Bacillus phage 000TH010

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PHR5|A0A5P8PHR5_9CAUD Uncharacterized protein OS=Bacillus phage 000TH010 OX=2601652 GN=43 PE=4 SV=1
MM1 pKa = 7.55SEE3 pKa = 4.13SLLYY7 pKa = 10.65GYY9 pKa = 10.64FLDD12 pKa = 3.75SWLDD16 pKa = 3.53GTASEE21 pKa = 4.22EE22 pKa = 4.07LLRR25 pKa = 11.84VAVSCGDD32 pKa = 3.65LTQEE36 pKa = 4.12EE37 pKa = 4.47ADD39 pKa = 4.75KK40 pKa = 11.23IMSYY44 pKa = 10.34PWGAWNEE51 pKa = 3.93

Molecular weight: 5.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PHR1|A0A5P8PHR1_9CAUD Beta_helix domain-containing protein OS=Bacillus phage 000TH010 OX=2601652 GN=49 PE=4 SV=1
MM1 pKa = 7.61SEE3 pKa = 4.15KK4 pKa = 10.58NRR6 pKa = 11.84FNKK9 pKa = 9.5PVAFNKK15 pKa = 10.14KK16 pKa = 9.78NADD19 pKa = 3.37DD20 pKa = 3.87QLILKK25 pKa = 9.45HH26 pKa = 4.99VSRR29 pKa = 11.84RR30 pKa = 11.84NFSGYY35 pKa = 8.23VKK37 pKa = 10.63KK38 pKa = 10.94LILADD43 pKa = 3.18IEE45 pKa = 4.44SRR47 pKa = 11.84KK48 pKa = 9.79DD49 pKa = 3.35LKK51 pKa = 10.57PVRR54 pKa = 11.84EE55 pKa = 4.07AEE57 pKa = 4.34KK58 pKa = 10.61VKK60 pKa = 9.59KK61 pKa = 8.05TASSRR66 pKa = 11.84MEE68 pKa = 4.09EE69 pKa = 3.7MRR71 pKa = 11.84KK72 pKa = 8.95RR73 pKa = 11.84AKK75 pKa = 10.01PSRR78 pKa = 11.84RR79 pKa = 11.84PPGGVFTPTHH89 pKa = 4.55QQYY92 pKa = 9.89NQQ94 pKa = 2.94

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

15047

31

1115

167.2

18.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.497 ± 0.558

0.851 ± 0.127

5.981 ± 0.33

8.42 ± 0.494

3.894 ± 0.152

7.184 ± 0.298

1.887 ± 0.154

6.679 ± 0.218

8.473 ± 0.48

7.423 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.971 ± 0.125

5.396 ± 0.305

3.31 ± 0.196

3.323 ± 0.188

4.685 ± 0.22

5.337 ± 0.322

5.503 ± 0.291

6.101 ± 0.229

1.329 ± 0.111

3.755 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski