Phocoena phocoena papillomavirus 2
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2VIR3|F2VIR3_9PAPI Replication protein E1 OS=Phocoena phocoena papillomavirus 2 OX=706526 GN=E1 PE=3 SV=1
MM1 pKa = 7.78 ADD3 pKa = 3.67 DD4 pKa = 4.43 PRR6 pKa = 11.84 TIKK9 pKa = 10.46 DD10 pKa = 3.16 LCKK13 pKa = 10.86 NFDD16 pKa = 3.97 LSVQALLILCVFCKK30 pKa = 10.45 KK31 pKa = 10.25 QLTDD35 pKa = 3.52 LEE37 pKa = 4.39 IWSFSHH43 pKa = 5.92 KK44 pKa = 7.93 QLRR47 pKa = 11.84 VVWKK51 pKa = 10.33 KK52 pKa = 10.13 GFPFAACKK60 pKa = 10.25 KK61 pKa = 8.81 CLEE64 pKa = 4.07 VCALVDD70 pKa = 3.24 CWRR73 pKa = 11.84 NFQRR77 pKa = 11.84 SATAKK82 pKa = 10.0 QVEE85 pKa = 4.6 EE86 pKa = 4.32 DD87 pKa = 3.27 TGKK90 pKa = 10.85 ALGDD94 pKa = 3.51 LDD96 pKa = 4.35 VRR98 pKa = 11.84 CMGCYY103 pKa = 10.7 KK104 pKa = 11.03 LMTTLEE110 pKa = 4.07 KK111 pKa = 10.35 AYY113 pKa = 10.31 QLEE116 pKa = 4.59 DD117 pKa = 3.12 EE118 pKa = 4.67 KK119 pKa = 11.16 QFCKK123 pKa = 10.23 IAGYY127 pKa = 9.07 WKK129 pKa = 10.21 GYY131 pKa = 9.78 CINCLTDD138 pKa = 3.71 PPPLVSWFNYY148 pKa = 8.78 AVTVGGPPPLITWGFDD164 pKa = 3.23 PPRR167 pKa = 11.84 RR168 pKa = 11.84 PAQSVSSGSSWTITTSTSSSGRR190 pKa = 11.84 EE191 pKa = 3.98 PEE193 pKa = 4.51 PDD195 pKa = 3.68 DD196 pKa = 5.56 PEE198 pKa = 7.13 DD199 pKa = 4.68 GDD201 pKa = 4.56 SSDD204 pKa = 4.42 DD205 pKa = 3.73 QEE207 pKa = 4.41 EE208 pKa = 4.06 MLII211 pKa = 4.27
Molecular weight: 23.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.801
IPC2_protein 4.927
IPC_protein 4.825
Toseland 4.66
ProMoST 4.94
Dawson 4.787
Bjellqvist 4.927
Wikipedia 4.673
Rodwell 4.66
Grimsley 4.571
Solomon 4.787
Lehninger 4.736
Nozaki 4.889
DTASelect 5.092
Thurlkill 4.673
EMBOSS 4.698
Sillero 4.94
Patrickios 3.745
IPC_peptide 4.787
IPC2_peptide 4.927
IPC2.peptide.svr19 4.949
Protein with the highest isoelectric point:
>tr|F2VIR7|F2VIR7_9PAPI Major capsid protein L1 OS=Phocoena phocoena papillomavirus 2 OX=706526 GN=L1 PE=3 SV=1
MM1 pKa = 7.62 PLILVVAFTYY11 pKa = 10.26 IHH13 pKa = 6.79 IFAGRR18 pKa = 11.84 NANACIFNIFQMLHH32 pKa = 6.55 LPPPAPLSRR41 pKa = 11.84 VLHH44 pKa = 5.73 TDD46 pKa = 3.04 EE47 pKa = 4.63 FVSRR51 pKa = 11.84 TSTFYY56 pKa = 10.02 HH57 pKa = 6.15 CQSEE61 pKa = 4.12 RR62 pKa = 11.84 LITIGHH68 pKa = 7.38 PFYY71 pKa = 10.82 KK72 pKa = 10.53 VQDD75 pKa = 3.9 GGNVVAEE82 pKa = 4.13 KK83 pKa = 10.73 VSPNQYY89 pKa = 9.42 RR90 pKa = 11.84 VFRR93 pKa = 11.84 VTLPDD98 pKa = 4.27 PNVLALTDD106 pKa = 3.48 SSLYY110 pKa = 10.46 NPEE113 pKa = 3.88 KK114 pKa = 10.63 EE115 pKa = 3.85 RR116 pKa = 11.84 LVWILRR122 pKa = 11.84 GIDD125 pKa = 3.39 VGRR128 pKa = 11.84 GGPLGIGVTGHH139 pKa = 7.4 PYY141 pKa = 10.66 LDD143 pKa = 3.52 KK144 pKa = 11.41 LKK146 pKa = 10.88 DD147 pKa = 3.51 AEE149 pKa = 4.51 NPNGSYY155 pKa = 11.2 NKK157 pKa = 10.32 GSDD160 pKa = 3.42 DD161 pKa = 3.7 ARR163 pKa = 11.84 QNVCMDD169 pKa = 4.32 PKK171 pKa = 10.31 SVQMLILGCAPAIGQHH187 pKa = 4.91 WDD189 pKa = 3.15 KK190 pKa = 11.92 AEE192 pKa = 4.06 ACASDD197 pKa = 4.05 RR198 pKa = 11.84 DD199 pKa = 4.17 AVDD202 pKa = 3.32 ACPPLEE208 pKa = 4.59 LKK210 pKa = 9.19 NTTIEE215 pKa = 4.51 DD216 pKa = 3.36 GDD218 pKa = 3.81 MFDD221 pKa = 5.32 IGFGCMNNSTLQATFSAVPLDD242 pKa = 3.49 ITNTTTKK249 pKa = 10.54 HH250 pKa = 5.62 PDD252 pKa = 3.03 ILKK255 pKa = 7.88 MTGDD259 pKa = 3.85 IYY261 pKa = 11.54 GNTCWFCVSRR271 pKa = 11.84 EE272 pKa = 3.96 QMFARR277 pKa = 11.84 HH278 pKa = 5.04 LWSRR282 pKa = 11.84 NGDD285 pKa = 3.55 NGDD288 pKa = 4.16 PVPHH292 pKa = 6.87 TDD294 pKa = 2.97 GHH296 pKa = 6.76 KK297 pKa = 10.09 DD298 pKa = 3.0 TSLYY302 pKa = 10.94 LSGTGDD308 pKa = 3.97 TKK310 pKa = 11.15 TMATPVYY317 pKa = 9.7 FNTPSGSLLTSEE329 pKa = 4.25 TQLFNRR335 pKa = 11.84 PFWMQRR341 pKa = 11.84 AQGLNNGVCWNNTLFVTVADD361 pKa = 3.92 NTRR364 pKa = 11.84 GTNLNISVAKK374 pKa = 10.43 QEE376 pKa = 4.21 GKK378 pKa = 9.97 NAEE381 pKa = 4.41 SYY383 pKa = 8.1 TASNYY388 pKa = 8.41 KK389 pKa = 10.07 HH390 pKa = 6.24 YY391 pKa = 10.73 NRR393 pKa = 11.84 HH394 pKa = 5.18 CEE396 pKa = 3.68 IYY398 pKa = 9.47 EE399 pKa = 4.11 LEE401 pKa = 5.3 FILQLATVALTPEE414 pKa = 4.56 ALSHH418 pKa = 5.61 LHH420 pKa = 7.03 AMDD423 pKa = 3.72 STILTKK429 pKa = 10.34 WNLGFTASNATAVEE443 pKa = 3.98 STYY446 pKa = 10.75 RR447 pKa = 11.84 YY448 pKa = 9.73 INSKK452 pKa = 8.25 ATKK455 pKa = 9.69 CPIDD459 pKa = 3.64 IPEE462 pKa = 4.84 PEE464 pKa = 4.99 PKK466 pKa = 10.1 DD467 pKa = 3.44 KK468 pKa = 11.46 YY469 pKa = 11.25 ADD471 pKa = 3.49 LTFWNVDD478 pKa = 3.21 VSKK481 pKa = 11.0 SLSKK485 pKa = 10.78 DD486 pKa = 3.23 LSAFPLGRR494 pKa = 11.84 KK495 pKa = 8.68 FLYY498 pKa = 10.02 QAGLQNGATRR508 pKa = 11.84 SATKK512 pKa = 10.4 RR513 pKa = 11.84 SATKK517 pKa = 10.06 TSTKK521 pKa = 10.31 VPTKK525 pKa = 10.1 RR526 pKa = 11.84 KK527 pKa = 9.53 RR528 pKa = 11.84 RR529 pKa = 3.71
Molecular weight: 58.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.807
IPC2_protein 7.029
IPC_protein 6.971
Toseland 6.825
ProMoST 7.614
Dawson 7.614
Bjellqvist 7.907
Wikipedia 7.541
Rodwell 7.629
Grimsley 6.912
Solomon 7.658
Lehninger 7.673
Nozaki 8.17
DTASelect 7.805
Thurlkill 7.849
EMBOSS 7.878
Sillero 8.126
Patrickios 4.151
IPC_peptide 7.658
IPC2_peptide 7.293
IPC2.peptide.svr19 7.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2515
171
638
419.2
46.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.203 ± 0.354
2.783 ± 0.845
6.481 ± 0.559
5.129 ± 0.42
3.658 ± 0.374
7.197 ± 0.373
2.584 ± 0.511
4.493 ± 0.557
5.288 ± 0.889
8.986 ± 0.611
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.303
3.817 ± 0.638
6.64 ± 0.71
4.334 ± 0.88
5.01 ± 0.391
7.674 ± 0.704
7.634 ± 0.908
5.686 ± 0.053
1.551 ± 0.375
2.863 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here