa = 10.3 SINYY74 pKa = 8.21 RR75 pKa = 11.84 PPASRR80 pKa = 11.84 YY81 pKa = 8.64 PSSNLLAFAPPILAAVLFFLTQPYY105 pKa = 10.08 LATRR109 pKa = 11.84 RR110 pKa = 11.84 SRR112 pKa = 11.84 CVRR115 pKa = 11.84 CFVVHH120 pKa = 6.42 GACTNHH126 pKa = 5.36 TT127 pKa = 3.9
Molecular weight: 13.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.118
IPC2_protein 9.619
IPC_protein 10.321
Toseland 9.867
ProMoST 9.838
Dawson 10.204
Bjellqvist 10.043
Wikipedia 10.467
Rodwell 10.277
Grimsley 10.321
Solomon 10.277
Lehninger 10.233
Nozaki 10.058
DTASelect 10.014
Thurlkill 10.028
EMBOSS 10.321
Sillero 10.16
Patrickios 7.483
IPC_peptide 10.262
IPC2_peptide 9.428
IPC2.peptide.svr19 8.281
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1965
52
1335
393.0
44.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.601 ± 1.281
1.425 ± 0.337
5.038 ± 0.632
6.056 ± 1.187
4.173 ± 0.389
4.682 ± 0.657
3.155 ± 0.923
4.885 ± 0.365
5.7 ± 1.082
9.618 ± 0.715
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.985 ± 0.415
4.224 ± 0.639
7.125 ± 0.615
4.071 ± 0.467
5.598 ± 0.214
5.191 ± 0.545
7.837 ± 0.623
5.598 ± 0.442
1.221 ± 0.257
3.817 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here