Wenzhouxiangella marina
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3023 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K0XUA3|A0A0K0XUA3_9GAMM Uncharacterized protein OS=Wenzhouxiangella marina OX=1579979 GN=WM2015_913 PE=4 SV=1
MM1 pKa = 7.68 AAGLLATHH9 pKa = 7.65 AIATVEE15 pKa = 4.02 YY16 pKa = 9.61 DD17 pKa = 3.35 QDD19 pKa = 3.78 VTPTIIFGAGNNNGAFTTDD38 pKa = 2.7 RR39 pKa = 11.84 FNGVEE44 pKa = 3.35 IGLRR48 pKa = 11.84 AKK50 pKa = 10.39 LRR52 pKa = 11.84 YY53 pKa = 9.55 DD54 pKa = 3.64 EE55 pKa = 5.61 NGVPQNVFNSNGDD68 pKa = 3.22 GSYY71 pKa = 9.42 TFYY74 pKa = 10.85 TRR76 pKa = 11.84 SRR78 pKa = 11.84 PNFPDD83 pKa = 3.58 GQRR86 pKa = 11.84 GEE88 pKa = 4.52 WSFEE92 pKa = 3.7 WAVNTDD98 pKa = 3.9 FDD100 pKa = 5.05 GSSLLNLDD108 pKa = 4.04 DD109 pKa = 5.14 LSYY112 pKa = 11.3 EE113 pKa = 4.4 LGLDD117 pKa = 3.7 ADD119 pKa = 4.99 PGPGTNFLVFDD130 pKa = 6.08 PITPSAEE137 pKa = 3.99 APCWDD142 pKa = 3.89 HH143 pKa = 7.57 AIGNNGTGNGGGTAVNCITDD163 pKa = 4.0 PNPGQSYY170 pKa = 11.16 AGLLTDD176 pKa = 5.04 NNVAQNSWQYY186 pKa = 11.48 GFFLIDD192 pKa = 3.38 EE193 pKa = 4.72 LADD196 pKa = 3.59 YY197 pKa = 10.7 DD198 pKa = 3.67 PRR200 pKa = 11.84 LPGRR204 pKa = 11.84 YY205 pKa = 7.58 TIYY208 pKa = 10.58 LQASNGSGVVARR220 pKa = 11.84 SEE222 pKa = 4.0 IEE224 pKa = 4.36 VIVVDD229 pKa = 4.49 PPLAFDD235 pKa = 3.92 QNVTPEE241 pKa = 4.66 AIFGSGNANGEE252 pKa = 4.1 FTTDD256 pKa = 2.85 RR257 pKa = 11.84 RR258 pKa = 11.84 NDD260 pKa = 3.05 IEE262 pKa = 4.86 LGLRR266 pKa = 11.84 GKK268 pKa = 10.19 LRR270 pKa = 11.84 YY271 pKa = 8.88 NASGVPEE278 pKa = 3.9 NTFNSNGDD286 pKa = 3.36 GTYY289 pKa = 10.7 SFAPRR294 pKa = 11.84 LVPGGSPLRR303 pKa = 11.84 AEE305 pKa = 4.68 WGFEE309 pKa = 3.43 WSVNTNYY316 pKa = 10.23 QGGAGPNVDD325 pKa = 3.27 AYY327 pKa = 9.76 TYY329 pKa = 10.93 EE330 pKa = 4.91 LGLDD334 pKa = 3.89 ADD336 pKa = 4.56 PSLGTDD342 pKa = 4.07 FLVFDD347 pKa = 6.28 PITPTAEE354 pKa = 4.22 VPCWDD359 pKa = 3.72 HH360 pKa = 8.34 AMGNNGTPNGGGTSANCLGDD380 pKa = 3.84 PNAGATYY387 pKa = 10.81 QNLIANNNVAQNSWRR402 pKa = 11.84 YY403 pKa = 9.05 EE404 pKa = 3.94 FFASGPIAGFDD415 pKa = 3.53 PTVDD419 pKa = 3.11 GSYY422 pKa = 10.43 RR423 pKa = 11.84 IYY425 pKa = 10.67 LAAYY429 pKa = 8.13 NADD432 pKa = 3.33 GVEE435 pKa = 4.37 VARR438 pKa = 11.84 SDD440 pKa = 3.03 IDD442 pKa = 3.45 ILVGSASGGTVSDD455 pKa = 4.52 VEE457 pKa = 4.82 LSMSTTATGTQFTGDD472 pKa = 4.01 AITYY476 pKa = 9.22 QLVASNTDD484 pKa = 3.28 LAKK487 pKa = 10.47 AANVSILNVLPDD499 pKa = 3.7 NLSFVAGSCDD509 pKa = 3.65 DD510 pKa = 3.82 GSVANVAGQSVDD522 pKa = 3.72 FALADD527 pKa = 4.03 LPSGASTICTIDD539 pKa = 3.39 TVVASSGTIVNSASVAADD557 pKa = 3.26 NDD559 pKa = 3.3 GDD561 pKa = 4.36 ANNNAASVRR570 pKa = 11.84 LLGVIEE576 pKa = 4.45 SVALTGDD583 pKa = 3.31 IPSPTDD589 pKa = 3.16 NDD591 pKa = 3.36 YY592 pKa = 10.87 TRR594 pKa = 11.84 INDD597 pKa = 3.47 VVQIAGPGDD606 pKa = 3.66 QIVLNGVFDD615 pKa = 4.3 WNEE618 pKa = 3.98 SNAFASWALGSDD630 pKa = 4.55 GIDD633 pKa = 3.22 GTVDD637 pKa = 2.63 DD638 pKa = 4.12 WTIYY642 pKa = 10.74 VPDD645 pKa = 4.32 GLADD649 pKa = 3.68 LTITATAPGDD659 pKa = 3.75 ATIKK663 pKa = 10.95 GPGDD667 pKa = 3.35 LAGVDD672 pKa = 3.89 LEE674 pKa = 4.89 GFLLVYY680 pKa = 8.25 GTNPGLEE687 pKa = 3.79 ISNLVIEE694 pKa = 5.26 DD695 pKa = 3.49 FDD697 pKa = 4.06 VAIGIYY703 pKa = 10.43 YY704 pKa = 10.39 NGGGVNVYY712 pKa = 11.16 DD713 pKa = 4.19 NLTIIDD719 pKa = 4.35 NFIAMPRR726 pKa = 11.84 DD727 pKa = 3.48 VAGNSQGGEE736 pKa = 3.71 AFQNIGIHH744 pKa = 6.14 YY745 pKa = 10.13 SFGDD749 pKa = 3.56 NILIARR755 pKa = 11.84 NVIEE759 pKa = 4.57 IPGDD763 pKa = 3.82 SASTASLRR771 pKa = 11.84 AAQVAMQSNTSGGAYY786 pKa = 9.07 EE787 pKa = 4.3 GLVIEE792 pKa = 5.02 DD793 pKa = 3.44 NEE795 pKa = 4.08 IRR797 pKa = 11.84 ILLAQAEE804 pKa = 4.53 IPAGIIGIWEE814 pKa = 4.2 NGNAHH819 pKa = 6.39 TSNITVRR826 pKa = 11.84 NNRR829 pKa = 11.84 FINLDD834 pKa = 3.61 PANDD838 pKa = 3.81 PSLNDD843 pKa = 3.17 QEE845 pKa = 4.69 AFRR848 pKa = 11.84 ITSHH852 pKa = 6.45 SSASSTTRR860 pKa = 11.84 YY861 pKa = 8.54 EE862 pKa = 3.8 GNYY865 pKa = 10.47 AEE867 pKa = 4.81 GANVGYY873 pKa = 10.36 GWLSFDD879 pKa = 3.75 TYY881 pKa = 11.43 GADD884 pKa = 3.51 FSTRR888 pKa = 11.84 DD889 pKa = 3.62 PIEE892 pKa = 4.25 FVSNTAVDD900 pKa = 3.85 NLTGILIDD908 pKa = 3.95 SNGAADD914 pKa = 4.32 LSCNRR919 pKa = 11.84 IHH921 pKa = 7.49 GNDD924 pKa = 3.87 LGLSNITQAGRR935 pKa = 11.84 ISLADD940 pKa = 4.22 DD941 pKa = 3.37 NWWGCNAGPNAGDD954 pKa = 3.67 CDD956 pKa = 4.34 AYY958 pKa = 10.57 DD959 pKa = 3.57 TGLTTDD965 pKa = 2.91 RR966 pKa = 11.84 WLVAGLTADD975 pKa = 3.78 AGTVLINSTTGLNLDD990 pKa = 4.25 LRR992 pKa = 11.84 SNSDD996 pKa = 3.21 GNEE999 pKa = 4.04 VTSCTLPATPVILAANEE1016 pKa = 4.34 GSVTPAVGATSAALLDD1032 pKa = 4.1 ADD1034 pKa = 3.85 YY1035 pKa = 8.08 TAPGFATGDD1044 pKa = 3.79 TVTVTVDD1051 pKa = 3.47 AEE1053 pKa = 4.31 VLTVDD1058 pKa = 4.44 FTVEE1062 pKa = 4.18 LPVDD1066 pKa = 4.06 SIFNDD1071 pKa = 3.35 RR1072 pKa = 11.84 FEE1074 pKa = 4.09 NN1075 pKa = 3.72
Molecular weight: 112.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.63
IPC_protein 3.694
Toseland 3.452
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.075
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.808
Patrickios 0.884
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.727
Protein with the highest isoelectric point:
>tr|A0A0K0XRS8|A0A0K0XRS8_9GAMM Glycine--tRNA ligase alpha subunit OS=Wenzhouxiangella marina OX=1579979 GN=glyQ PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 IKK11 pKa = 10.39 RR12 pKa = 11.84 VRR14 pKa = 11.84 THH16 pKa = 5.87 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATASGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.81 RR41 pKa = 11.84 LAATNNRR48 pKa = 3.77
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3023
0
3023
1100236
29
5034
364.0
39.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.293 ± 0.061
0.888 ± 0.016
6.264 ± 0.039
6.606 ± 0.047
3.598 ± 0.028
8.554 ± 0.051
2.072 ± 0.026
4.746 ± 0.028
1.887 ± 0.032
11.339 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.107 ± 0.023
2.638 ± 0.041
5.217 ± 0.032
3.765 ± 0.025
7.827 ± 0.067
5.977 ± 0.047
4.588 ± 0.053
6.926 ± 0.043
1.573 ± 0.021
2.135 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here