Actinomadura sp. KC345

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4RDG1|A0A4R4RDG1_9ACTN Uncharacterized protein OS=Actinomadura sp. KC345 OX=2530371 GN=E1281_26690 PE=4 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84FRR5 pKa = 11.84QVVVLGTAAAAAFALTASPASAATTTIRR33 pKa = 11.84AGSADD38 pKa = 3.5AAPYY42 pKa = 9.78SGNVQASLLGEE53 pKa = 4.17ATVEE57 pKa = 3.86ASIGSGSCSEE67 pKa = 4.11STMTGSINSDD77 pKa = 3.06GTGLSISGAGFSEE90 pKa = 4.61CTGTAGVTITAMALPWTGGAVAFDD114 pKa = 3.82PAGTNGRR121 pKa = 11.84DD122 pKa = 3.43ATVTIANFSVEE133 pKa = 4.09AVVDD137 pKa = 3.98LFGGITCNFGGTLTADD153 pKa = 4.72GYY155 pKa = 11.67NGDD158 pKa = 3.75NASRR162 pKa = 11.84PVADD166 pKa = 3.62NAQAQVGVNDD176 pKa = 3.59ATVNRR181 pKa = 11.84QSGSNFLCPSTAQISATYY199 pKa = 9.16EE200 pKa = 3.95LLGEE204 pKa = 4.46TSAGSGTFDD213 pKa = 2.92QSLYY217 pKa = 9.03VTGSAA222 pKa = 4.83

Molecular weight: 21.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4RE46|A0A4R4RE46_9ACTN Gluconokinase OS=Actinomadura sp. KC345 OX=2530371 GN=E1281_27490 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILTNRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.95GRR40 pKa = 11.84SRR42 pKa = 11.84IAVV45 pKa = 3.34

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7614

0

7614

2370126

29

3998

311.3

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.321 ± 0.039

0.79 ± 0.009

6.173 ± 0.023

5.957 ± 0.029

2.806 ± 0.015

9.806 ± 0.028

2.218 ± 0.014

3.471 ± 0.019

1.997 ± 0.022

10.207 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.011

1.684 ± 0.016

6.15 ± 0.025

2.451 ± 0.017

8.606 ± 0.03

4.808 ± 0.016

5.603 ± 0.02

8.524 ± 0.024

1.495 ± 0.012

1.988 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski