Thermus phage phiLo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7URQ0|A0A3S7URQ0_9CAUD Uncharacterized protein OS=Thermus phage phiLo OX=2301536 GN=phiLo_115 PE=4 SV=1
MM1 pKa = 6.91MEE3 pKa = 4.11AMEE6 pKa = 4.58SLSEE10 pKa = 4.17LFFEE14 pKa = 5.07GTAEE18 pKa = 3.75EE19 pKa = 5.07SYY21 pKa = 11.42GNEE24 pKa = 3.65PTEE27 pKa = 4.04EE28 pKa = 4.16EE29 pKa = 4.12EE30 pKa = 5.68GVFLDD35 pKa = 4.29SLYY38 pKa = 11.3DD39 pKa = 3.57LTLGDD44 pKa = 3.78GLKK47 pKa = 10.71NKK49 pKa = 9.8VPIFFTKK56 pKa = 10.42SCWLEE61 pKa = 3.75ANKK64 pKa = 10.0ISPIGLGDD72 pKa = 4.3GYY74 pKa = 10.88LLPVSLVPVLSSNGTRR90 pKa = 11.84GVLYY94 pKa = 10.37SIYY97 pKa = 9.69TRR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84MTFPVFVQLNNLYY114 pKa = 10.25SSEE117 pKa = 3.98AVFVPLSPEE126 pKa = 3.73EE127 pKa = 4.06FGVSLEE133 pKa = 4.0EE134 pKa = 4.25DD135 pKa = 3.44RR136 pKa = 11.84EE137 pKa = 4.1EE138 pKa = 4.49GSFVFFKK145 pKa = 10.49AYY147 pKa = 10.18SSEE150 pKa = 4.13EE151 pKa = 3.88VEE153 pKa = 4.84APDD156 pKa = 4.68IYY158 pKa = 11.17GFYY161 pKa = 9.94PWDD164 pKa = 3.67VSHH167 pKa = 7.15LPTAISLFYY176 pKa = 10.21RR177 pKa = 11.84GRR179 pKa = 11.84IGKK182 pKa = 8.72EE183 pKa = 4.15GPVSLLLTPDD193 pKa = 3.66FVEE196 pKa = 4.6LNGGIPKK203 pKa = 9.65VIRR206 pKa = 11.84MIQGYY211 pKa = 7.05EE212 pKa = 3.92TLGMQVLISFGEE224 pKa = 3.93FFYY227 pKa = 11.15EE228 pKa = 4.24DD229 pKa = 3.7DD230 pKa = 4.01EE231 pKa = 4.73EE232 pKa = 4.8EE233 pKa = 4.28EE234 pKa = 4.7EE235 pKa = 4.9EE236 pKa = 5.45DD237 pKa = 4.97LLLL240 pKa = 5.53

Molecular weight: 27.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7URX0|A0A3S7URX0_9CAUD Uncharacterized protein OS=Thermus phage phiLo OX=2301536 GN=phiLo_151 PE=4 SV=1
MM1 pKa = 6.67TARR4 pKa = 11.84VRR6 pKa = 11.84TEE8 pKa = 3.26IEE10 pKa = 3.57IRR12 pKa = 11.84KK13 pKa = 9.22HH14 pKa = 4.31SRR16 pKa = 11.84KK17 pKa = 9.99NGNLEE22 pKa = 4.02SIYY25 pKa = 10.65TVLLDD30 pKa = 5.21LPLDD34 pKa = 3.88SSNSFSSLFRR44 pKa = 11.84KK45 pKa = 8.2TGALVYY51 pKa = 9.28CSCPDD56 pKa = 3.35FKK58 pKa = 11.19FRR60 pKa = 11.84LAYY63 pKa = 10.0ALRR66 pKa = 11.84INNLLIAKK74 pKa = 9.92RR75 pKa = 11.84SALGRR80 pKa = 11.84AFYY83 pKa = 10.05QFPRR87 pKa = 11.84ITNPRR92 pKa = 11.84LKK94 pKa = 10.63VRR96 pKa = 11.84ACKK99 pKa = 10.08HH100 pKa = 3.43ITMAVEE106 pKa = 4.17ALRR109 pKa = 11.84GRR111 pKa = 11.84TVTEE115 pKa = 4.14MVEE118 pKa = 4.08STARR122 pKa = 11.84GIPLFLTYY130 pKa = 10.97

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

53151

43

1724

320.2

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.368 ± 0.143

0.504 ± 0.05

5.215 ± 0.231

8.421 ± 0.241

4.702 ± 0.142

7.257 ± 0.192

2.002 ± 0.234

5.102 ± 0.112

6.323 ± 0.177

10.301 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.62 ± 0.069

3.308 ± 0.093

5.14 ± 0.164

2.468 ± 0.113

6.107 ± 0.18

8.412 ± 0.222

4.188 ± 0.166

7.748 ± 0.155

1.293 ± 0.067

4.521 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski