Dorea sp. AF36-15AT

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Dorea; unclassified Dorea

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2785 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A396L2J9|A0A396L2J9_9FIRM Histidine kinase OS=Dorea sp. AF36-15AT OX=2292041 GN=DWZ93_06190 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 10.64NRR4 pKa = 11.84ITRR7 pKa = 11.84IAMTLTMLMVPALVTGCGPSKK28 pKa = 10.65KK29 pKa = 10.0QIQQEE34 pKa = 4.01LSYY37 pKa = 10.94QEE39 pKa = 4.28PDD41 pKa = 3.09QEE43 pKa = 4.34EE44 pKa = 4.1LHH46 pKa = 7.0EE47 pKa = 4.69EE48 pKa = 4.05YY49 pKa = 10.64QYY51 pKa = 11.32LAEE54 pKa = 4.22VNEE57 pKa = 4.42GDD59 pKa = 4.07GVATGLINEE68 pKa = 4.33YY69 pKa = 10.08GEE71 pKa = 4.72CVVACMFDD79 pKa = 4.42EE80 pKa = 4.33ITMGMTDD87 pKa = 3.52DD88 pKa = 4.19GFRR91 pKa = 11.84ILAYY95 pKa = 9.89KK96 pKa = 10.01SVYY99 pKa = 8.65TDD101 pKa = 3.16KK102 pKa = 11.15QGEE105 pKa = 4.43SEE107 pKa = 4.14AYY109 pKa = 9.28RR110 pKa = 11.84YY111 pKa = 9.86YY112 pKa = 11.27VLDD115 pKa = 3.34EE116 pKa = 4.52RR117 pKa = 11.84GQILEE122 pKa = 3.96EE123 pKa = 4.75HH124 pKa = 6.66GEE126 pKa = 4.39GIEE129 pKa = 4.09EE130 pKa = 4.16EE131 pKa = 4.3GGGYY135 pKa = 10.14YY136 pKa = 10.46DD137 pKa = 5.37GEE139 pKa = 4.39ALLEE143 pKa = 4.28EE144 pKa = 4.61MDD146 pKa = 3.79NYY148 pKa = 10.83TPVEE152 pKa = 4.16VEE154 pKa = 4.02DD155 pKa = 4.1AVSEE159 pKa = 4.12EE160 pKa = 4.39AEE162 pKa = 4.28EE163 pKa = 4.13EE164 pKa = 4.06TAEE167 pKa = 4.36ADD169 pKa = 3.66SAPTDD174 pKa = 3.72VEE176 pKa = 4.71IVTTDD181 pKa = 3.25DD182 pKa = 3.28GDD184 pKa = 4.11YY185 pKa = 11.36EE186 pKa = 5.36LVDD189 pKa = 3.32QSGKK193 pKa = 10.21VLISDD198 pKa = 3.82EE199 pKa = 4.61DD200 pKa = 4.02VSGMGVSDD208 pKa = 4.87IISMYY213 pKa = 8.23FTRR216 pKa = 11.84DD217 pKa = 2.97GKK219 pKa = 10.74YY220 pKa = 10.47VIADD224 pKa = 4.03TGSMLYY230 pKa = 10.59DD231 pKa = 3.89EE232 pKa = 6.07DD233 pKa = 4.16EE234 pKa = 4.55LPIGGNVLFDD244 pKa = 3.91LEE246 pKa = 4.87GNVKK250 pKa = 10.32RR251 pKa = 11.84SNDD254 pKa = 3.68YY255 pKa = 11.09EE256 pKa = 4.59SISYY260 pKa = 10.77GGDD263 pKa = 3.3NGWLCVHH270 pKa = 6.43NKK272 pKa = 8.08KK273 pKa = 8.77TNQTVYY279 pKa = 10.9LNSDD283 pKa = 4.17LKK285 pKa = 10.51TEE287 pKa = 4.59LDD289 pKa = 3.56LGDD292 pKa = 4.34AYY294 pKa = 10.66GAAGDD299 pKa = 3.89FKK301 pKa = 11.19KK302 pKa = 10.66CLRR305 pKa = 11.84RR306 pKa = 11.84QQ307 pKa = 3.21

Molecular weight: 34.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A396L5D0|A0A396L5D0_9FIRM ImmA/IrrE family metallo-endopeptidase OS=Dorea sp. AF36-15AT OX=2292041 GN=DWZ93_11500 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.87VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2785

0

2785

871764

27

3528

313.0

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.565 ± 0.05

1.533 ± 0.021

5.833 ± 0.037

7.849 ± 0.059

3.751 ± 0.036

7.218 ± 0.041

1.856 ± 0.02

7.31 ± 0.044

6.877 ± 0.046

8.694 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.306 ± 0.026

4.154 ± 0.031

3.262 ± 0.027

3.295 ± 0.028

4.47 ± 0.044

5.473 ± 0.032

5.469 ± 0.048

7.122 ± 0.042

0.861 ± 0.018

4.103 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski