Cystoviridae sp.
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G5YMP6|A0A6G5YMP6_9VIRU Uncharacterized protein OS=Cystoviridae sp. OX=2584979 PE=4 SV=1
MM1 pKa = 7.19 EE2 pKa = 6.11 HH3 pKa = 4.98 YY4 pKa = 9.7 TIYY7 pKa = 10.65 GKK9 pKa = 8.64 EE10 pKa = 3.75 ACPFCDD16 pKa = 3.0 NSKK19 pKa = 11.0 AILSQRR25 pKa = 11.84 EE26 pKa = 3.32 KK27 pKa = 11.24 DD28 pKa = 3.43 FTYY31 pKa = 10.77 NQLDD35 pKa = 3.1 IHH37 pKa = 5.28 YY38 pKa = 8.62 TRR40 pKa = 11.84 EE41 pKa = 3.66 EE42 pKa = 3.92 FFDD45 pKa = 4.25 FFASKK50 pKa = 10.6 FDD52 pKa = 3.6 VVPRR56 pKa = 11.84 TFPQIILTTDD66 pKa = 2.49 IGDD69 pKa = 3.71 TYY71 pKa = 11.35 LGGFTEE77 pKa = 5.62 LRR79 pKa = 11.84 EE80 pKa = 4.14 HH81 pKa = 7.05 LNNN84 pKa = 3.93
Molecular weight: 9.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.67
IPC2_protein 4.838
IPC_protein 4.698
Toseland 4.584
ProMoST 4.825
Dawson 4.673
Bjellqvist 4.813
Wikipedia 4.558
Rodwell 4.571
Grimsley 4.495
Solomon 4.673
Lehninger 4.622
Nozaki 4.787
DTASelect 4.952
Thurlkill 4.596
EMBOSS 4.584
Sillero 4.838
Patrickios 3.389
IPC_peptide 4.673
IPC2_peptide 4.838
IPC2.peptide.svr19 4.781
Protein with the highest isoelectric point:
>tr|A0A6G5YMF4|A0A6G5YMF4_9VIRU Uncharacterized protein OS=Cystoviridae sp. OX=2584979 PE=4 SV=1
MM1 pKa = 7.55 NYY3 pKa = 10.3 KK4 pKa = 10.34 SGEE7 pKa = 3.95 QYY9 pKa = 10.96 KK10 pKa = 10.26 VIRR13 pKa = 11.84 RR14 pKa = 11.84 NGNKK18 pKa = 9.15 EE19 pKa = 3.84 VATLTITEE27 pKa = 4.09 GHH29 pKa = 6.38 CEE31 pKa = 3.69 KK32 pKa = 10.75 IMARR36 pKa = 11.84 KK37 pKa = 9.56 IFFLYY42 pKa = 8.99 GLKK45 pKa = 9.95 MNEE48 pKa = 3.24 IRR50 pKa = 11.84 LIKK53 pKa = 10.37 INN55 pKa = 3.57
Molecular weight: 6.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.081
IPC2_protein 9.282
IPC_protein 9.268
Toseland 10.014
ProMoST 9.648
Dawson 10.189
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.716
Grimsley 10.248
Solomon 10.233
Lehninger 10.204
Nozaki 10.014
DTASelect 9.823
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.101
Patrickios 10.496
IPC_peptide 10.233
IPC2_peptide 8.463
IPC2.peptide.svr19 8.232
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
3223
35
900
214.9
24.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.964 ± 0.801
2.141 ± 0.413
5.523 ± 0.548
8.222 ± 0.348
4.561 ± 0.53
6.392 ± 0.443
2.606 ± 0.229
6.33 ± 0.238
8.377 ± 0.907
7.353 ± 0.449
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.42 ± 0.224
5.802 ± 0.379
3.01 ± 0.397
3.444 ± 0.484
4.282 ± 0.485
6.392 ± 0.348
5.368 ± 0.431
6.33 ± 0.447
1.738 ± 0.262
4.747 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here