Amino acid dipepetide frequency for Erwinia phage vB_EamM_Joad

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.082AlaAla: 6.082 ± 0.435
0.591AlaCys: 0.591 ± 0.091
4.471AlaAsp: 4.471 ± 0.266
4.833AlaGlu: 4.833 ± 0.289
2.739AlaPhe: 2.739 ± 0.168
4.658AlaGly: 4.658 ± 0.276
1.155AlaHis: 1.155 ± 0.131
4.014AlaIle: 4.014 ± 0.266
4.309AlaLys: 4.309 ± 0.35
6.565AlaLeu: 6.565 ± 0.244
2.148AlaMet: 2.148 ± 0.193
4.041AlaAsn: 4.041 ± 0.244
2.672AlaPro: 2.672 ± 0.267
2.86AlaGln: 2.86 ± 0.218
3.141AlaArg: 3.141 ± 0.218
3.343AlaSer: 3.343 ± 0.286
4.108AlaThr: 4.108 ± 0.239
4.82AlaVal: 4.82 ± 0.258
0.524AlaTrp: 0.524 ± 0.091
2.363AlaTyr: 2.363 ± 0.181
0.0AlaXaa: 0.0 ± 0.0
Cys
0.564CysAla: 0.564 ± 0.087
0.081CysCys: 0.081 ± 0.034
0.564CysAsp: 0.564 ± 0.088
0.537CysGlu: 0.537 ± 0.086
0.242CysPhe: 0.242 ± 0.05
0.47CysGly: 0.47 ± 0.079
0.201CysHis: 0.201 ± 0.052
0.644CysIle: 0.644 ± 0.099
0.604CysLys: 0.604 ± 0.085
0.846CysLeu: 0.846 ± 0.121
0.322CysMet: 0.322 ± 0.062
0.403CysAsn: 0.403 ± 0.081
0.416CysPro: 0.416 ± 0.078
0.295CysGln: 0.295 ± 0.065
0.497CysArg: 0.497 ± 0.086
0.577CysSer: 0.577 ± 0.081
0.577CysThr: 0.577 ± 0.088
0.698CysVal: 0.698 ± 0.093
0.107CysTrp: 0.107 ± 0.042
0.456CysTyr: 0.456 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
4.189AspAla: 4.189 ± 0.28
0.55AspCys: 0.55 ± 0.093
3.88AspAsp: 3.88 ± 0.255
3.732AspGlu: 3.732 ± 0.231
3.141AspPhe: 3.141 ± 0.186
4.001AspGly: 4.001 ± 0.272
1.047AspHis: 1.047 ± 0.128
4.148AspIle: 4.148 ± 0.244
4.229AspLys: 4.229 ± 0.319
5.665AspLeu: 5.665 ± 0.248
1.853AspMet: 1.853 ± 0.168
3.625AspAsn: 3.625 ± 0.243
2.685AspPro: 2.685 ± 0.201
1.826AspGln: 1.826 ± 0.167
2.86AspArg: 2.86 ± 0.191
3.303AspSer: 3.303 ± 0.172
3.759AspThr: 3.759 ± 0.22
4.739AspVal: 4.739 ± 0.28
1.061AspTrp: 1.061 ± 0.116
2.645AspTyr: 2.645 ± 0.186
0.0AspXaa: 0.0 ± 0.0
Glu
4.403GluAla: 4.403 ± 0.264
0.631GluCys: 0.631 ± 0.102
3.356GluAsp: 3.356 ± 0.195
4.39GluGlu: 4.39 ± 0.36
2.94GluPhe: 2.94 ± 0.226
3.759GluGly: 3.759 ± 0.264
1.423GluHis: 1.423 ± 0.155
3.907GluIle: 3.907 ± 0.201
3.544GluLys: 3.544 ± 0.253
6.753GluLeu: 6.753 ± 0.317
1.853GluMet: 1.853 ± 0.16
3.182GluAsn: 3.182 ± 0.202
1.705GluPro: 1.705 ± 0.178
2.752GluGln: 2.752 ± 0.205
3.101GluArg: 3.101 ± 0.217
3.074GluSer: 3.074 ± 0.214
3.826GluThr: 3.826 ± 0.217
4.497GluVal: 4.497 ± 0.315
0.618GluTrp: 0.618 ± 0.079
2.336GluTyr: 2.336 ± 0.217
0.0GluXaa: 0.0 ± 0.0
Phe
2.497PheAla: 2.497 ± 0.205
0.443PheCys: 0.443 ± 0.085
2.913PheAsp: 2.913 ± 0.178
2.349PheGlu: 2.349 ± 0.195
1.786PhePhe: 1.786 ± 0.173
2.712PheGly: 2.712 ± 0.178
1.141PheHis: 1.141 ± 0.115
2.564PheIle: 2.564 ± 0.185
2.886PheLys: 2.886 ± 0.21
3.316PheLeu: 3.316 ± 0.213
1.504PheMet: 1.504 ± 0.126
3.316PheAsn: 3.316 ± 0.236
1.356PhePro: 1.356 ± 0.128
1.155PheGln: 1.155 ± 0.116
2.161PheArg: 2.161 ± 0.162
3.034PheSer: 3.034 ± 0.194
2.927PheThr: 2.927 ± 0.199
2.658PheVal: 2.658 ± 0.235
0.43PheTrp: 0.43 ± 0.081
1.839PheTyr: 1.839 ± 0.145
0.0PheXaa: 0.0 ± 0.0
Gly
3.732GlyAla: 3.732 ± 0.271
0.564GlyCys: 0.564 ± 0.08
3.987GlyAsp: 3.987 ± 0.25
3.893GlyGlu: 3.893 ± 0.243
2.551GlyPhe: 2.551 ± 0.179
4.524GlyGly: 4.524 ± 0.4
0.953GlyHis: 0.953 ± 0.1
4.041GlyIle: 4.041 ± 0.199
4.162GlyLys: 4.162 ± 0.23
5.41GlyLeu: 5.41 ± 0.307
2.027GlyMet: 2.027 ± 0.227
4.215GlyAsn: 4.215 ± 0.278
1.477GlyPro: 1.477 ± 0.191
2.296GlyGln: 2.296 ± 0.199
3.088GlyArg: 3.088 ± 0.231
4.068GlySer: 4.068 ± 0.284
4.162GlyThr: 4.162 ± 0.266
4.914GlyVal: 4.914 ± 0.275
0.94GlyTrp: 0.94 ± 0.097
2.927GlyTyr: 2.927 ± 0.188
0.0GlyXaa: 0.0 ± 0.0
His
1.423HisAla: 1.423 ± 0.164
0.255HisCys: 0.255 ± 0.056
0.967HisAsp: 0.967 ± 0.127
0.899HisGlu: 0.899 ± 0.119
1.007HisPhe: 1.007 ± 0.114
1.101HisGly: 1.101 ± 0.124
0.524HisHis: 0.524 ± 0.092
1.181HisIle: 1.181 ± 0.109
1.101HisLys: 1.101 ± 0.135
1.987HisLeu: 1.987 ± 0.161
0.618HisMet: 0.618 ± 0.096
1.02HisAsn: 1.02 ± 0.114
0.993HisPro: 0.993 ± 0.121
1.02HisGln: 1.02 ± 0.108
0.98HisArg: 0.98 ± 0.124
1.235HisSer: 1.235 ± 0.129
0.993HisThr: 0.993 ± 0.123
1.208HisVal: 1.208 ± 0.127
0.201HisTrp: 0.201 ± 0.044
0.953HisTyr: 0.953 ± 0.117
0.0HisXaa: 0.0 ± 0.0
Ile
4.444IleAla: 4.444 ± 0.281
0.644IleCys: 0.644 ± 0.1
3.96IleAsp: 3.96 ± 0.262
3.92IleGlu: 3.92 ± 0.233
2.054IlePhe: 2.054 ± 0.167
3.262IleGly: 3.262 ± 0.234
1.262IleHis: 1.262 ± 0.131
3.182IleIle: 3.182 ± 0.214
4.591IleLys: 4.591 ± 0.278
4.054IleLeu: 4.054 ± 0.231
1.544IleMet: 1.544 ± 0.173
4.229IleAsn: 4.229 ± 0.291
3.141IlePro: 3.141 ± 0.253
2.336IleGln: 2.336 ± 0.149
3.182IleArg: 3.182 ± 0.206
3.786IleSer: 3.786 ± 0.245
4.309IleThr: 4.309 ± 0.223
3.37IleVal: 3.37 ± 0.214
0.631IleTrp: 0.631 ± 0.09
2.094IleTyr: 2.094 ± 0.185
0.0IleXaa: 0.0 ± 0.0
Lys
4.82LysAla: 4.82 ± 0.297
0.376LysCys: 0.376 ± 0.065
4.403LysAsp: 4.403 ± 0.264
4.578LysGlu: 4.578 ± 0.309
2.309LysPhe: 2.309 ± 0.2
3.665LysGly: 3.665 ± 0.271
1.463LysHis: 1.463 ± 0.172
3.383LysIle: 3.383 ± 0.208
3.678LysLys: 3.678 ± 0.275
6.028LysLeu: 6.028 ± 0.247
1.571LysMet: 1.571 ± 0.146
3.021LysAsn: 3.021 ± 0.187
2.269LysPro: 2.269 ± 0.192
2.658LysGln: 2.658 ± 0.195
2.953LysArg: 2.953 ± 0.22
3.209LysSer: 3.209 ± 0.236
3.665LysThr: 3.665 ± 0.25
4.632LysVal: 4.632 ± 0.231
0.618LysTrp: 0.618 ± 0.087
2.43LysTyr: 2.43 ± 0.17
0.0LysXaa: 0.0 ± 0.0
Leu
6.498LeuAla: 6.498 ± 0.38
0.926LeuCys: 0.926 ± 0.1
5.437LeuAsp: 5.437 ± 0.243
5.437LeuGlu: 5.437 ± 0.29
3.558LeuPhe: 3.558 ± 0.232
5.236LeuGly: 5.236 ± 0.28
1.732LeuHis: 1.732 ± 0.165
5.397LeuIle: 5.397 ± 0.272
5.706LeuLys: 5.706 ± 0.307
7.223LeuLeu: 7.223 ± 0.344
2.672LeuMet: 2.672 ± 0.177
5.236LeuAsn: 5.236 ± 0.283
4.054LeuPro: 4.054 ± 0.243
2.86LeuGln: 2.86 ± 0.187
3.866LeuArg: 3.866 ± 0.243
6.337LeuSer: 6.337 ± 0.282
6.914LeuThr: 6.914 ± 0.355
5.813LeuVal: 5.813 ± 0.314
0.832LeuTrp: 0.832 ± 0.105
3.397LeuTyr: 3.397 ± 0.224
0.0LeuXaa: 0.0 ± 0.0
Met
2.014MetAla: 2.014 ± 0.175
0.282MetCys: 0.282 ± 0.066
1.893MetAsp: 1.893 ± 0.175
1.759MetGlu: 1.759 ± 0.134
1.114MetPhe: 1.114 ± 0.124
1.96MetGly: 1.96 ± 0.147
0.497MetHis: 0.497 ± 0.088
1.571MetIle: 1.571 ± 0.153
2.054MetLys: 2.054 ± 0.143
2.416MetLeu: 2.416 ± 0.194
1.047MetMet: 1.047 ± 0.167
1.678MetAsn: 1.678 ± 0.164
1.02MetPro: 1.02 ± 0.131
1.114MetGln: 1.114 ± 0.119
1.168MetArg: 1.168 ± 0.123
2.578MetSer: 2.578 ± 0.213
2.094MetThr: 2.094 ± 0.173
2.175MetVal: 2.175 ± 0.166
0.295MetTrp: 0.295 ± 0.06
0.846MetTyr: 0.846 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.759AsnAla: 3.759 ± 0.216
0.389AsnCys: 0.389 ± 0.072
3.504AsnAsp: 3.504 ± 0.206
3.303AsnGlu: 3.303 ± 0.173
2.269AsnPhe: 2.269 ± 0.163
4.927AsnGly: 4.927 ± 0.281
1.181AsnHis: 1.181 ± 0.114
3.262AsnIle: 3.262 ± 0.212
4.001AsnLys: 4.001 ± 0.235
4.658AsnLeu: 4.658 ± 0.255
1.369AsnMet: 1.369 ± 0.14
3.504AsnAsn: 3.504 ± 0.293
3.343AsnPro: 3.343 ± 0.254
2.376AsnGln: 2.376 ± 0.192
3.034AsnArg: 3.034 ± 0.172
2.967AsnSer: 2.967 ± 0.213
3.88AsnThr: 3.88 ± 0.204
3.289AsnVal: 3.289 ± 0.176
0.671AsnTrp: 0.671 ± 0.109
2.296AsnTyr: 2.296 ± 0.174
0.0AsnXaa: 0.0 ± 0.0
Pro
3.37ProAla: 3.37 ± 0.219
0.268ProCys: 0.268 ± 0.054
2.672ProAsp: 2.672 ± 0.191
3.047ProGlu: 3.047 ± 0.237
1.866ProPhe: 1.866 ± 0.17
2.43ProGly: 2.43 ± 0.234
0.752ProHis: 0.752 ± 0.104
2.067ProIle: 2.067 ± 0.155
2.242ProLys: 2.242 ± 0.184
3.222ProLeu: 3.222 ± 0.206
1.087ProMet: 1.087 ± 0.118
2.242ProAsn: 2.242 ± 0.19
1.249ProPro: 1.249 ± 0.124
1.396ProGln: 1.396 ± 0.126
1.53ProArg: 1.53 ± 0.17
2.685ProSer: 2.685 ± 0.224
2.631ProThr: 2.631 ± 0.203
3.343ProVal: 3.343 ± 0.261
0.376ProTrp: 0.376 ± 0.08
1.624ProTyr: 1.624 ± 0.155
0.0ProXaa: 0.0 ± 0.0
Gln
2.658GlnAla: 2.658 ± 0.23
0.376GlnCys: 0.376 ± 0.068
2.014GlnAsp: 2.014 ± 0.167
2.269GlnGlu: 2.269 ± 0.17
1.638GlnPhe: 1.638 ± 0.153
2.215GlnGly: 2.215 ± 0.232
0.886GlnHis: 0.886 ± 0.1
2.215GlnIle: 2.215 ± 0.184
1.705GlnLys: 1.705 ± 0.141
4.444GlnLeu: 4.444 ± 0.226
1.342GlnMet: 1.342 ± 0.163
1.557GlnAsn: 1.557 ± 0.145
1.396GlnPro: 1.396 ± 0.147
2.0GlnGln: 2.0 ± 0.195
1.772GlnArg: 1.772 ± 0.145
2.108GlnSer: 2.108 ± 0.183
2.269GlnThr: 2.269 ± 0.187
2.604GlnVal: 2.604 ± 0.195
0.43GlnTrp: 0.43 ± 0.075
1.638GlnTyr: 1.638 ± 0.132
0.0GlnXaa: 0.0 ± 0.0
Arg
2.873ArgAla: 2.873 ± 0.178
0.631ArgCys: 0.631 ± 0.099
2.591ArgAsp: 2.591 ± 0.206
2.913ArgGlu: 2.913 ± 0.189
2.309ArgPhe: 2.309 ± 0.176
2.9ArgGly: 2.9 ± 0.183
1.128ArgHis: 1.128 ± 0.119
3.021ArgIle: 3.021 ± 0.217
3.034ArgLys: 3.034 ± 0.217
4.685ArgLeu: 4.685 ± 0.244
1.624ArgMet: 1.624 ± 0.146
2.927ArgAsn: 2.927 ± 0.215
1.732ArgPro: 1.732 ± 0.165
1.866ArgGln: 1.866 ± 0.162
2.363ArgArg: 2.363 ± 0.226
2.698ArgSer: 2.698 ± 0.187
2.94ArgThr: 2.94 ± 0.178
2.953ArgVal: 2.953 ± 0.21
0.631ArgTrp: 0.631 ± 0.094
2.202ArgTyr: 2.202 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
4.121SerAla: 4.121 ± 0.289
0.336SerCys: 0.336 ± 0.068
3.625SerAsp: 3.625 ± 0.179
3.397SerGlu: 3.397 ± 0.224
3.101SerPhe: 3.101 ± 0.226
3.987SerGly: 3.987 ± 0.262
0.913SerHis: 0.913 ± 0.103
3.88SerIle: 3.88 ± 0.276
3.477SerLys: 3.477 ± 0.252
5.33SerLeu: 5.33 ± 0.227
1.705SerMet: 1.705 ± 0.15
3.316SerAsn: 3.316 ± 0.23
2.403SerPro: 2.403 ± 0.172
2.215SerGln: 2.215 ± 0.188
2.86SerArg: 2.86 ± 0.2
3.786SerSer: 3.786 ± 0.294
3.705SerThr: 3.705 ± 0.233
4.484SerVal: 4.484 ± 0.217
0.819SerTrp: 0.819 ± 0.1
2.43SerTyr: 2.43 ± 0.176
0.0SerXaa: 0.0 ± 0.0
Thr
4.712ThrAla: 4.712 ± 0.28
0.416ThrCys: 0.416 ± 0.081
4.148ThrAsp: 4.148 ± 0.231
3.746ThrGlu: 3.746 ± 0.217
3.034ThrPhe: 3.034 ± 0.238
4.954ThrGly: 4.954 ± 0.285
1.114ThrHis: 1.114 ± 0.133
3.826ThrIle: 3.826 ± 0.256
3.209ThrLys: 3.209 ± 0.187
5.988ThrLeu: 5.988 ± 0.268
1.718ThrMet: 1.718 ± 0.148
3.276ThrAsn: 3.276 ± 0.193
3.141ThrPro: 3.141 ± 0.22
2.43ThrGln: 2.43 ± 0.158
2.94ThrArg: 2.94 ± 0.196
3.746ThrSer: 3.746 ± 0.226
4.256ThrThr: 4.256 ± 0.291
5.182ThrVal: 5.182 ± 0.279
0.712ThrTrp: 0.712 ± 0.1
2.497ThrTyr: 2.497 ± 0.18
0.0ThrXaa: 0.0 ± 0.0
Val
4.524ValAla: 4.524 ± 0.317
0.685ValCys: 0.685 ± 0.099
5.115ValAsp: 5.115 ± 0.29
4.283ValGlu: 4.283 ± 0.247
2.927ValPhe: 2.927 ± 0.23
3.866ValGly: 3.866 ± 0.26
1.128ValHis: 1.128 ± 0.099
4.712ValIle: 4.712 ± 0.272
4.618ValLys: 4.618 ± 0.24
5.853ValLeu: 5.853 ± 0.339
2.161ValMet: 2.161 ± 0.162
3.866ValAsn: 3.866 ± 0.215
3.128ValPro: 3.128 ± 0.214
2.0ValGln: 2.0 ± 0.182
3.182ValArg: 3.182 ± 0.224
4.551ValSer: 4.551 ± 0.309
4.726ValThr: 4.726 ± 0.28
4.981ValVal: 4.981 ± 0.307
0.738ValTrp: 0.738 ± 0.094
2.739ValTyr: 2.739 ± 0.183
0.0ValXaa: 0.0 ± 0.0
Trp
0.497TrpAla: 0.497 ± 0.08
0.175TrpCys: 0.175 ± 0.039
0.899TrpAsp: 0.899 ± 0.098
0.712TrpGlu: 0.712 ± 0.102
0.618TrpPhe: 0.618 ± 0.084
0.631TrpGly: 0.631 ± 0.103
0.175TrpHis: 0.175 ± 0.063
0.725TrpIle: 0.725 ± 0.107
0.698TrpLys: 0.698 ± 0.103
0.98TrpLeu: 0.98 ± 0.103
0.282TrpMet: 0.282 ± 0.056
0.698TrpAsn: 0.698 ± 0.096
0.255TrpPro: 0.255 ± 0.057
0.403TrpGln: 0.403 ± 0.081
0.591TrpArg: 0.591 ± 0.074
0.644TrpSer: 0.644 ± 0.09
0.832TrpThr: 0.832 ± 0.109
0.738TrpVal: 0.738 ± 0.105
0.107TrpTrp: 0.107 ± 0.039
0.524TrpTyr: 0.524 ± 0.079
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.255TyrAla: 2.255 ± 0.178
0.483TyrCys: 0.483 ± 0.075
2.537TyrAsp: 2.537 ± 0.176
2.054TyrGlu: 2.054 ± 0.172
1.786TyrPhe: 1.786 ± 0.143
2.672TyrGly: 2.672 ± 0.179
0.993TyrHis: 0.993 ± 0.124
2.255TyrIle: 2.255 ± 0.168
1.879TyrLys: 1.879 ± 0.146
3.705TyrLeu: 3.705 ± 0.243
1.114TyrMet: 1.114 ± 0.133
2.631TyrAsn: 2.631 ± 0.187
1.665TyrPro: 1.665 ± 0.163
1.678TyrGln: 1.678 ± 0.165
2.752TyrArg: 2.752 ± 0.178
2.161TyrSer: 2.161 ± 0.176
2.484TyrThr: 2.484 ± 0.181
2.685TyrVal: 2.685 ± 0.179
0.443TyrTrp: 0.443 ± 0.068
1.692TyrTyr: 1.692 ± 0.146
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 245 proteins (74489 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski