Ajellomyces capsulatus (strain H143) (Darling s disease fungus) (Histoplasma capsulatum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6HAB4|C6HAB4_AJECH RNA binding protein OS=Ajellomyces capsulatus (strain H143) OX=544712 GN=HCDG_03145 PE=4 SV=1
MM1 pKa = 6.88QWVLHH6 pKa = 6.39RR7 pKa = 11.84LTALSGGADD16 pKa = 3.59VLEE19 pKa = 5.23LDD21 pKa = 5.52LAPDD25 pKa = 4.8DD26 pKa = 4.52NDD28 pKa = 4.12SDD30 pKa = 4.25GSSHH34 pKa = 7.14LNISDD39 pKa = 3.73EE40 pKa = 4.47TPLTEE45 pKa = 4.42GVTLKK50 pKa = 10.63EE51 pKa = 3.89YY52 pKa = 10.92LDD54 pKa = 3.66TYY56 pKa = 10.73EE57 pKa = 4.43YY58 pKa = 11.23GSTEE62 pKa = 4.63INDD65 pKa = 2.79GWYY68 pKa = 9.64RR69 pKa = 11.84SQIIGSS75 pKa = 3.74

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6HK97|C6HK97_AJECH Uncharacterized protein OS=Ajellomyces capsulatus (strain H143) OX=544712 GN=HCDG_06628 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 10.07SQQWTPSIHH11 pKa = 5.29STTRR15 pKa = 11.84SLANRR20 pKa = 11.84HH21 pKa = 5.9SGAILQEE28 pKa = 4.41SGPVPLPPRR37 pKa = 11.84LYY39 pKa = 11.0GPGSLPTTGRR49 pKa = 11.84SQGTYY54 pKa = 10.14DD55 pKa = 3.39PLGRR59 pKa = 11.84RR60 pKa = 11.84EE61 pKa = 4.29NEE63 pKa = 4.0LSPAAPSGPPPSSLIYY79 pKa = 9.79PPQAYY84 pKa = 8.17HH85 pKa = 7.21HH86 pKa = 6.62EE87 pKa = 4.79IIRR90 pKa = 11.84HH91 pKa = 5.8AKK93 pKa = 9.21PSPPPGPFASMHH105 pKa = 6.46PEE107 pKa = 3.87PTPSFHH113 pKa = 7.02SRR115 pKa = 11.84HH116 pKa = 5.6SSRR119 pKa = 11.84GSVIKK124 pKa = 10.64GGEE127 pKa = 4.29GVKK130 pKa = 10.64EE131 pKa = 4.14PGNALYY137 pKa = 10.72RR138 pKa = 11.84EE139 pKa = 4.25DD140 pKa = 3.6TNIFVGVFLEE150 pKa = 4.3PWYY153 pKa = 10.77SGRR156 pKa = 11.84DD157 pKa = 3.46SNKK160 pKa = 8.3PAKK163 pKa = 10.09LRR165 pKa = 11.84ACIEE169 pKa = 4.16TLLCLSPRR177 pKa = 11.84PTHH180 pKa = 6.81HH181 pKa = 6.44EE182 pKa = 3.74QTEE185 pKa = 4.09PSSNRR190 pKa = 11.84LATKK194 pKa = 10.03QAAKK198 pKa = 10.23FPSPQVSLTQPPHH211 pKa = 5.7VKK213 pKa = 10.2LIHH216 pKa = 6.08HH217 pKa = 6.74HH218 pKa = 5.66LHH220 pKa = 6.67RR221 pKa = 11.84PPCRR225 pKa = 11.84RR226 pKa = 11.84GTCRR230 pKa = 11.84IPRR233 pKa = 11.84SPTSRR238 pKa = 11.84QGDD241 pKa = 4.25GLWAAMTVLLSILPQGKK258 pKa = 8.24LTTPAFSNRR267 pKa = 11.84LHH269 pKa = 7.11PLRR272 pKa = 11.84PTIVARR278 pKa = 11.84CLLHH282 pKa = 6.77LLPSTTTRR290 pKa = 11.84VEE292 pKa = 4.48APFQPPLLPNRR303 pKa = 11.84PPSLHH308 pKa = 6.36VNPPNHH314 pKa = 6.52PHH316 pKa = 5.62TARR319 pKa = 11.84VAACRR324 pKa = 11.84YY325 pKa = 6.99RR326 pKa = 11.84QCC328 pKa = 4.73

Molecular weight: 36.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9545

0

9545

3861477

39

5826

404.6

44.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.909 ± 0.021

1.288 ± 0.009

5.542 ± 0.018

6.135 ± 0.025

3.649 ± 0.017

6.716 ± 0.025

2.511 ± 0.011

5.119 ± 0.02

5.011 ± 0.021

8.897 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.01

3.953 ± 0.015

6.236 ± 0.027

4.048 ± 0.019

6.463 ± 0.022

8.829 ± 0.033

5.852 ± 0.017

5.784 ± 0.017

1.335 ± 0.009

2.617 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski