Butyrivibrio proteoclasticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5YUR9|A0A1I5YUR9_9FIRM Uncharacterized protein OS=Butyrivibrio proteoclasticus OX=43305 GN=SAMN04487928_1582 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.14KK3 pKa = 9.74NKK5 pKa = 9.95KK6 pKa = 9.39IILIITILVALILLGGCATWYY27 pKa = 10.18VLNNNASDD35 pKa = 3.54NGKK38 pKa = 9.95NNISSVEE45 pKa = 4.01QTNDD49 pKa = 3.05MSPEE53 pKa = 3.76EE54 pKa = 4.4LFQAFCDD61 pKa = 4.04GKK63 pKa = 8.77ITAEE67 pKa = 3.72ARR69 pKa = 11.84SYY71 pKa = 11.73YY72 pKa = 10.71DD73 pKa = 3.23DD74 pKa = 4.05TTWTMDD80 pKa = 3.01NTSFQFEE87 pKa = 4.17EE88 pKa = 4.22ATPEE92 pKa = 4.11EE93 pKa = 4.56EE94 pKa = 4.29FDD96 pKa = 3.9ASLAVTKK103 pKa = 10.43VDD105 pKa = 5.55PVDD108 pKa = 4.36LDD110 pKa = 3.44NDD112 pKa = 3.87GDD114 pKa = 4.08VEE116 pKa = 4.98FILEE120 pKa = 3.84NAMYY124 pKa = 11.16GDD126 pKa = 3.77MCFDD130 pKa = 3.95CKK132 pKa = 10.59DD133 pKa = 3.45GKK135 pKa = 9.53VVCFAQGEE143 pKa = 4.58GTAAMCSFTQYY154 pKa = 11.18DD155 pKa = 3.5GAYY158 pKa = 8.58WIVHH162 pKa = 6.32SDD164 pKa = 3.26TTHH167 pKa = 6.82GGRR170 pKa = 11.84CTYY173 pKa = 11.08VLTKK177 pKa = 10.4FNGNLEE183 pKa = 4.36VVDD186 pKa = 3.87SFKK189 pKa = 11.15FGWEE193 pKa = 3.74DD194 pKa = 3.44WEE196 pKa = 5.41DD197 pKa = 3.86DD198 pKa = 4.53GIKK201 pKa = 9.59TYY203 pKa = 11.18YY204 pKa = 10.73LDD206 pKa = 4.54DD207 pKa = 4.37NEE209 pKa = 4.34ITEE212 pKa = 4.81ADD214 pKa = 3.96YY215 pKa = 11.6KK216 pKa = 11.21DD217 pKa = 5.31LYY219 pKa = 11.48DD220 pKa = 4.47KK221 pKa = 11.42VFF223 pKa = 3.4

Molecular weight: 25.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5U1U5|A0A1I5U1U5_9FIRM Purine-binding chemotaxis protein CheW OS=Butyrivibrio proteoclasticus OX=43305 GN=SAMN04487928_1114 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3761

0

3761

1231681

24

5242

327.5

36.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.089 ± 0.04

1.385 ± 0.017

6.424 ± 0.032

7.28 ± 0.04

4.451 ± 0.033

6.738 ± 0.037

1.606 ± 0.016

7.892 ± 0.036

7.213 ± 0.038

8.479 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.036 ± 0.026

4.903 ± 0.028

3.044 ± 0.021

2.646 ± 0.02

3.905 ± 0.027

6.367 ± 0.04

5.293 ± 0.036

6.816 ± 0.037

0.931 ± 0.014

4.504 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski