Gammapapillomavirus 16
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AM05|A0A2D2AM05_9PAPI Minor capsid protein L2 OS=Gammapapillomavirus 16 OX=1513261 GN=L2 PE=3 SV=1
MM1 pKa = 7.35 QGRR4 pKa = 11.84 KK5 pKa = 5.03 RR6 pKa = 11.84 TKK8 pKa = 8.65 RR9 pKa = 11.84 ASVEE13 pKa = 3.94 DD14 pKa = 4.57 IYY16 pKa = 11.72 AKK18 pKa = 10.8 GCTQPGGYY26 pKa = 9.78 CPPDD30 pKa = 3.29 VKK32 pKa = 11.21 NKK34 pKa = 10.1 VEE36 pKa = 4.13 GNTWADD42 pKa = 3.44 WILKK46 pKa = 9.19 VFGSVVYY53 pKa = 10.04 FGGLGIGTGKK63 pKa = 8.78 GTGGSTGYY71 pKa = 8.91 TPLGGVGGSRR81 pKa = 11.84 GPTTSIRR88 pKa = 11.84 PTVPIEE94 pKa = 4.26 TIGLPDD100 pKa = 3.67 IVTVNPVEE108 pKa = 4.56 PGASSIVPLAEE119 pKa = 4.28 GLPEE123 pKa = 3.99 PAVIDD128 pKa = 3.72 TGSSIPGLAADD139 pKa = 4.11 NTNITTVIDD148 pKa = 3.86 PLSEE152 pKa = 3.98 VTGLGEE158 pKa = 4.13 HH159 pKa = 7.12 PNLIGATSEE168 pKa = 4.04 DD169 pKa = 3.97 TVAVLDD175 pKa = 4.31 VQTSPPPAKK184 pKa = 10.25 KK185 pKa = 9.97 ILLDD189 pKa = 3.56 TSLTDD194 pKa = 3.01 TTFRR198 pKa = 11.84 VDD200 pKa = 2.89 THH202 pKa = 6.75 ASHH205 pKa = 7.36 VDD207 pKa = 2.95 ANLNIFVDD215 pKa = 3.95 AQSFGSHH222 pKa = 6.29 IGLSEE227 pKa = 5.26 AIPLQEE233 pKa = 4.28 INLISEE239 pKa = 4.42 FEE241 pKa = 4.02 IEE243 pKa = 4.27 EE244 pKa = 5.1 PIPSTSTPIAEE255 pKa = 4.04 RR256 pKa = 11.84 VVTRR260 pKa = 11.84 AKK262 pKa = 10.21 QLYY265 pKa = 8.21 NRR267 pKa = 11.84 YY268 pKa = 8.13 VQQVPTRR275 pKa = 11.84 PAEE278 pKa = 3.8 FATYY282 pKa = 10.17 SSRR285 pKa = 11.84 FEE287 pKa = 4.22 FEE289 pKa = 4.26 NPAFMKK295 pKa = 10.57 DD296 pKa = 3.18 VTLEE300 pKa = 3.98 FEE302 pKa = 4.17 NDD304 pKa = 3.18 LAEE307 pKa = 4.54 IEE309 pKa = 4.83 QITAPAISDD318 pKa = 3.35 VRR320 pKa = 11.84 ILNRR324 pKa = 11.84 PTFSEE329 pKa = 4.27 TTDD332 pKa = 3.06 RR333 pKa = 11.84 TVRR336 pKa = 11.84 ISRR339 pKa = 11.84 LGQRR343 pKa = 11.84 AGMKK347 pKa = 8.73 TRR349 pKa = 11.84 SGLDD353 pKa = 2.89 VGQRR357 pKa = 11.84 VHH359 pKa = 7.29 FYY361 pKa = 10.68 YY362 pKa = 10.56 DD363 pKa = 3.16 VSKK366 pKa = 10.71 ISSTIEE372 pKa = 3.5 MNTFGEE378 pKa = 4.53 YY379 pKa = 9.55 SHH381 pKa = 7.06 EE382 pKa = 4.1 STIVDD387 pKa = 3.77 EE388 pKa = 4.8 LLSSSFINPFEE399 pKa = 4.14 MPIEE403 pKa = 5.08 DD404 pKa = 3.64 IFSDD408 pKa = 3.95 TEE410 pKa = 4.57 LIDD413 pKa = 3.97 PLEE416 pKa = 4.03 EE417 pKa = 4.26 DD418 pKa = 4.15 FRR420 pKa = 11.84 NSHH423 pKa = 7.21 LILPFSDD430 pKa = 3.98 DD431 pKa = 3.13 QDD433 pKa = 4.14 VYY435 pKa = 11.64 ALPTLPPGIGLKK447 pKa = 10.5 LYY449 pKa = 10.32 TDD451 pKa = 3.98 LTEE454 pKa = 4.71 KK455 pKa = 10.85 DD456 pKa = 4.06 LFIHH460 pKa = 6.98 HH461 pKa = 6.98 PTQNTQIIVPDD472 pKa = 3.6 SPYY475 pKa = 10.53 IPLQPPIGAVIDD487 pKa = 3.6 NDD489 pKa = 4.29 DD490 pKa = 4.21 YY491 pKa = 11.81 YY492 pKa = 11.67 LHH494 pKa = 7.46 PSLLTRR500 pKa = 11.84 KK501 pKa = 9.71 RR502 pKa = 11.84 KK503 pKa = 9.33 RR504 pKa = 11.84 AFFF507 pKa = 4.42
Molecular weight: 55.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.668
IPC2_protein 4.635
IPC_protein 4.596
Toseland 4.457
ProMoST 4.711
Dawson 4.558
Bjellqvist 4.698
Wikipedia 4.431
Rodwell 4.444
Grimsley 4.368
Solomon 4.558
Lehninger 4.507
Nozaki 4.66
DTASelect 4.838
Thurlkill 4.457
EMBOSS 4.457
Sillero 4.724
Patrickios 3.923
IPC_peptide 4.558
IPC2_peptide 4.711
IPC2.peptide.svr19 4.66
Protein with the highest isoelectric point:
>tr|A0A2D2ALU7|A0A2D2ALU7_9PAPI Protein E7 OS=Gammapapillomavirus 16 OX=1513261 GN=E7 PE=3 SV=1
MM1 pKa = 7.6 EE2 pKa = 5.86 PLFPTKK8 pKa = 10.52 LNEE11 pKa = 3.72 YY12 pKa = 9.87 CSYY15 pKa = 11.32 FDD17 pKa = 4.62 ISFFDD22 pKa = 4.64 LSLKK26 pKa = 10.8 CIFCKK31 pKa = 10.37 HH32 pKa = 5.4 YY33 pKa = 11.28 LNLVDD38 pKa = 5.35 LAYY41 pKa = 10.0 FYY43 pKa = 11.23 EE44 pKa = 5.27 KK45 pKa = 10.7 GLSLVWRR52 pKa = 11.84 NNICYY57 pKa = 9.82 ACCDD61 pKa = 3.41 RR62 pKa = 11.84 CLKK65 pKa = 10.72 CSARR69 pKa = 11.84 YY70 pKa = 7.05 EE71 pKa = 4.36 ANRR74 pKa = 11.84 HH75 pKa = 4.58 FQCTFNTATLHH86 pKa = 6.64 AVLEE90 pKa = 4.54 KK91 pKa = 10.1 PLQDD95 pKa = 3.29 IEE97 pKa = 4.14 IRR99 pKa = 11.84 CYY101 pKa = 10.45 YY102 pKa = 9.24 CLCILSLVEE111 pKa = 4.08 KK112 pKa = 10.13 CDD114 pKa = 4.3 LIARR118 pKa = 11.84 GRR120 pKa = 11.84 PTCLVRR126 pKa = 11.84 GYY128 pKa = 10.43 FRR130 pKa = 11.84 APCKK134 pKa = 10.39 DD135 pKa = 3.55 CIRR138 pKa = 11.84 RR139 pKa = 11.84 EE140 pKa = 3.95 IYY142 pKa = 10.51
Molecular weight: 16.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.231
IPC2_protein 7.263
IPC_protein 7.088
Toseland 6.547
ProMoST 7.79
Dawson 7.775
Bjellqvist 8.302
Wikipedia 7.673
Rodwell 7.79
Grimsley 6.532
Solomon 7.819
Lehninger 7.849
Nozaki 8.624
DTASelect 7.936
Thurlkill 7.98
EMBOSS 7.995
Sillero 8.375
Patrickios 0.515
IPC_peptide 7.819
IPC2_peptide 7.878
IPC2.peptide.svr19 7.738
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2395
99
610
342.1
38.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.887 ± 0.251
2.38 ± 0.882
6.221 ± 0.586
6.681 ± 0.726
4.301 ± 0.548
5.261 ± 0.747
2.296 ± 0.239
5.428 ± 1.025
5.219 ± 0.679
9.812 ± 0.657
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.837 ± 0.361
5.762 ± 0.647
5.846 ± 1.012
3.967 ± 0.38
5.386 ± 0.823
7.307 ± 0.607
6.472 ± 0.784
5.01 ± 0.644
1.211 ± 0.296
3.716 ± 0.522
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here