Gracilibacillus dipsosauri

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli;

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317L148|A0A317L148_9BACI Aminotransferase class V-fold PLP-dependent enzyme OS=Gracilibacillus dipsosauri OX=178340 GN=DLJ74_11320 PE=3 SV=1
MM1 pKa = 7.32SKK3 pKa = 9.7WKK5 pKa = 10.85LMMLVAVTLLTVGLLAACGNAPDD28 pKa = 5.45DD29 pKa = 5.05AGSDD33 pKa = 3.79EE34 pKa = 4.48NNEE37 pKa = 4.0ANASEE42 pKa = 4.17NGEE45 pKa = 4.02GSGDD49 pKa = 3.53EE50 pKa = 4.06GSEE53 pKa = 4.18DD54 pKa = 4.21GEE56 pKa = 4.23VSGPVNIDD64 pKa = 2.9GSSTVLPIMEE74 pKa = 4.51YY75 pKa = 9.38LTYY78 pKa = 10.22EE79 pKa = 4.21YY80 pKa = 10.62NQEE83 pKa = 3.99NPEE86 pKa = 4.03VEE88 pKa = 4.18TALNSSGSGGGFEE101 pKa = 4.38KK102 pKa = 10.19STVGEE107 pKa = 3.98IDD109 pKa = 3.68LSNASRR115 pKa = 11.84PIKK118 pKa = 10.55DD119 pKa = 3.52EE120 pKa = 4.05EE121 pKa = 4.32KK122 pKa = 10.42ATAEE126 pKa = 4.0EE127 pKa = 4.1NGITLHH133 pKa = 6.35EE134 pKa = 4.76IEE136 pKa = 4.92LAYY139 pKa = 10.69DD140 pKa = 3.66GLSVVVSQEE149 pKa = 3.37NDD151 pKa = 3.14FVEE154 pKa = 4.61NLTIDD159 pKa = 3.36QLKK162 pKa = 10.59QIFLADD168 pKa = 3.72GGATKK173 pKa = 10.22WSDD176 pKa = 3.66INPEE180 pKa = 3.84WPEE183 pKa = 3.81EE184 pKa = 4.35DD185 pKa = 2.96IVIYY189 pKa = 10.82SPGHH193 pKa = 6.25DD194 pKa = 4.07SGTFDD199 pKa = 3.78YY200 pKa = 10.64FNEE203 pKa = 4.53VILEE207 pKa = 4.1EE208 pKa = 4.18NPMRR212 pKa = 11.84EE213 pKa = 4.33GEE215 pKa = 4.21NVTLSEE221 pKa = 5.11DD222 pKa = 3.95DD223 pKa = 3.59NTLVTGIQSDD233 pKa = 4.93PYY235 pKa = 9.5AIGYY239 pKa = 8.87FGYY242 pKa = 10.6AYY244 pKa = 10.42YY245 pKa = 10.99AEE247 pKa = 4.46NQDD250 pKa = 3.67TLKK253 pKa = 11.13VLGIDD258 pKa = 3.79NGDD261 pKa = 3.75GNPVKK266 pKa = 9.6PTGDD270 pKa = 3.82TIQDD274 pKa = 3.31GSYY277 pKa = 10.78SPLSRR282 pKa = 11.84PLFTYY287 pKa = 10.86VNVDD291 pKa = 3.29YY292 pKa = 9.99MKK294 pKa = 10.58EE295 pKa = 3.96KK296 pKa = 10.32PQVLDD301 pKa = 3.82FVKK304 pKa = 10.65FSIEE308 pKa = 3.9NAGAAAEE315 pKa = 4.16EE316 pKa = 4.37VGYY319 pKa = 10.72VALPDD324 pKa = 3.56EE325 pKa = 5.32KK326 pKa = 11.02YY327 pKa = 10.01QEE329 pKa = 3.91QLTEE333 pKa = 4.01IEE335 pKa = 4.19EE336 pKa = 4.25LAAEE340 pKa = 4.21

Molecular weight: 36.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317L4B9|A0A317L4B9_9BACI DUF2935 domain-containing protein OS=Gracilibacillus dipsosauri OX=178340 GN=DLJ74_00500 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.66NGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.38KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3941

0

3941

1208377

24

2921

306.6

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.513 ± 0.043

0.657 ± 0.011

5.347 ± 0.032

7.636 ± 0.044

4.603 ± 0.032

6.484 ± 0.036

2.185 ± 0.018

8.276 ± 0.043

6.808 ± 0.033

9.726 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.018

4.667 ± 0.03

3.544 ± 0.021

4.087 ± 0.031

3.802 ± 0.028

5.941 ± 0.028

5.435 ± 0.03

6.605 ± 0.03

1.188 ± 0.017

3.747 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski