Human parvovirus B19 (strain HV) (HPV B19)
Average proteome isoelectric point is 7.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9PZT0-2|CAPSD-2_PAVHV Isoform of Q9PZT0 Isoform Major capsid protein VP2 of Minor capsid protein VP1 OS=Human parvovirus B19 (strain HV) OX=648237 GN=vp PE=1 SV=1
MM1 pKa = 7.42 EE2 pKa = 5.69 LFRR5 pKa = 11.84 GVLQVSSNVLDD16 pKa = 4.09 CANDD20 pKa = 3.54 NWWCSLLDD28 pKa = 4.52 LDD30 pKa = 4.58 TSDD33 pKa = 4.51 WEE35 pKa = 4.51 PLTHH39 pKa = 6.33 TNRR42 pKa = 11.84 LMAIYY47 pKa = 9.89 LSSVASKK54 pKa = 11.22 LDD56 pKa = 3.69 FTGGPLAGCLYY67 pKa = 10.0 FFQVEE72 pKa = 4.38 CNKK75 pKa = 10.02 FEE77 pKa = 4.32 EE78 pKa = 6.0 GYY80 pKa = 10.13 HH81 pKa = 4.95 IHH83 pKa = 6.23 VVIGGPGLNPRR94 pKa = 11.84 NLTVCVEE101 pKa = 4.15 GLFNNVLYY109 pKa = 10.84 HH110 pKa = 7.15 LVTEE114 pKa = 4.21 NVKK117 pKa = 10.88 LKK119 pKa = 9.87 FLPGMTTKK127 pKa = 10.67 GKK129 pKa = 9.46 YY130 pKa = 9.84 FRR132 pKa = 11.84 DD133 pKa = 3.76 GEE135 pKa = 4.32 QFIEE139 pKa = 4.17 NYY141 pKa = 9.96 LMKK144 pKa = 10.45 KK145 pKa = 9.83 IPLNVVWCVTNIDD158 pKa = 4.51 GYY160 pKa = 10.72 IDD162 pKa = 3.5 TCISATFRR170 pKa = 11.84 RR171 pKa = 11.84 GACHH175 pKa = 6.39 AKK177 pKa = 10.11 KK178 pKa = 10.17 PRR180 pKa = 11.84 ITTAINDD187 pKa = 3.84 TSSDD191 pKa = 3.41 AGEE194 pKa = 4.2 SSGTGAEE201 pKa = 3.92 VVPINGKK208 pKa = 6.92 GTKK211 pKa = 10.13 ASIKK215 pKa = 9.62 FQTMVNWLCEE225 pKa = 3.97 NRR227 pKa = 11.84 VFTEE231 pKa = 4.36 DD232 pKa = 2.61 KK233 pKa = 10.24 WKK235 pKa = 10.84 LVDD238 pKa = 4.25 FNQYY242 pKa = 8.33 TLLSSSHH249 pKa = 6.29 SGSFQIQSALKK260 pKa = 9.41 LAIYY264 pKa = 10.0 KK265 pKa = 8.2 ATNLVPTSTFLLHH278 pKa = 6.7 TDD280 pKa = 4.73 FEE282 pKa = 4.74 QVMCIKK288 pKa = 10.5 DD289 pKa = 3.64 NKK291 pKa = 9.48 IVKK294 pKa = 10.06 LLLCQNYY301 pKa = 10.3 DD302 pKa = 3.57 PLLVGQHH309 pKa = 4.24 VLKK312 pKa = 10.54 WIDD315 pKa = 3.44 KK316 pKa = 10.25 KK317 pKa = 11.02 CGKK320 pKa = 10.44 KK321 pKa = 8.43 NTLWFYY327 pKa = 10.95 GPPSTGKK334 pKa = 8.67 TNLAMAIAKK343 pKa = 8.26 SVPVYY348 pKa = 11.35 GMVNWNNEE356 pKa = 3.9 NFPFNDD362 pKa = 3.11 VAGKK366 pKa = 10.34 SLVVWDD372 pKa = 4.04 EE373 pKa = 4.53 GIIKK377 pKa = 8.56 STIVEE382 pKa = 3.9 AAKK385 pKa = 10.54 AILGGQPTRR394 pKa = 11.84 VDD396 pKa = 2.94 QKK398 pKa = 9.77 MRR400 pKa = 11.84 GSVAVPGVPVVITSNGDD417 pKa = 2.82 ITFVVSGNTTTTVHH431 pKa = 6.73 AKK433 pKa = 9.85 ALKK436 pKa = 10.0 EE437 pKa = 3.91 RR438 pKa = 11.84 MVKK441 pKa = 10.64 LNFTVRR447 pKa = 11.84 CSPDD451 pKa = 2.98 MGLLTEE457 pKa = 5.44 ADD459 pKa = 3.75 VQQWLTWCNAQSWDD473 pKa = 4.16 HH474 pKa = 5.84 YY475 pKa = 10.07 EE476 pKa = 3.7 NWAINYY482 pKa = 7.53 TFDD485 pKa = 4.51 FPGINADD492 pKa = 3.84 ALHH495 pKa = 7.51 PDD497 pKa = 3.8 LQTTPIVTDD506 pKa = 3.41 TSISSSGGEE515 pKa = 3.93 SSEE518 pKa = 4.12 EE519 pKa = 3.7 LSEE522 pKa = 4.59 SSFFNLITPGAWNTEE537 pKa = 4.41 TPRR540 pKa = 11.84 SSTPIPGTSSGEE552 pKa = 4.22 SFVGSSVSSEE562 pKa = 3.84 VVAASWEE569 pKa = 4.22 EE570 pKa = 3.88 AFYY573 pKa = 10.7 TPLADD578 pKa = 3.63 QFRR581 pKa = 11.84 EE582 pKa = 4.0 LLVGVDD588 pKa = 3.93 YY589 pKa = 11.4 VWDD592 pKa = 4.18 GVRR595 pKa = 11.84 GLPVCCVQHH604 pKa = 6.43 INNSGGGLGLCPHH617 pKa = 7.82 CINVGAWYY625 pKa = 10.11 NGWKK629 pKa = 9.97 FRR631 pKa = 11.84 EE632 pKa = 4.2 FTPDD636 pKa = 3.72 LVRR639 pKa = 11.84 CSCHH643 pKa = 6.95 VGASNPFSVLTCKK656 pKa = 10.3 KK657 pKa = 9.69 CAYY660 pKa = 10.47 LSGLQSFVDD669 pKa = 4.03 YY670 pKa = 10.7 EE671 pKa = 4.05
Molecular weight: 74.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.406
IPC2_protein 5.486
IPC_protein 5.499
Toseland 5.741
ProMoST 5.728
Dawson 5.703
Bjellqvist 5.753
Wikipedia 5.677
Rodwell 5.664
Grimsley 5.804
Solomon 5.703
Lehninger 5.69
Nozaki 5.944
DTASelect 6.122
Thurlkill 6.071
EMBOSS 6.033
Sillero 6.033
Patrickios 3.63
IPC_peptide 5.715
IPC2_peptide 6.033
IPC2.peptide.svr19 6.026
Protein with the highest isoelectric point:
>sp|P0DJZ0|11K_PAVHV Host-modulation protein 11K OS=Human parvovirus B19 (strain HV) OX=648237 GN=11K PE=1 SV=1
MM1 pKa = 7.29 QMPSTQTSKK10 pKa = 10.77 PPQLSQTPVSAAVVVKK26 pKa = 10.53 ALKK29 pKa = 10.54 NSVKK33 pKa = 10.5 AAFLTSSPQAPGTLKK48 pKa = 10.27 PRR50 pKa = 11.84 ALVRR54 pKa = 11.84 PSPGPVQEE62 pKa = 4.2 NHH64 pKa = 6.39 LSEE67 pKa = 4.77 AQFPPKK73 pKa = 10.4 LL74 pKa = 3.64
Molecular weight: 7.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.131
IPC2_protein 9.736
IPC_protein 9.823
Toseland 11.052
ProMoST 10.496
Dawson 11.096
Bjellqvist 10.657
Wikipedia 11.184
Rodwell 11.725
Grimsley 11.096
Solomon 11.169
Lehninger 11.155
Nozaki 11.008
DTASelect 10.657
Thurlkill 11.008
EMBOSS 11.418
Sillero 11.008
Patrickios 11.506
IPC_peptide 11.184
IPC2_peptide 8.916
IPC2.peptide.svr19 8.69
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
1
6
2255
74
781
375.8
41.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.987 ± 0.579
1.596 ± 0.718
4.169 ± 0.61
4.745 ± 0.364
3.858 ± 0.54
8.115 ± 0.961
3.193 ± 0.522
3.769 ± 0.56
5.543 ± 0.477
8.603 ± 0.756
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.173 ± 0.726
4.922 ± 0.511
7.361 ± 1.455
4.789 ± 0.76
2.661 ± 0.326
8.204 ± 0.729
7.273 ± 0.337
6.874 ± 0.775
1.863 ± 0.396
4.302 ± 0.707
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here