Streptococcus phage Javan190

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AV76|A0A4D6AV76_9CAUD Uncharacterized protein OS=Streptococcus phage Javan190 OX=2548038 GN=Javan190_0034 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.15NMLEE7 pKa = 3.97EE8 pKa = 4.63LEE10 pKa = 4.43NEE12 pKa = 3.94ILEE15 pKa = 4.79DD16 pKa = 3.87DD17 pKa = 3.74QCICEE22 pKa = 4.16SSNLTGYY29 pKa = 10.39SDD31 pKa = 3.79WYY33 pKa = 10.39KK34 pKa = 11.24KK35 pKa = 10.55NADD38 pKa = 3.86SKK40 pKa = 10.6VWWIDD45 pKa = 3.3EE46 pKa = 4.0LDD48 pKa = 3.31VRR50 pKa = 11.84GRR52 pKa = 11.84HH53 pKa = 5.4LFSFDD58 pKa = 2.44RR59 pKa = 11.84HH60 pKa = 5.82KK61 pKa = 10.67IYY63 pKa = 11.05NLFADD68 pKa = 4.75YY69 pKa = 9.74PHH71 pKa = 6.85NMTDD75 pKa = 3.76DD76 pKa = 3.54EE77 pKa = 5.1VKK79 pKa = 10.67IFDD82 pKa = 4.19NEE84 pKa = 3.74EE85 pKa = 4.03KK86 pKa = 10.79YY87 pKa = 9.48WADD90 pKa = 3.26FLKK93 pKa = 10.77SRR95 pKa = 11.84KK96 pKa = 7.31QQ97 pKa = 3.21

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AV66|A0A4D6AV66_9CAUD Uncharacterized protein OS=Streptococcus phage Javan190 OX=2548038 GN=Javan190_0024 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.43LFNWIFAKK10 pKa = 10.53PKK12 pKa = 7.38TQQLIKK18 pKa = 10.35HH19 pKa = 5.68DD20 pKa = 3.95TLRR23 pKa = 11.84ASSEE27 pKa = 4.18QQWARR32 pKa = 11.84FDD34 pKa = 5.03AYY36 pKa = 10.24MKK38 pKa = 8.92QQCKK42 pKa = 8.47RR43 pKa = 3.38

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10383

37

786

192.3

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.281 ± 0.526

0.665 ± 0.112

6.491 ± 0.261

6.694 ± 0.43

3.727 ± 0.241

6.578 ± 0.511

1.599 ± 0.164

7.426 ± 0.262

8.735 ± 0.375

8.755 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.175

5.432 ± 0.266

2.764 ± 0.271

4.267 ± 0.216

4.064 ± 0.311

6.838 ± 0.354

5.827 ± 0.3

6.058 ± 0.313

0.982 ± 0.109

3.496 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski