Amino acid dipepetide frequency for Salmonella phage vB_SPuM_SP116

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.11AlaAla: 5.11 ± 0.592
0.515AlaCys: 0.515 ± 0.148
3.961AlaAsp: 3.961 ± 0.372
4.001AlaGlu: 4.001 ± 0.366
2.614AlaPhe: 2.614 ± 0.337
5.031AlaGly: 5.031 ± 0.499
1.505AlaHis: 1.505 ± 0.339
4.951AlaIle: 4.951 ± 0.452
5.387AlaLys: 5.387 ± 0.491
5.823AlaLeu: 5.823 ± 0.581
2.496AlaMet: 2.496 ± 0.356
3.565AlaAsn: 3.565 ± 0.357
1.664AlaPro: 1.664 ± 0.301
2.733AlaGln: 2.733 ± 0.412
2.575AlaArg: 2.575 ± 0.373
4.159AlaSer: 4.159 ± 0.436
4.159AlaThr: 4.159 ± 0.48
5.15AlaVal: 5.15 ± 0.491
0.951AlaTrp: 0.951 ± 0.192
3.05AlaTyr: 3.05 ± 0.372
0.0AlaXaa: 0.0 ± 0.0
Cys
0.475CysAla: 0.475 ± 0.175
0.198CysCys: 0.198 ± 0.096
0.594CysAsp: 0.594 ± 0.144
1.109CysGlu: 1.109 ± 0.229
0.673CysPhe: 0.673 ± 0.171
1.07CysGly: 1.07 ± 0.214
0.396CysHis: 0.396 ± 0.122
0.436CysIle: 0.436 ± 0.13
1.426CysLys: 1.426 ± 0.264
0.951CysLeu: 0.951 ± 0.186
0.357CysMet: 0.357 ± 0.115
0.753CysAsn: 0.753 ± 0.181
0.555CysPro: 0.555 ± 0.149
0.475CysGln: 0.475 ± 0.181
0.555CysArg: 0.555 ± 0.149
0.871CysSer: 0.871 ± 0.211
0.594CysThr: 0.594 ± 0.132
0.832CysVal: 0.832 ± 0.194
0.079CysTrp: 0.079 ± 0.057
0.673CysTyr: 0.673 ± 0.161
0.0CysXaa: 0.0 ± 0.0
Asp
4.357AspAla: 4.357 ± 0.395
0.555AspCys: 0.555 ± 0.151
3.724AspAsp: 3.724 ± 0.509
4.635AspGlu: 4.635 ± 0.465
3.446AspPhe: 3.446 ± 0.342
5.11AspGly: 5.11 ± 0.396
0.713AspHis: 0.713 ± 0.171
4.159AspIle: 4.159 ± 0.444
4.397AspLys: 4.397 ± 0.367
5.11AspLeu: 5.11 ± 0.425
1.783AspMet: 1.783 ± 0.259
3.803AspAsn: 3.803 ± 0.437
1.664AspPro: 1.664 ± 0.274
0.673AspGln: 0.673 ± 0.155
2.02AspArg: 2.02 ± 0.247
4.397AspSer: 4.397 ± 0.41
3.684AspThr: 3.684 ± 0.319
4.714AspVal: 4.714 ± 0.503
1.228AspTrp: 1.228 ± 0.205
2.733AspTyr: 2.733 ± 0.352
0.0AspXaa: 0.0 ± 0.0
Glu
5.585GluAla: 5.585 ± 0.545
0.713GluCys: 0.713 ± 0.157
4.516GluAsp: 4.516 ± 0.525
5.902GluGlu: 5.902 ± 0.605
2.931GluPhe: 2.931 ± 0.376
4.357GluGly: 4.357 ± 0.407
1.545GluHis: 1.545 ± 0.288
4.199GluIle: 4.199 ± 0.508
5.308GluLys: 5.308 ± 0.554
6.259GluLeu: 6.259 ± 0.446
2.812GluMet: 2.812 ± 0.314
3.763GluAsn: 3.763 ± 0.37
1.624GluPro: 1.624 ± 0.241
2.139GluGln: 2.139 ± 0.279
2.852GluArg: 2.852 ± 0.295
3.684GluSer: 3.684 ± 0.415
3.565GluThr: 3.565 ± 0.292
4.357GluVal: 4.357 ± 0.38
0.792GluTrp: 0.792 ± 0.172
2.575GluTyr: 2.575 ± 0.29
0.0GluXaa: 0.0 ± 0.0
Phe
2.535PheAla: 2.535 ± 0.363
0.594PheCys: 0.594 ± 0.179
3.327PheAsp: 3.327 ± 0.33
3.327PheGlu: 3.327 ± 0.387
1.228PhePhe: 1.228 ± 0.222
3.09PheGly: 3.09 ± 0.341
0.792PheHis: 0.792 ± 0.214
2.535PheIle: 2.535 ± 0.312
3.684PheLys: 3.684 ± 0.403
2.456PheLeu: 2.456 ± 0.315
1.268PheMet: 1.268 ± 0.23
2.535PheAsn: 2.535 ± 0.284
1.03PhePro: 1.03 ± 0.22
1.426PheGln: 1.426 ± 0.246
1.426PheArg: 1.426 ± 0.221
3.09PheSer: 3.09 ± 0.356
2.654PheThr: 2.654 ± 0.298
2.694PheVal: 2.694 ± 0.291
0.515PheTrp: 0.515 ± 0.123
1.941PheTyr: 1.941 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
4.793GlyAla: 4.793 ± 0.596
1.188GlyCys: 1.188 ± 0.206
3.565GlyAsp: 3.565 ± 0.353
4.12GlyGlu: 4.12 ± 0.459
3.169GlyPhe: 3.169 ± 0.359
4.555GlyGly: 4.555 ± 0.632
1.347GlyHis: 1.347 ± 0.195
4.437GlyIle: 4.437 ± 0.398
6.219GlyLys: 6.219 ± 0.493
4.555GlyLeu: 4.555 ± 0.436
1.149GlyMet: 1.149 ± 0.221
3.09GlyAsn: 3.09 ± 0.404
0.119GlyPro: 0.119 ± 0.087
2.179GlyGln: 2.179 ± 0.352
2.614GlyArg: 2.614 ± 0.316
4.08GlySer: 4.08 ± 0.522
4.159GlyThr: 4.159 ± 0.56
5.625GlyVal: 5.625 ± 0.434
0.951GlyTrp: 0.951 ± 0.197
2.812GlyTyr: 2.812 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
0.99HisAla: 0.99 ± 0.19
0.436HisCys: 0.436 ± 0.136
1.149HisAsp: 1.149 ± 0.214
1.347HisGlu: 1.347 ± 0.247
0.871HisPhe: 0.871 ± 0.202
0.832HisGly: 0.832 ± 0.161
0.475HisHis: 0.475 ± 0.192
1.149HisIle: 1.149 ± 0.221
1.268HisLys: 1.268 ± 0.25
1.664HisLeu: 1.664 ± 0.247
0.594HisMet: 0.594 ± 0.14
0.911HisAsn: 0.911 ± 0.23
0.753HisPro: 0.753 ± 0.18
0.436HisGln: 0.436 ± 0.115
0.951HisArg: 0.951 ± 0.173
1.743HisSer: 1.743 ± 0.404
1.664HisThr: 1.664 ± 0.365
1.426HisVal: 1.426 ± 0.243
0.238HisTrp: 0.238 ± 0.126
0.951HisTyr: 0.951 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
3.961IleAla: 3.961 ± 0.363
1.07IleCys: 1.07 ± 0.237
4.238IleAsp: 4.238 ± 0.407
4.159IleGlu: 4.159 ± 0.423
2.456IlePhe: 2.456 ± 0.306
3.367IleGly: 3.367 ± 0.378
1.347IleHis: 1.347 ± 0.235
4.001IleIle: 4.001 ± 0.434
5.506IleLys: 5.506 ± 0.519
4.04IleLeu: 4.04 ± 0.404
1.624IleMet: 1.624 ± 0.254
3.644IleAsn: 3.644 ± 0.419
1.822IlePro: 1.822 ± 0.316
1.783IleGln: 1.783 ± 0.241
2.773IleArg: 2.773 ± 0.335
4.08IleSer: 4.08 ± 0.48
3.842IleThr: 3.842 ± 0.392
3.407IleVal: 3.407 ± 0.316
0.753IleTrp: 0.753 ± 0.137
2.852IleTyr: 2.852 ± 0.321
0.0IleXaa: 0.0 ± 0.0
Lys
5.863LysAla: 5.863 ± 0.564
0.832LysCys: 0.832 ± 0.207
5.15LysAsp: 5.15 ± 0.49
6.378LysGlu: 6.378 ± 0.601
2.614LysPhe: 2.614 ± 0.309
5.268LysGly: 5.268 ± 0.482
1.545LysHis: 1.545 ± 0.301
4.555LysIle: 4.555 ± 0.356
5.942LysLys: 5.942 ± 0.629
6.378LysLeu: 6.378 ± 0.549
2.852LysMet: 2.852 ± 0.324
4.159LysAsn: 4.159 ± 0.32
2.614LysPro: 2.614 ± 0.317
2.971LysGln: 2.971 ± 0.298
3.486LysArg: 3.486 ± 0.346
4.476LysSer: 4.476 ± 0.427
5.11LysThr: 5.11 ± 0.483
6.774LysVal: 6.774 ± 0.575
0.713LysTrp: 0.713 ± 0.15
2.892LysTyr: 2.892 ± 0.435
0.0LysXaa: 0.0 ± 0.0
Leu
5.625LeuAla: 5.625 ± 0.484
0.99LeuCys: 0.99 ± 0.172
5.466LeuAsp: 5.466 ± 0.5
6.14LeuGlu: 6.14 ± 0.525
2.852LeuPhe: 2.852 ± 0.286
4.001LeuGly: 4.001 ± 0.348
1.743LeuHis: 1.743 ± 0.284
3.248LeuIle: 3.248 ± 0.321
6.813LeuLys: 6.813 ± 0.501
6.021LeuLeu: 6.021 ± 0.546
2.456LeuMet: 2.456 ± 0.349
4.12LeuAsn: 4.12 ± 0.432
3.129LeuPro: 3.129 ± 0.296
3.209LeuGln: 3.209 ± 0.412
3.842LeuArg: 3.842 ± 0.32
5.268LeuSer: 5.268 ± 0.48
4.912LeuThr: 4.912 ± 0.473
4.397LeuVal: 4.397 ± 0.449
0.832LeuTrp: 0.832 ± 0.2
2.931LeuTyr: 2.931 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
2.337MetAla: 2.337 ± 0.357
0.198MetCys: 0.198 ± 0.076
1.188MetAsp: 1.188 ± 0.226
1.862MetGlu: 1.862 ± 0.222
1.505MetPhe: 1.505 ± 0.304
1.584MetGly: 1.584 ± 0.219
0.317MetHis: 0.317 ± 0.11
1.822MetIle: 1.822 ± 0.26
2.971MetLys: 2.971 ± 0.369
2.416MetLeu: 2.416 ± 0.314
0.634MetMet: 0.634 ± 0.161
1.545MetAsn: 1.545 ± 0.262
0.673MetPro: 0.673 ± 0.177
1.584MetGln: 1.584 ± 0.282
1.743MetArg: 1.743 ± 0.275
2.337MetSer: 2.337 ± 0.266
1.981MetThr: 1.981 ± 0.274
0.99MetVal: 0.99 ± 0.182
0.277MetTrp: 0.277 ± 0.103
0.99MetTyr: 0.99 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.961AsnAla: 3.961 ± 0.444
0.753AsnCys: 0.753 ± 0.179
2.773AsnAsp: 2.773 ± 0.319
2.456AsnGlu: 2.456 ± 0.246
2.733AsnPhe: 2.733 ± 0.29
4.476AsnGly: 4.476 ± 0.466
1.149AsnHis: 1.149 ± 0.195
3.486AsnIle: 3.486 ± 0.428
3.961AsnLys: 3.961 ± 0.47
4.793AsnLeu: 4.793 ± 0.433
1.268AsnMet: 1.268 ± 0.255
3.01AsnAsn: 3.01 ± 0.396
2.099AsnPro: 2.099 ± 0.27
1.624AsnGln: 1.624 ± 0.267
2.179AsnArg: 2.179 ± 0.247
3.248AsnSer: 3.248 ± 0.346
2.931AsnThr: 2.931 ± 0.377
3.684AsnVal: 3.684 ± 0.429
0.673AsnTrp: 0.673 ± 0.155
2.416AsnTyr: 2.416 ± 0.308
0.0AsnXaa: 0.0 ± 0.0
Pro
1.783ProAla: 1.783 ± 0.304
0.436ProCys: 0.436 ± 0.124
2.179ProAsp: 2.179 ± 0.319
3.01ProGlu: 3.01 ± 0.37
1.624ProPhe: 1.624 ± 0.286
0.198ProGly: 0.198 ± 0.089
0.673ProHis: 0.673 ± 0.188
1.347ProIle: 1.347 ± 0.212
2.258ProLys: 2.258 ± 0.306
2.06ProLeu: 2.06 ± 0.302
1.109ProMet: 1.109 ± 0.235
1.426ProAsn: 1.426 ± 0.262
0.951ProPro: 0.951 ± 0.217
1.03ProGln: 1.03 ± 0.205
0.99ProArg: 0.99 ± 0.226
2.297ProSer: 2.297 ± 0.342
2.179ProThr: 2.179 ± 0.298
2.297ProVal: 2.297 ± 0.32
0.158ProTrp: 0.158 ± 0.067
1.268ProTyr: 1.268 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
2.337GlnAla: 2.337 ± 0.311
0.436GlnCys: 0.436 ± 0.143
1.624GlnAsp: 1.624 ± 0.26
2.179GlnGlu: 2.179 ± 0.323
1.505GlnPhe: 1.505 ± 0.246
1.743GlnGly: 1.743 ± 0.266
0.515GlnHis: 0.515 ± 0.146
2.575GlnIle: 2.575 ± 0.302
2.456GlnLys: 2.456 ± 0.346
2.733GlnLeu: 2.733 ± 0.35
0.99GlnMet: 0.99 ± 0.238
1.743GlnAsn: 1.743 ± 0.256
1.109GlnPro: 1.109 ± 0.196
1.426GlnGln: 1.426 ± 0.215
1.822GlnArg: 1.822 ± 0.287
1.901GlnSer: 1.901 ± 0.275
1.664GlnThr: 1.664 ± 0.211
2.852GlnVal: 2.852 ± 0.362
0.555GlnTrp: 0.555 ± 0.146
1.664GlnTyr: 1.664 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
2.218ArgAla: 2.218 ± 0.31
0.832ArgCys: 0.832 ± 0.205
3.09ArgAsp: 3.09 ± 0.385
2.773ArgGlu: 2.773 ± 0.328
1.386ArgPhe: 1.386 ± 0.269
2.694ArgGly: 2.694 ± 0.358
0.871ArgHis: 0.871 ± 0.205
2.575ArgIle: 2.575 ± 0.267
3.05ArgLys: 3.05 ± 0.363
3.446ArgLeu: 3.446 ± 0.418
1.188ArgMet: 1.188 ± 0.213
2.337ArgAsn: 2.337 ± 0.284
1.109ArgPro: 1.109 ± 0.205
1.466ArgGln: 1.466 ± 0.203
2.06ArgArg: 2.06 ± 0.277
2.099ArgSer: 2.099 ± 0.264
2.377ArgThr: 2.377 ± 0.327
3.129ArgVal: 3.129 ± 0.382
0.594ArgTrp: 0.594 ± 0.16
2.377ArgTyr: 2.377 ± 0.289
0.0ArgXaa: 0.0 ± 0.0
Ser
4.001SerAla: 4.001 ± 0.536
0.634SerCys: 0.634 ± 0.186
4.278SerAsp: 4.278 ± 0.44
4.635SerGlu: 4.635 ± 0.412
3.05SerPhe: 3.05 ± 0.344
3.803SerGly: 3.803 ± 0.471
1.07SerHis: 1.07 ± 0.221
3.922SerIle: 3.922 ± 0.392
4.714SerLys: 4.714 ± 0.432
5.664SerLeu: 5.664 ± 0.445
1.584SerMet: 1.584 ± 0.232
3.486SerAsn: 3.486 ± 0.404
1.743SerPro: 1.743 ± 0.309
2.297SerGln: 2.297 ± 0.334
2.535SerArg: 2.535 ± 0.314
4.555SerSer: 4.555 ± 0.457
2.971SerThr: 2.971 ± 0.391
5.268SerVal: 5.268 ± 0.429
1.03SerTrp: 1.03 ± 0.172
2.892SerTyr: 2.892 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
4.278ThrAla: 4.278 ± 0.463
0.515ThrCys: 0.515 ± 0.126
3.724ThrAsp: 3.724 ± 0.366
3.446ThrGlu: 3.446 ± 0.368
2.931ThrPhe: 2.931 ± 0.346
5.268ThrGly: 5.268 ± 0.5
1.624ThrHis: 1.624 ± 0.293
3.882ThrIle: 3.882 ± 0.395
4.674ThrLys: 4.674 ± 0.514
4.555ThrLeu: 4.555 ± 0.427
0.871ThrMet: 0.871 ± 0.183
2.694ThrAsn: 2.694 ± 0.45
2.099ThrPro: 2.099 ± 0.266
2.337ThrGln: 2.337 ± 0.271
2.496ThrArg: 2.496 ± 0.268
3.684ThrSer: 3.684 ± 0.47
3.605ThrThr: 3.605 ± 0.554
5.348ThrVal: 5.348 ± 0.508
0.594ThrTrp: 0.594 ± 0.153
2.852ThrTyr: 2.852 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
5.981ValAla: 5.981 ± 0.498
1.149ValCys: 1.149 ± 0.264
4.714ValAsp: 4.714 ± 0.417
4.238ValGlu: 4.238 ± 0.5
2.456ValPhe: 2.456 ± 0.333
4.476ValGly: 4.476 ± 0.521
1.268ValHis: 1.268 ± 0.244
4.357ValIle: 4.357 ± 0.512
6.259ValLys: 6.259 ± 0.48
4.397ValLeu: 4.397 ± 0.433
2.337ValMet: 2.337 ± 0.295
4.04ValAsn: 4.04 ± 0.395
2.575ValPro: 2.575 ± 0.343
1.981ValGln: 1.981 ± 0.285
2.694ValArg: 2.694 ± 0.302
4.714ValSer: 4.714 ± 0.415
5.15ValThr: 5.15 ± 0.499
4.357ValVal: 4.357 ± 0.42
0.634ValTrp: 0.634 ± 0.192
2.852ValTyr: 2.852 ± 0.362
0.0ValXaa: 0.0 ± 0.0
Trp
0.475TrpAla: 0.475 ± 0.142
0.119TrpCys: 0.119 ± 0.064
1.07TrpAsp: 1.07 ± 0.179
0.951TrpGlu: 0.951 ± 0.186
0.634TrpPhe: 0.634 ± 0.157
0.634TrpGly: 0.634 ± 0.152
0.04TrpHis: 0.04 ± 0.045
0.634TrpIle: 0.634 ± 0.191
1.07TrpLys: 1.07 ± 0.189
1.03TrpLeu: 1.03 ± 0.217
0.475TrpMet: 0.475 ± 0.16
0.871TrpAsn: 0.871 ± 0.199
0.238TrpPro: 0.238 ± 0.071
0.436TrpGln: 0.436 ± 0.137
0.396TrpArg: 0.396 ± 0.156
0.753TrpSer: 0.753 ± 0.168
0.634TrpThr: 0.634 ± 0.146
0.792TrpVal: 0.792 ± 0.161
0.158TrpTrp: 0.158 ± 0.073
0.673TrpTyr: 0.673 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.733TyrAla: 2.733 ± 0.368
0.911TyrCys: 0.911 ± 0.166
2.575TyrAsp: 2.575 ± 0.333
2.812TyrGlu: 2.812 ± 0.315
1.466TyrPhe: 1.466 ± 0.239
3.169TyrGly: 3.169 ± 0.391
0.911TyrHis: 0.911 ± 0.194
2.456TyrIle: 2.456 ± 0.29
3.209TyrLys: 3.209 ± 0.347
3.644TyrLeu: 3.644 ± 0.337
1.07TyrMet: 1.07 ± 0.187
2.179TyrAsn: 2.179 ± 0.289
1.703TyrPro: 1.703 ± 0.241
1.624TyrGln: 1.624 ± 0.29
1.664TyrArg: 1.664 ± 0.27
2.694TyrSer: 2.694 ± 0.355
3.644TyrThr: 3.644 ± 0.376
2.535TyrVal: 2.535 ± 0.329
0.357TyrTrp: 0.357 ± 0.109
2.06TyrTyr: 2.06 ± 0.323
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 126 proteins (25246 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski