Burkholderiales bacterium PBB2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257FIT8|A0A257FIT8_9BURK Haemagg_act domain-containing protein OS=Burkholderiales bacterium PBB2 OX=2015564 GN=CFE41_18500 PE=4 SV=1
MM1 pKa = 7.35NLFASTRR8 pKa = 11.84LAAPALACAALLCSALPAQADD29 pKa = 3.5MSFYY33 pKa = 10.86SSQAAFNAAISAPVTDD49 pKa = 4.44SFNDD53 pKa = 3.58LFWAQGPNALYY64 pKa = 10.31RR65 pKa = 11.84EE66 pKa = 4.31PGGGYY71 pKa = 9.76SYY73 pKa = 11.49VVNALKK79 pKa = 10.47FDD81 pKa = 3.77GSDD84 pKa = 3.24YY85 pKa = 11.15SSFYY89 pKa = 10.87NAGSDD94 pKa = 3.63DD95 pKa = 4.58DD96 pKa = 4.37VWLSTDD102 pKa = 2.87QATDD106 pKa = 3.6VISFDD111 pKa = 3.43FTGGDD116 pKa = 2.94KK117 pKa = 11.0VYY119 pKa = 10.76GVGGFFFGSDD129 pKa = 3.32MLGAFQPGQSIKK141 pKa = 11.02LNALDD146 pKa = 4.11ADD148 pKa = 3.87GVEE151 pKa = 3.76QVVIIEE157 pKa = 4.1NAQPGSFYY165 pKa = 11.31GFASTSLIRR174 pKa = 11.84YY175 pKa = 8.21FEE177 pKa = 4.08ISAVQPADD185 pKa = 2.99GLTWVAANDD194 pKa = 3.88LVLGTVAAVPEE205 pKa = 4.41PSSSALLLGGLGLLAWGATRR225 pKa = 11.84RR226 pKa = 11.84SAEE229 pKa = 4.29NKK231 pKa = 10.42DD232 pKa = 3.12MATPKK237 pKa = 10.76GG238 pKa = 3.61

Molecular weight: 24.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257FII7|A0A257FII7_9BURK DNA starvation/stationary phase protection protein OS=Burkholderiales bacterium PBB2 OX=2015564 GN=CFE41_18575 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.73QASKK9 pKa = 7.81TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4553

0

4553

1629190

29

3843

357.8

38.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.254 ± 0.046

0.902 ± 0.011

4.855 ± 0.022

5.477 ± 0.036

3.309 ± 0.022

8.376 ± 0.05

2.158 ± 0.017

3.734 ± 0.026

2.993 ± 0.032

11.933 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.018

2.523 ± 0.026

5.346 ± 0.033

4.841 ± 0.029

7.092 ± 0.038

6.195 ± 0.041

4.445 ± 0.033

6.746 ± 0.031

1.584 ± 0.018

2.038 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski