Candidatus Mycoplasma haemobos

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9QDZ4|A0A1A9QDZ4_9MOLU Uncharacterized protein OS=Candidatus Mycoplasma haemobos OX=432608 GN=A6V39_00240 PE=4 SV=1
MM1 pKa = 7.4LPVATVTTATILTTSLISSGKK22 pKa = 9.89VDD24 pKa = 4.52LDD26 pKa = 3.44KK27 pKa = 11.4KK28 pKa = 10.95KK29 pKa = 10.83EE30 pKa = 4.04NEE32 pKa = 4.01DD33 pKa = 3.31QEE35 pKa = 5.8LNDD38 pKa = 3.84FVKK41 pKa = 10.63PPEE44 pKa = 4.34DD45 pKa = 4.09FEE47 pKa = 4.77DD48 pKa = 3.56EE49 pKa = 4.36KK50 pKa = 11.08IFYY53 pKa = 10.5SKK55 pKa = 11.03EE56 pKa = 3.75GDD58 pKa = 3.48SDD60 pKa = 3.84NLRR63 pKa = 11.84TLDD66 pKa = 3.83KK67 pKa = 10.54DD68 pKa = 3.6TAKK71 pKa = 10.7KK72 pKa = 10.15IEE74 pKa = 4.17EE75 pKa = 4.05LMQKK79 pKa = 10.38NGGKK83 pKa = 9.76LEE85 pKa = 4.28KK86 pKa = 10.8GEE88 pKa = 4.03QLGDD92 pKa = 3.46TVQAPNPEE100 pKa = 4.11INPNVPEE107 pKa = 4.1NQPQNVGKK115 pKa = 10.26ADD117 pKa = 3.68SPEE120 pKa = 3.97ITSKK124 pKa = 11.48AEE126 pKa = 4.24DD127 pKa = 3.61KK128 pKa = 11.49DD129 pKa = 3.67MTSVPKK135 pKa = 10.82GNNKK139 pKa = 10.3DD140 pKa = 3.5EE141 pKa = 4.83GDD143 pKa = 4.1TISEE147 pKa = 4.4TKK149 pKa = 9.54DD150 pKa = 3.17TLISTSEE157 pKa = 4.02STDD160 pKa = 3.51DD161 pKa = 4.67DD162 pKa = 4.08FTNGRR167 pKa = 11.84DD168 pKa = 4.0DD169 pKa = 4.01PQQPSLDD176 pKa = 4.04DD177 pKa = 4.19DD178 pKa = 4.83KK179 pKa = 11.76KK180 pKa = 11.17DD181 pKa = 3.38SDD183 pKa = 4.28VNEE186 pKa = 4.29TPPLDD191 pKa = 3.77TEE193 pKa = 4.6EE194 pKa = 4.59NDD196 pKa = 3.54LNKK199 pKa = 10.59GKK201 pKa = 10.11EE202 pKa = 4.28GPEE205 pKa = 3.59IRR207 pKa = 11.84DD208 pKa = 3.48DD209 pKa = 3.57KK210 pKa = 11.16SQNEE214 pKa = 4.74GIGDD218 pKa = 3.88VNSVVQKK225 pKa = 9.44TLEE228 pKa = 4.37PQSSTEE234 pKa = 3.98EE235 pKa = 4.14NGVEE239 pKa = 4.32TNDD242 pKa = 3.74DD243 pKa = 3.72TSVDD247 pKa = 3.55PVTNPEE253 pKa = 3.95MKK255 pKa = 10.63NEE257 pKa = 4.04VGEE260 pKa = 4.23ATHH263 pKa = 6.47QDD265 pKa = 2.89VSMEE269 pKa = 4.09AKK271 pKa = 10.37GDD273 pKa = 3.76LGKK276 pKa = 11.13GPNFDD281 pKa = 3.6TSKK284 pKa = 10.96EE285 pKa = 4.24SGDD288 pKa = 3.89SKK290 pKa = 11.55GLDD293 pKa = 3.63GGNDD297 pKa = 3.69DD298 pKa = 5.51QISDD302 pKa = 4.23GNSDD306 pKa = 3.78RR307 pKa = 11.84YY308 pKa = 10.56SEE310 pKa = 4.14EE311 pKa = 3.8AIKK314 pKa = 10.57RR315 pKa = 11.84LEE317 pKa = 3.95QMQGEE322 pKa = 4.15LSEE325 pKa = 4.46FEE327 pKa = 5.04LRR329 pKa = 11.84LKK331 pKa = 10.63QLIDD335 pKa = 3.22

Molecular weight: 36.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A9QES2|A0A1A9QES2_9MOLU Uncharacterized protein OS=Candidatus Mycoplasma haemobos OX=432608 GN=A6V39_01990 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.3IKK4 pKa = 10.44IKK6 pKa = 9.21TKK8 pKa = 9.45KK9 pKa = 10.26AFSKK13 pKa = 10.58RR14 pKa = 11.84IRR16 pKa = 11.84VLGNGKK22 pKa = 9.75LKK24 pKa = 10.38RR25 pKa = 11.84KK26 pKa = 9.23HH27 pKa = 4.81SHH29 pKa = 6.44RR30 pKa = 11.84SHH32 pKa = 6.72LASNKK37 pKa = 7.0STKK40 pKa = 8.29QKK42 pKa = 9.73RR43 pKa = 11.84QARR46 pKa = 11.84SSVMLNKK53 pKa = 10.26SQIKK57 pKa = 9.73RR58 pKa = 11.84AYY60 pKa = 9.94QLLQGG65 pKa = 4.28

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1109

0

1109

274467

55

2048

247.5

28.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.599 ± 0.067

1.317 ± 0.028

5.648 ± 0.064

7.725 ± 0.082

4.571 ± 0.079

5.581 ± 0.068

1.354 ± 0.027

7.313 ± 0.078

10.93 ± 0.1

9.529 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.393 ± 0.027

6.424 ± 0.063

2.825 ± 0.044

3.136 ± 0.043

3.174 ± 0.047

7.428 ± 0.07

5.801 ± 0.075

5.011 ± 0.054

1.624 ± 0.035

3.615 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski