Kurlavirus BKC-1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Marseillevirus; unclassified Marseillevirus

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 386 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5XYX3|A0A1S5XYX3_9VIRU Putative glycosyltransferase OS=Kurlavirus BKC-1 OX=1958810 PE=4 SV=1
MM1 pKa = 7.49SRR3 pKa = 11.84LQGFFKK9 pKa = 10.51DD10 pKa = 3.47IPIVGSYY17 pKa = 10.72SVLPPVLPRR26 pKa = 11.84GSLVFLTSDD35 pKa = 3.2GNLYY39 pKa = 10.37VSNGEE44 pKa = 4.0TWNATGGDD52 pKa = 3.95IGPLAAQVAQNTADD66 pKa = 3.29IGVLQTDD73 pKa = 3.92VSTLQGEE80 pKa = 4.82VATNTLDD87 pKa = 3.32IGTLQTQVATNTSDD101 pKa = 2.88IGTLQTQVAGNSTNISALQTDD122 pKa = 4.27VSTLQGQVATNTTDD136 pKa = 2.67IGTLQSQVATNTTNIQQNSNDD157 pKa = 2.97ITNIIVAQSQGFTIADD173 pKa = 3.47NAQLRR178 pKa = 11.84TYY180 pKa = 8.03TQTIARR186 pKa = 11.84VDD188 pKa = 3.72VASTSPTVIYY198 pKa = 9.38TLNIPLTSNINAVYY212 pKa = 10.05LIQWNVLAHH221 pKa = 5.23YY222 pKa = 7.54QTANVYY228 pKa = 9.95SAVGQSSVLSNGVGSLVSVFGTTTDD253 pKa = 3.5QTNSGSATIAVTSDD267 pKa = 2.97FSTPVFRR274 pKa = 11.84LIVTSDD280 pKa = 3.23SAVITTYY287 pKa = 10.32SGYY290 pKa = 11.17VIVTNILNISPP301 pKa = 4.0

Molecular weight: 31.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5XYL0|A0A1S5XYL0_9VIRU DUF5858 domain-containing protein OS=Kurlavirus BKC-1 OX=1958810 PE=4 SV=1
MM1 pKa = 6.64QQRR4 pKa = 11.84LVNPEE9 pKa = 3.62TGRR12 pKa = 11.84NILYY16 pKa = 10.34GGKK19 pKa = 7.29THH21 pKa = 7.2LNLVRR26 pKa = 11.84RR27 pKa = 11.84GILPKK32 pKa = 10.57EE33 pKa = 3.98EE34 pKa = 4.08PAKK37 pKa = 10.67KK38 pKa = 10.17GAGGSNVKK46 pKa = 10.07KK47 pKa = 10.51YY48 pKa = 10.62KK49 pKa = 10.1KK50 pKa = 9.2EE51 pKa = 3.8HH52 pKa = 6.36LPASDD57 pKa = 3.38FCGTVPGSFPVNTEE71 pKa = 3.4RR72 pKa = 11.84RR73 pKa = 11.84AKK75 pKa = 10.48AALSYY80 pKa = 10.95ARR82 pKa = 11.84NDD84 pKa = 3.41VNPEE88 pKa = 3.76RR89 pKa = 11.84VRR91 pKa = 11.84EE92 pKa = 4.01CARR95 pKa = 11.84RR96 pKa = 11.84KK97 pKa = 8.83AKK99 pKa = 10.52RR100 pKa = 11.84MGWFF104 pKa = 3.21

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

386

0

386

104158

99

1525

269.8

30.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.201 ± 0.12

2.567 ± 0.115

4.854 ± 0.078

8.378 ± 0.185

5.685 ± 0.11

6.194 ± 0.118

1.768 ± 0.058

5.038 ± 0.075

8.866 ± 0.179

8.658 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.953 ± 0.055

4.195 ± 0.099

3.933 ± 0.098

3.392 ± 0.1

5.318 ± 0.097

7.569 ± 0.153

5.31 ± 0.149

6.431 ± 0.092

1.495 ± 0.048

3.195 ± 0.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski