Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) (Clostridium thermosaccharolyticum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacterales Family III. Incertae Sedis; Thermoanaerobacterium; Thermoanaerobacterium thermosaccharolyticum

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2583 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9TLJ2|D9TLJ2_THETC Alpha amylase catalytic region OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=Tthe_0763 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.14KK3 pKa = 9.49WQCTVCGYY11 pKa = 10.31IYY13 pKa = 10.4DD14 pKa = 4.39PEE16 pKa = 5.2VGDD19 pKa = 3.84PTQNIPPGTKK29 pKa = 9.93FEE31 pKa = 5.16DD32 pKa = 4.63LPDD35 pKa = 3.8DD36 pKa = 4.48WVCPDD41 pKa = 4.09CGVGKK46 pKa = 10.0DD47 pKa = 3.52QFEE50 pKa = 4.7KK51 pKa = 10.62II52 pKa = 3.9

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9TRR3|D9TRR3_THETC Polar amino acid ABC transporter inner membrane subunit OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=Tthe_2175 PE=3 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84THH5 pKa = 5.51QPKK8 pKa = 9.39VRR10 pKa = 11.84HH11 pKa = 5.93RR12 pKa = 11.84KK13 pKa = 9.12KK14 pKa = 9.6EE15 pKa = 3.48HH16 pKa = 5.74GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTKK25 pKa = 10.14NGRR28 pKa = 11.84NVLKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.24GRR39 pKa = 11.84HH40 pKa = 5.43RR41 pKa = 11.84LTAA44 pKa = 4.06

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2583

0

2583

784648

30

2874

303.8

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.997 ± 0.052

0.934 ± 0.02

6.212 ± 0.04

6.658 ± 0.057

4.185 ± 0.042

6.631 ± 0.049

1.386 ± 0.018

9.968 ± 0.062

8.638 ± 0.049

9.037 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.02

5.816 ± 0.044

3.206 ± 0.027

2.282 ± 0.028

3.687 ± 0.033

6.094 ± 0.038

4.751 ± 0.036

6.822 ± 0.04

0.734 ± 0.019

4.264 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski