Artemia melana sponge associated circular genome

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL75|A0A0K1RL75_9CIRC Putative spliced replication initiation protein OS=Artemia melana sponge associated circular genome OX=1692243 PE=4 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.19SRR5 pKa = 11.84NWIVTLNNPEE15 pKa = 4.79LDD17 pKa = 3.75VEE19 pKa = 4.87DD20 pKa = 3.73YY21 pKa = 11.69AFALKK26 pKa = 10.72LSGATWFTGQLEE38 pKa = 4.4KK39 pKa = 11.03GDD41 pKa = 3.71QGTVHH46 pKa = 6.56LQAAFGYY53 pKa = 9.62ANTVRR58 pKa = 11.84FSAVKK63 pKa = 10.21KK64 pKa = 9.88ILPEE68 pKa = 3.64AHH70 pKa = 6.84IEE72 pKa = 4.05KK73 pKa = 9.37TNCGASAAEE82 pKa = 4.25YY83 pKa = 10.12CSKK86 pKa = 11.12SDD88 pKa = 3.43TRR90 pKa = 11.84VSEE93 pKa = 3.94PHH95 pKa = 6.47SFGTPPVKK103 pKa = 10.45RR104 pKa = 11.84NLKK107 pKa = 9.52GNHH110 pKa = 5.61KK111 pKa = 10.32VRR113 pKa = 11.84TQQLLDD119 pKa = 3.41KK120 pKa = 10.27GALKK124 pKa = 10.61ALEE127 pKa = 5.27DD128 pKa = 3.86GDD130 pKa = 3.54ISLRR134 pKa = 11.84EE135 pKa = 3.93FKK137 pKa = 10.71LFKK140 pKa = 11.1ANIEE144 pKa = 3.86LAKK147 pKa = 10.86AEE149 pKa = 4.55FYY151 pKa = 10.63KK152 pKa = 10.12WWDD155 pKa = 3.58GYY157 pKa = 11.46NNQAVVIIDD166 pKa = 4.74DD167 pKa = 4.67LDD169 pKa = 4.93SDD171 pKa = 4.79ALGHH175 pKa = 6.32HH176 pKa = 6.52LKK178 pKa = 9.82IWADD182 pKa = 3.62HH183 pKa = 6.13YY184 pKa = 11.41PFIAEE189 pKa = 4.48FKK191 pKa = 10.78GGAQNIRR198 pKa = 11.84PHH200 pKa = 6.7KK201 pKa = 10.42IVVTSNYY208 pKa = 9.93SISEE212 pKa = 4.21LFSKK216 pKa = 9.99DD217 pKa = 3.12VKK219 pKa = 10.03MAEE222 pKa = 4.07AIARR226 pKa = 11.84RR227 pKa = 11.84FTT229 pKa = 3.39

Molecular weight: 25.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL75|A0A0K1RL75_9CIRC Putative spliced replication initiation protein OS=Artemia melana sponge associated circular genome OX=1692243 PE=4 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.19SRR5 pKa = 11.84NWIVTLNNPEE15 pKa = 4.79LDD17 pKa = 3.75VEE19 pKa = 4.87DD20 pKa = 3.73YY21 pKa = 11.69AFALKK26 pKa = 10.72LSGATWFTGQLEE38 pKa = 4.4KK39 pKa = 11.03GDD41 pKa = 3.71QGTVHH46 pKa = 6.56LQAAFGYY53 pKa = 9.62ANTVRR58 pKa = 11.84FSAVKK63 pKa = 10.21KK64 pKa = 9.88ILPEE68 pKa = 3.64AHH70 pKa = 6.84IEE72 pKa = 4.05KK73 pKa = 9.37TNCGASAAEE82 pKa = 4.25YY83 pKa = 10.12CSKK86 pKa = 11.12SDD88 pKa = 3.43TRR90 pKa = 11.84VSEE93 pKa = 3.94PHH95 pKa = 6.47SFGTPPVKK103 pKa = 10.45RR104 pKa = 11.84NLKK107 pKa = 9.52GNHH110 pKa = 5.61KK111 pKa = 10.32VRR113 pKa = 11.84TQQLLDD119 pKa = 3.41KK120 pKa = 10.27GALKK124 pKa = 10.61ALEE127 pKa = 5.27DD128 pKa = 3.86GDD130 pKa = 3.54ISLRR134 pKa = 11.84EE135 pKa = 3.93FKK137 pKa = 10.71LFKK140 pKa = 11.1ANIEE144 pKa = 3.86LAKK147 pKa = 10.86AEE149 pKa = 4.55FYY151 pKa = 10.63KK152 pKa = 10.12WWDD155 pKa = 3.58GYY157 pKa = 11.46NNQAVVIIDD166 pKa = 4.74DD167 pKa = 4.67LDD169 pKa = 4.93SDD171 pKa = 4.79ALGHH175 pKa = 6.32HH176 pKa = 6.52LKK178 pKa = 9.82IWADD182 pKa = 3.62HH183 pKa = 6.13YY184 pKa = 11.41PFIAEE189 pKa = 4.48FKK191 pKa = 10.78GGAQNIRR198 pKa = 11.84PHH200 pKa = 6.7KK201 pKa = 10.42IVVTSNYY208 pKa = 9.93SISEE212 pKa = 4.21LFSKK216 pKa = 9.99DD217 pKa = 3.12VKK219 pKa = 10.03MAEE222 pKa = 4.07AIARR226 pKa = 11.84RR227 pKa = 11.84FTT229 pKa = 3.39

Molecular weight: 25.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

229

229

229

229.0

25.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.48 ± 0.0

0.873 ± 0.0

6.114 ± 0.0

6.114 ± 0.0

5.24 ± 0.0

6.114 ± 0.0

3.493 ± 0.0

5.677 ± 0.0

9.17 ± 0.0

8.297 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.873 ± 0.0

5.24 ± 0.0

3.057 ± 0.0

3.057 ± 0.0

3.93 ± 0.0

6.55 ± 0.0

4.803 ± 0.0

5.677 ± 0.0

2.183 ± 0.0

3.057 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski