Xylella phage Salvo
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5Q9T1|V5Q9T1_9CAUD Uncharacterized protein OS=Xylella phage Salvo OX=1415147 GN=Salvo_10 PE=4 SV=1
MM1 pKa = 7.79 CEE3 pKa = 4.21 LDD5 pKa = 3.34 VTAIVASIAPRR16 pKa = 11.84 DD17 pKa = 3.74 YY18 pKa = 10.55 SASVAEE24 pKa = 4.76 LGNDD28 pKa = 3.14 AGRR31 pKa = 11.84 ITWEE35 pKa = 4.19 AACEE39 pKa = 3.8 DD40 pKa = 3.35 ARR42 pKa = 11.84 EE43 pKa = 4.0 LFGEE47 pKa = 4.28 HH48 pKa = 6.62 FDD50 pKa = 3.49 RR51 pKa = 11.84 AAFDD55 pKa = 3.96 EE56 pKa = 4.6 YY57 pKa = 10.88 FSHH60 pKa = 7.22 FGAWDD65 pKa = 3.62 AEE67 pKa = 3.94 EE68 pKa = 4.7 LAAHH72 pKa = 6.24 TDD74 pKa = 3.79 EE75 pKa = 4.59 EE76 pKa = 4.93 CAALMLQFIAGDD88 pKa = 3.4 MRR90 pKa = 11.84 EE91 pKa = 4.34 ADD93 pKa = 3.72 FSSYY97 pKa = 11.61 ADD99 pKa = 3.27 IEE101 pKa = 4.6 GGAEE105 pKa = 3.97 PFTDD109 pKa = 3.74 EE110 pKa = 3.83 WWPQYY115 pKa = 8.13 EE116 pKa = 4.35 TASEE120 pKa = 4.19 AGTVAGRR127 pKa = 11.84 FFRR130 pKa = 11.84 ADD132 pKa = 3.54 DD133 pKa = 3.7 GRR135 pKa = 11.84 VFYY138 pKa = 10.84 YY139 pKa = 10.11 IGEE142 pKa = 4.14
Molecular weight: 15.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.91
IPC_protein 3.872
Toseland 3.681
ProMoST 3.986
Dawson 3.846
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.126
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.986
Patrickios 1.875
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|V5Q9T6|V5Q9T6_9CAUD Uncharacterized protein OS=Xylella phage Salvo OX=1415147 GN=Salvo_15 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 9.41 GTAQRR7 pKa = 11.84 AARR10 pKa = 11.84 AALTRR15 pKa = 11.84 PRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 HH20 pKa = 5.04 AGARR24 pKa = 11.84 QPRR27 pKa = 11.84 AARR30 pKa = 11.84 VEE32 pKa = 3.87 NRR34 pKa = 11.84 AA35 pKa = 3.35
Molecular weight: 3.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.149
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.15
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
17776
35
1445
246.9
27.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.739 ± 0.69
0.866 ± 0.116
6.683 ± 0.311
6.666 ± 0.264
3.69 ± 0.171
7.516 ± 0.317
1.873 ± 0.217
4.484 ± 0.17
4.967 ± 0.358
8.101 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.622 ± 0.153
3.837 ± 0.232
4.489 ± 0.27
3.707 ± 0.302
7.139 ± 0.282
5.575 ± 0.201
5.513 ± 0.279
6.616 ± 0.264
1.8 ± 0.175
3.117 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here