Treponema sp. J25

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; unclassified Treponema

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R4BPR0|A0A4R4BPR0_9SPIO Uncharacterized protein OS=Treponema sp. J25 OX=2094121 GN=C5O22_06565 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.23KK3 pKa = 10.15YY4 pKa = 10.59VCDD7 pKa = 3.52VCGYY11 pKa = 10.04IYY13 pKa = 10.6DD14 pKa = 4.4PAAGDD19 pKa = 3.89PDD21 pKa = 4.18NGVAPGTPFEE31 pKa = 4.91KK32 pKa = 10.82LPADD36 pKa = 4.46WVCPMCGAGKK46 pKa = 10.27DD47 pKa = 3.57DD48 pKa = 4.86FSPVEE53 pKa = 3.81

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R4BMM9|A0A4R4BMM9_9SPIO Uncharacterized protein OS=Treponema sp. J25 OX=2094121 GN=C5O22_11005 PE=4 SV=1
MM1 pKa = 7.52NSFFTLVFEE10 pKa = 4.94GDD12 pKa = 3.1RR13 pKa = 11.84SEE15 pKa = 5.27NYY17 pKa = 9.04VDD19 pKa = 3.59SEE21 pKa = 4.5GVHH24 pKa = 6.53RR25 pKa = 11.84KK26 pKa = 7.22TAGQNNTVSAGLIGGVVAAPPWRR49 pKa = 11.84GGCARR54 pKa = 11.84AGPGRR59 pKa = 11.84GRR61 pKa = 11.84CGGVPRR67 pKa = 11.84RR68 pKa = 11.84GGSGGRR74 pKa = 11.84QRR76 pKa = 11.84ACYY79 pKa = 9.21AARR82 pKa = 11.84AAEE85 pKa = 4.31GAADD89 pKa = 3.76NHH91 pKa = 6.4GEE93 pKa = 3.92APRR96 pKa = 11.84RR97 pKa = 11.84ITGPQWPAMFPRR109 pKa = 11.84WQPEE113 pKa = 3.83PLGKK117 pKa = 9.36WLRR120 pKa = 11.84RR121 pKa = 11.84PEE123 pKa = 4.15RR124 pKa = 11.84GGDD127 pKa = 3.6FPLLEE132 pKa = 5.2FGNHH136 pKa = 6.06WIAVV140 pKa = 3.69

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2661

0

2661

953465

37

3796

358.3

40.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.9 ± 0.054

0.939 ± 0.014

4.63 ± 0.031

7.205 ± 0.05

4.447 ± 0.04

7.555 ± 0.043

1.835 ± 0.023

6.654 ± 0.04

5.138 ± 0.043

10.647 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.019

3.275 ± 0.032

4.882 ± 0.033

3.796 ± 0.031

6.06 ± 0.049

6.126 ± 0.038

5.222 ± 0.044

6.672 ± 0.038

1.33 ± 0.019

3.606 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski