Goose circovirus (GoCV)
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8AYV0|Q8AYV0_GOCV ATP-dependent helicase Rep OS=Goose circovirus OX=146032 GN=rep PE=3 SV=1
MM1 pKa = 7.77 AKK3 pKa = 10.18 NGNYY7 pKa = 9.84 SYY9 pKa = 10.84 KK10 pKa = 10.27 RR11 pKa = 11.84 WVFTINNPTFEE22 pKa = 5.2 DD23 pKa = 4.07 YY24 pKa = 11.18 CSVVEE29 pKa = 5.51 FCNLDD34 pKa = 3.26 NCKK37 pKa = 9.84 FAIVGEE43 pKa = 4.22 EE44 pKa = 3.9 KK45 pKa = 10.83 GEE47 pKa = 4.24 KK48 pKa = 10.01 EE49 pKa = 4.29 GTPHH53 pKa = 6.51 LQGFLSLRR61 pKa = 11.84 KK62 pKa = 8.91 NAKK65 pKa = 9.52 AAALEE70 pKa = 4.18 EE71 pKa = 4.34 KK72 pKa = 10.67 LGGRR76 pKa = 11.84 AWLSRR81 pKa = 11.84 ARR83 pKa = 11.84 GSDD86 pKa = 3.31 EE87 pKa = 5.6 DD88 pKa = 3.95 NEE90 pKa = 4.65 EE91 pKa = 4.28 YY92 pKa = 10.42 CSKK95 pKa = 10.57 EE96 pKa = 3.61 STYY99 pKa = 11.13 LRR101 pKa = 11.84 VGEE104 pKa = 4.16 PNRR107 pKa = 11.84 KK108 pKa = 8.88 GRR110 pKa = 11.84 SSDD113 pKa = 3.57 LSDD116 pKa = 3.62 AASDD120 pKa = 3.84 VLAGLPITDD129 pKa = 3.67 VARR132 pKa = 11.84 KK133 pKa = 9.64 YY134 pKa = 7.27 PTTYY138 pKa = 11.11 VMFGRR143 pKa = 11.84 GLEE146 pKa = 4.04 RR147 pKa = 11.84 LRR149 pKa = 11.84 QLIVEE154 pKa = 4.79 TARR157 pKa = 11.84 DD158 pKa = 3.5 WKK160 pKa = 10.01 TEE162 pKa = 3.9 VIVLIGRR169 pKa = 11.84 PGSGKK174 pKa = 9.9 SRR176 pKa = 11.84 YY177 pKa = 9.78 AFEE180 pKa = 4.04 FPARR184 pKa = 11.84 EE185 pKa = 3.95 KK186 pKa = 10.76 YY187 pKa = 9.98 YY188 pKa = 10.72 KK189 pKa = 10.38 SRR191 pKa = 11.84 GKK193 pKa = 8.8 WWDD196 pKa = 3.5 GYY198 pKa = 10.75 NGQDD202 pKa = 3.46 VVVMDD207 pKa = 5.79 DD208 pKa = 3.97 FYY210 pKa = 11.92 GWLPYY215 pKa = 10.72 DD216 pKa = 4.19 DD217 pKa = 5.71 LLRR220 pKa = 11.84 ICDD223 pKa = 4.02 RR224 pKa = 11.84 YY225 pKa = 9.66 PLRR228 pKa = 11.84 VEE230 pKa = 4.35 YY231 pKa = 10.33 KK232 pKa = 9.91 GGMTQFVAKK241 pKa = 9.46 TLIITSNRR249 pKa = 11.84 EE250 pKa = 3.15 PRR252 pKa = 11.84 DD253 pKa = 3.23 WYY255 pKa = 10.67 KK256 pKa = 11.37 CEE258 pKa = 4.01 FDD260 pKa = 3.79 VSALYY265 pKa = 10.38 RR266 pKa = 11.84 RR267 pKa = 11.84 INQYY271 pKa = 11.13 LILTPDD277 pKa = 3.85 GYY279 pKa = 11.67 APAPEE284 pKa = 3.96 FMLPFKK290 pKa = 10.73 INYY293 pKa = 8.42
Molecular weight: 33.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.09
IPC2_protein 7.483
IPC_protein 7.424
Toseland 6.927
ProMoST 8.068
Dawson 8.097
Bjellqvist 8.361
Wikipedia 8.024
Rodwell 8.097
Grimsley 6.868
Solomon 8.185
Lehninger 8.2
Nozaki 8.463
DTASelect 8.156
Thurlkill 8.2
EMBOSS 8.302
Sillero 8.507
Patrickios 4.113
IPC_peptide 8.185
IPC2_peptide 7.337
IPC2.peptide.svr19 7.677
Protein with the highest isoelectric point:
>tr|Q8AYV0|Q8AYV0_GOCV ATP-dependent helicase Rep OS=Goose circovirus OX=146032 GN=rep PE=3 SV=1
MM1 pKa = 7.77 AKK3 pKa = 10.18 NGNYY7 pKa = 9.84 SYY9 pKa = 10.84 KK10 pKa = 10.27 RR11 pKa = 11.84 WVFTINNPTFEE22 pKa = 5.2 DD23 pKa = 4.07 YY24 pKa = 11.18 CSVVEE29 pKa = 5.51 FCNLDD34 pKa = 3.26 NCKK37 pKa = 9.84 FAIVGEE43 pKa = 4.22 EE44 pKa = 3.9 KK45 pKa = 10.83 GEE47 pKa = 4.24 KK48 pKa = 10.01 EE49 pKa = 4.29 GTPHH53 pKa = 6.51 LQGFLSLRR61 pKa = 11.84 KK62 pKa = 8.91 NAKK65 pKa = 9.52 AAALEE70 pKa = 4.18 EE71 pKa = 4.34 KK72 pKa = 10.67 LGGRR76 pKa = 11.84 AWLSRR81 pKa = 11.84 ARR83 pKa = 11.84 GSDD86 pKa = 3.31 EE87 pKa = 5.6 DD88 pKa = 3.95 NEE90 pKa = 4.65 EE91 pKa = 4.28 YY92 pKa = 10.42 CSKK95 pKa = 10.57 EE96 pKa = 3.61 STYY99 pKa = 11.13 LRR101 pKa = 11.84 VGEE104 pKa = 4.16 PNRR107 pKa = 11.84 KK108 pKa = 8.88 GRR110 pKa = 11.84 SSDD113 pKa = 3.57 LSDD116 pKa = 3.62 AASDD120 pKa = 3.84 VLAGLPITDD129 pKa = 3.67 VARR132 pKa = 11.84 KK133 pKa = 9.64 YY134 pKa = 7.27 PTTYY138 pKa = 11.11 VMFGRR143 pKa = 11.84 GLEE146 pKa = 4.04 RR147 pKa = 11.84 LRR149 pKa = 11.84 QLIVEE154 pKa = 4.79 TARR157 pKa = 11.84 DD158 pKa = 3.5 WKK160 pKa = 10.01 TEE162 pKa = 3.9 VIVLIGRR169 pKa = 11.84 PGSGKK174 pKa = 9.9 SRR176 pKa = 11.84 YY177 pKa = 9.78 AFEE180 pKa = 4.04 FPARR184 pKa = 11.84 EE185 pKa = 3.95 KK186 pKa = 10.76 YY187 pKa = 9.98 YY188 pKa = 10.72 KK189 pKa = 10.38 SRR191 pKa = 11.84 GKK193 pKa = 8.8 WWDD196 pKa = 3.5 GYY198 pKa = 10.75 NGQDD202 pKa = 3.46 VVVMDD207 pKa = 5.79 DD208 pKa = 3.97 FYY210 pKa = 11.92 GWLPYY215 pKa = 10.72 DD216 pKa = 4.19 DD217 pKa = 5.71 LLRR220 pKa = 11.84 ICDD223 pKa = 4.02 RR224 pKa = 11.84 YY225 pKa = 9.66 PLRR228 pKa = 11.84 VEE230 pKa = 4.35 YY231 pKa = 10.33 KK232 pKa = 9.91 GGMTQFVAKK241 pKa = 9.46 TLIITSNRR249 pKa = 11.84 EE250 pKa = 3.15 PRR252 pKa = 11.84 DD253 pKa = 3.23 WYY255 pKa = 10.67 KK256 pKa = 11.37 CEE258 pKa = 4.01 FDD260 pKa = 3.79 VSALYY265 pKa = 10.38 RR266 pKa = 11.84 RR267 pKa = 11.84 INQYY271 pKa = 11.13 LILTPDD277 pKa = 3.85 GYY279 pKa = 11.67 APAPEE284 pKa = 3.96 FMLPFKK290 pKa = 10.73 INYY293 pKa = 8.42
Molecular weight: 33.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.09
IPC2_protein 7.483
IPC_protein 7.424
Toseland 6.927
ProMoST 8.068
Dawson 8.097
Bjellqvist 8.361
Wikipedia 8.024
Rodwell 8.097
Grimsley 6.868
Solomon 8.185
Lehninger 8.2
Nozaki 8.463
DTASelect 8.156
Thurlkill 8.2
EMBOSS 8.302
Sillero 8.507
Patrickios 4.113
IPC_peptide 8.185
IPC2_peptide 7.337
IPC2.peptide.svr19 7.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
293
293
293
293.0
33.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.485 ± 0.0
2.048 ± 0.0
6.485 ± 0.0
7.509 ± 0.0
4.437 ± 0.0
7.85 ± 0.0
0.341 ± 0.0
4.096 ± 0.0
6.826 ± 0.0
8.191 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.706 ± 0.0
4.437 ± 0.0
4.778 ± 0.0
1.706 ± 0.0
8.191 ± 0.0
5.461 ± 0.0
4.437 ± 0.0
5.802 ± 0.0
2.389 ± 0.0
6.826 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here