Bacillus sp. V59.32b

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E2JVZ8|A0A3E2JVZ8_9BACI Tyrosine--tRNA ligase OS=Bacillus sp. V59.32b OX=1758642 GN=tyrS PE=3 SV=1
MM1 pKa = 7.36TIRR4 pKa = 11.84WVCWTWITISSVTQTPLFSDD24 pKa = 3.67YY25 pKa = 11.45DD26 pKa = 3.92GDD28 pKa = 4.2GFSDD32 pKa = 4.01PFDD35 pKa = 5.45AINDD39 pKa = 3.4IDD41 pKa = 5.08GDD43 pKa = 4.1FTNDD47 pKa = 3.18ANDD50 pKa = 3.91PFIDD54 pKa = 3.62TNGNHH59 pKa = 5.85IHH61 pKa = 7.52DD62 pKa = 4.99GNDD65 pKa = 3.28PFQDD69 pKa = 3.29WDD71 pKa = 3.42QDD73 pKa = 3.78GLFDD77 pKa = 4.51GGYY80 pKa = 7.4MAYY83 pKa = 10.04RR84 pKa = 11.84KK85 pKa = 10.25KK86 pKa = 10.79LVTQFASLGSVSYY99 pKa = 10.33EE100 pKa = 3.75

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E2JNE1|A0A3E2JNE1_9BACI Imidazole glycerol phosphate synthase subunit HisF OS=Bacillus sp. V59.32b OX=1758642 GN=hisF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.83NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3615

0

3615

1020100

16

2286

282.2

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.16 ± 0.045

0.704 ± 0.013

5.194 ± 0.031

7.662 ± 0.049

4.491 ± 0.037

7.061 ± 0.043

2.049 ± 0.02

7.859 ± 0.043

7.018 ± 0.04

9.615 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.021

4.386 ± 0.031

3.655 ± 0.025

3.582 ± 0.026

4.12 ± 0.035

6.085 ± 0.036

5.332 ± 0.034

6.863 ± 0.032

0.977 ± 0.017

3.419 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski