Prevotella sp. tc2-28

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2895 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3X6U2|A0A1H3X6U2_9BACT tRNA pseudouridine synthase B OS=Prevotella sp. tc2-28 OX=1761888 GN=truB PE=3 SV=1
MM1 pKa = 7.46AKK3 pKa = 9.97VLEE6 pKa = 4.18GLYY9 pKa = 8.9YY10 pKa = 10.0TEE12 pKa = 3.42SHH14 pKa = 6.67EE15 pKa = 4.22YY16 pKa = 10.53VKK18 pKa = 11.17VEE20 pKa = 3.88GNIGYY25 pKa = 10.19VGITDD30 pKa = 3.99YY31 pKa = 11.29AQHH34 pKa = 6.72ALGNVVYY41 pKa = 11.1VDD43 pKa = 3.98MPDD46 pKa = 3.27VDD48 pKa = 5.32DD49 pKa = 4.44EE50 pKa = 4.85VSAGEE55 pKa = 3.87EE56 pKa = 3.95FGAVEE61 pKa = 4.31SVKK64 pKa = 10.6AASDD68 pKa = 4.46LISPVTGVVLEE79 pKa = 4.26VNEE82 pKa = 4.11ALEE85 pKa = 4.85DD86 pKa = 3.69EE87 pKa = 4.72PEE89 pKa = 4.8RR90 pKa = 11.84INADD94 pKa = 3.17AYY96 pKa = 9.6EE97 pKa = 3.85NWIIKK102 pKa = 10.12VEE104 pKa = 3.96LSDD107 pKa = 4.71KK108 pKa = 11.6GEE110 pKa = 4.48LKK112 pKa = 11.05NLMDD116 pKa = 4.46AKK118 pKa = 10.71AYY120 pKa = 9.84TEE122 pKa = 4.08FCEE125 pKa = 4.22QQ126 pKa = 3.23

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4BJE5|A0A1H4BJE5_9BACT Iron complex transport system permease protein OS=Prevotella sp. tc2-28 OX=1761888 GN=SAMN04487851_10714 PE=3 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2895

0

2895

1038832

28

3873

358.8

40.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.36 ± 0.041

1.347 ± 0.019

5.878 ± 0.028

6.276 ± 0.036

4.256 ± 0.028

7.009 ± 0.049

2.136 ± 0.025

6.512 ± 0.033

6.317 ± 0.04

8.741 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.042 ± 0.023

4.826 ± 0.037

3.694 ± 0.023

4.003 ± 0.034

4.77 ± 0.032

5.69 ± 0.032

5.822 ± 0.044

6.644 ± 0.033

1.371 ± 0.017

4.307 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski