Streptococcus phage Javan93
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6ANS0|A0A4D6ANS0_9CAUD Tail length tape-measure protein OS=Streptococcus phage Javan93 OX=2548316 GN=Javan93_0034 PE=4 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.02 LVKK5 pKa = 10.02 TIQIGDD11 pKa = 4.56 DD12 pKa = 3.37 IYY14 pKa = 11.61 LPIPDD19 pKa = 3.64 QFGIQEE25 pKa = 4.13 GQKK28 pKa = 10.56 FNLYY32 pKa = 9.72 QSNDD36 pKa = 3.16 GTLVLSPSDD45 pKa = 3.65 SKK47 pKa = 11.57 LSADD51 pKa = 3.9 YY52 pKa = 10.88 QSLSSDD58 pKa = 3.54 EE59 pKa = 4.4 QEE61 pKa = 4.3 TTVVEE66 pKa = 4.1 QATLDD71 pKa = 3.65 EE72 pKa = 4.6 VANSVLSRR80 pKa = 11.84 HH81 pKa = 6.06 LDD83 pKa = 3.29 AFKK86 pKa = 10.92 EE87 pKa = 4.0 LAEE90 pKa = 4.13
Molecular weight: 9.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.205
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.656
ProMoST 3.961
Dawson 3.834
Bjellqvist 4.05
Wikipedia 3.757
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.961
Patrickios 3.503
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A4D6ANR2|A0A4D6ANR2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan93 OX=2548316 GN=Javan93_0024 PE=4 SV=1
MM1 pKa = 7.74 PRR3 pKa = 11.84 RR4 pKa = 11.84 PSTPCKK10 pKa = 10.2 QHH12 pKa = 5.85 GCPNLVTYY20 pKa = 9.66 GNKK23 pKa = 10.06 YY24 pKa = 10.5 CDD26 pKa = 3.43 DD27 pKa = 3.88 HH28 pKa = 6.67 KK29 pKa = 11.53 ANHH32 pKa = 6.38 ALDD35 pKa = 3.9 VKK37 pKa = 8.95 STKK40 pKa = 10.63 AKK42 pKa = 10.28 GYY44 pKa = 7.37 NARR47 pKa = 11.84 WNKK50 pKa = 9.75 ARR52 pKa = 11.84 LRR54 pKa = 11.84 YY55 pKa = 9.61 LKK57 pKa = 10.27 LHH59 pKa = 6.54 PLCVYY64 pKa = 8.54 CQRR67 pKa = 11.84 KK68 pKa = 9.01 GRR70 pKa = 11.84 LTKK73 pKa = 10.07 ATVVDD78 pKa = 5.28 HH79 pKa = 6.23 ITPHH83 pKa = 7.14 RR84 pKa = 11.84 GDD86 pKa = 4.72 QDD88 pKa = 4.61 LFWNQSNWQALCKK101 pKa = 10.09 SCHH104 pKa = 5.64 DD105 pKa = 4.22 RR106 pKa = 11.84 KK107 pKa = 8.93 TKK109 pKa = 8.13 TTDD112 pKa = 2.64 RR113 pKa = 11.84 YY114 pKa = 10.3 VEE116 pKa = 3.73 YY117 pKa = 10.38 SYY119 pKa = 11.5 RR120 pKa = 11.84 FF121 pKa = 3.35
Molecular weight: 14.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.213
IPC2_protein 9.224
IPC_protein 9.18
Toseland 9.78
ProMoST 9.545
Dawson 10.028
Bjellqvist 9.78
Wikipedia 10.204
Rodwell 10.379
Grimsley 10.101
Solomon 10.072
Lehninger 10.028
Nozaki 9.94
DTASelect 9.736
Thurlkill 9.882
EMBOSS 10.204
Sillero 9.984
Patrickios 7.497
IPC_peptide 10.058
IPC2_peptide 8.785
IPC2.peptide.svr19 8.09
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
43
0
43
11832
37
1039
275.2
30.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.032 ± 0.48
0.955 ± 0.162
6.119 ± 0.367
7.142 ± 0.381
3.668 ± 0.203
6.863 ± 0.371
1.978 ± 0.164
5.646 ± 0.354
7.387 ± 0.277
9.263 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.265 ± 0.16
4.234 ± 0.18
3.093 ± 0.269
4.37 ± 0.274
4.572 ± 0.397
6.728 ± 0.424
7.032 ± 0.458
6.609 ± 0.277
1.403 ± 0.105
3.643 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here