Eragrostis minor streak virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6M064|F6M064_9GEMI Replication-associated protein OS=Eragrostis minor streak virus OX=1030595 PE=3 SV=1
MM1 pKa = 7.3SQDD4 pKa = 3.14QDD6 pKa = 3.97TLGSSSDD13 pKa = 3.09GSRR16 pKa = 11.84FRR18 pKa = 11.84ISSKK22 pKa = 9.77QLFLTYY28 pKa = 9.42PRR30 pKa = 11.84CDD32 pKa = 4.15LSPKK36 pKa = 10.61DD37 pKa = 4.34LGLEE41 pKa = 3.98LLQLLIQNKK50 pKa = 8.73PKK52 pKa = 10.32YY53 pKa = 9.66IHH55 pKa = 5.73VTQEE59 pKa = 3.49LHH61 pKa = 7.14KK62 pKa = 11.11DD63 pKa = 4.03GFPHH67 pKa = 7.08LHH69 pKa = 6.84ALVQLEE75 pKa = 4.42KK76 pKa = 11.09KK77 pKa = 10.54LFTRR81 pKa = 11.84RR82 pKa = 11.84QTFFDD87 pKa = 3.65QFLHH91 pKa = 5.63GTKK94 pKa = 10.2FHH96 pKa = 7.18PNIQPARR103 pKa = 11.84DD104 pKa = 3.35ASKK107 pKa = 10.5VLGYY111 pKa = 8.45ITKK114 pKa = 10.44QNGEE118 pKa = 4.07EE119 pKa = 4.33YY120 pKa = 10.34IFGKK124 pKa = 8.31PTLPKK129 pKa = 10.06KK130 pKa = 10.63KK131 pKa = 8.99KK132 pKa = 7.45TAQEE136 pKa = 3.99GRR138 pKa = 11.84DD139 pKa = 3.28QRR141 pKa = 11.84MRR143 pKa = 11.84AIIEE147 pKa = 4.22SSTSKK152 pKa = 10.62QEE154 pKa = 3.88YY155 pKa = 9.13LSMVRR160 pKa = 11.84KK161 pKa = 9.06EE162 pKa = 5.3FPFDD166 pKa = 2.71WATRR170 pKa = 11.84LMQFEE175 pKa = 4.65YY176 pKa = 10.38SASSLFPEE184 pKa = 4.9PPVEE188 pKa = 4.18YY189 pKa = 9.74TSPFPVDD196 pKa = 3.54QLLCPEE202 pKa = 5.95DD203 pKa = 3.01ITEE206 pKa = 4.98IINSEE211 pKa = 4.0WFQVTPYY218 pKa = 10.26SYY220 pKa = 10.15TLIHH224 pKa = 7.22PGTSISQAADD234 pKa = 3.8DD235 pKa = 4.41LQQMQDD241 pKa = 2.19ISMEE245 pKa = 4.08HH246 pKa = 5.87QEE248 pKa = 4.3AGHH251 pKa = 5.64VASTSVDD258 pKa = 3.43QPVLEE263 pKa = 4.25RR264 pKa = 11.84RR265 pKa = 11.84LGLEE269 pKa = 3.8AA270 pKa = 5.82

Molecular weight: 31.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6M063|F6M063_9GEMI Replication-associated protein OS=Eragrostis minor streak virus OX=1030595 PE=3 SV=1
MM1 pKa = 7.97VYY3 pKa = 9.89EE4 pKa = 5.22RR5 pKa = 11.84KK6 pKa = 10.17RR7 pKa = 11.84KK8 pKa = 9.63DD9 pKa = 3.11PRR11 pKa = 11.84RR12 pKa = 11.84SDD14 pKa = 3.35EE15 pKa = 4.2SAGSARR21 pKa = 11.84KK22 pKa = 9.44RR23 pKa = 11.84PRR25 pKa = 11.84APAGRR30 pKa = 11.84SMVSAVRR37 pKa = 11.84RR38 pKa = 11.84PALQIRR44 pKa = 11.84EE45 pKa = 4.45YY46 pKa = 9.92PWQTLTQLPIKK57 pKa = 9.36ITDD60 pKa = 3.63GVIFMVNTIDD70 pKa = 4.92PGTGDD75 pKa = 3.78DD76 pKa = 4.61QRR78 pKa = 11.84SKK80 pKa = 10.43HH81 pKa = 4.41QTMLYY86 pKa = 10.32KK87 pKa = 10.1MSFNCVLWPDD97 pKa = 3.54EE98 pKa = 4.2TTALIVAPFRR108 pKa = 11.84VNFWLVYY115 pKa = 10.26DD116 pKa = 4.84AAPQGKK122 pKa = 9.2LPALSDD128 pKa = 3.55IFSVPYY134 pKa = 9.33TKK136 pKa = 9.78WGNTWNVSRR145 pKa = 11.84TNVHH149 pKa = 5.71RR150 pKa = 11.84FVVKK154 pKa = 10.39RR155 pKa = 11.84KK156 pKa = 6.82WHH158 pKa = 5.36VDD160 pKa = 3.4YY161 pKa = 11.08NSSGTAVGKK170 pKa = 9.85KK171 pKa = 8.14QSPGSEE177 pKa = 4.04DD178 pKa = 3.92FPVKK182 pKa = 10.68NVVEE186 pKa = 4.28CNKK189 pKa = 9.57FFEE192 pKa = 4.32KK193 pKa = 10.73LRR195 pKa = 11.84VKK197 pKa = 9.34TEE199 pKa = 3.76WLNTTDD205 pKa = 3.5GTIGSVKK212 pKa = 10.21KK213 pKa = 10.32GALYY217 pKa = 10.53LVANTRR223 pKa = 11.84QMPAGDD229 pKa = 4.51AVTTTCTTYY238 pKa = 9.54MQGSTRR244 pKa = 11.84LYY246 pKa = 9.94FKK248 pKa = 11.0SLGYY252 pKa = 9.73QQ253 pKa = 3.03

Molecular weight: 28.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

974

99

352

243.5

27.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.057 ± 1.064

1.129 ± 0.156

5.339 ± 0.509

5.441 ± 0.725

4.928 ± 0.295

5.749 ± 0.408

2.567 ± 0.55

4.723 ± 0.474

6.674 ± 1.006

8.932 ± 1.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.053 ± 0.256

2.567 ± 0.585

6.982 ± 0.575

6.057 ± 0.877

6.057 ± 0.855

8.008 ± 0.97

6.263 ± 0.955

5.031 ± 1.177

1.54 ± 0.365

3.901 ± 0.73
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski