Porcine epidemic diarrhea virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Pedacovirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059VJE6|A0A059VJE6_9ALPC 3C-like proteinase OS=Porcine epidemic diarrhea virus OX=28295 GN=ORF1a PE=3 SV=1
MM1 pKa = 7.99KK2 pKa = 10.42SLTYY6 pKa = 9.83FWLFLPVLSTLSLPQDD22 pKa = 3.57VTRR25 pKa = 11.84CSANTNFRR33 pKa = 11.84RR34 pKa = 11.84FFSKK38 pKa = 10.72FNVQAPAVVVLGGYY52 pKa = 10.04LPIGEE57 pKa = 4.23NQGVNSTWYY66 pKa = 10.45CAGQHH71 pKa = 5.25PTASGVHH78 pKa = 6.7GIFVSHH84 pKa = 6.55IRR86 pKa = 11.84GGHH89 pKa = 4.59GFEE92 pKa = 4.58IGISQEE98 pKa = 3.85PFDD101 pKa = 4.04PSGYY105 pKa = 9.89QLYY108 pKa = 10.0LHH110 pKa = 7.06KK111 pKa = 10.35ATNGNTNATARR122 pKa = 11.84LRR124 pKa = 11.84ICQFPSIKK132 pKa = 9.87TLGPTANNDD141 pKa = 2.87VTTGRR146 pKa = 11.84NCLFNKK152 pKa = 9.98AIPAHH157 pKa = 5.62MSEE160 pKa = 4.46HH161 pKa = 5.85SVVGITWDD169 pKa = 3.48NDD171 pKa = 3.08RR172 pKa = 11.84VTVFSDD178 pKa = 3.69KK179 pKa = 10.46IYY181 pKa = 11.02YY182 pKa = 10.22FYY184 pKa = 10.98FKK186 pKa = 10.95NDD188 pKa = 3.08WSRR191 pKa = 11.84VATKK195 pKa = 10.01CYY197 pKa = 10.67NSGGCAMQYY206 pKa = 10.42VYY208 pKa = 11.04EE209 pKa = 4.08PTYY212 pKa = 11.64YY213 pKa = 9.87MLNVTSAGEE222 pKa = 4.14DD223 pKa = 3.74GISYY227 pKa = 9.02QPCTANCIGYY237 pKa = 9.17AANVFATEE245 pKa = 4.27PNGHH249 pKa = 6.21IPEE252 pKa = 4.55GFSFNNWFLLSNDD265 pKa = 3.05STLVHH270 pKa = 6.24GKK272 pKa = 9.44VVSNQPLLVNCLLAIPKK289 pKa = 9.74IYY291 pKa = 10.87GLGQFFSFNQTIDD304 pKa = 3.8GVCNGAAVQRR314 pKa = 11.84APEE317 pKa = 3.93ALRR320 pKa = 11.84FNINDD325 pKa = 3.42TSVILAEE332 pKa = 4.36GSIVLHH338 pKa = 6.0TALGTNFSFVCSNSSNPHH356 pKa = 6.09LATFAIPLGATQVPYY371 pKa = 11.25YY372 pKa = 10.63CFLKK376 pKa = 10.57VDD378 pKa = 4.51TYY380 pKa = 11.79NSTVYY385 pKa = 10.65KK386 pKa = 9.87FLAVLPPTVRR396 pKa = 11.84EE397 pKa = 3.97IVITKK402 pKa = 10.54YY403 pKa = 10.93GDD405 pKa = 3.33VYY407 pKa = 11.7VNGFGYY413 pKa = 10.69LHH415 pKa = 7.17LGLLDD420 pKa = 3.63AVTINFTGHH429 pKa = 5.73GTDD432 pKa = 4.59DD433 pKa = 4.08DD434 pKa = 5.06VSGFWTIASTNFVDD448 pKa = 5.9ALIEE452 pKa = 4.16VQGTAIQRR460 pKa = 11.84ILYY463 pKa = 10.06CDD465 pKa = 4.15DD466 pKa = 4.14PVSQLKK472 pKa = 10.32CSQVAFDD479 pKa = 5.4LDD481 pKa = 3.89DD482 pKa = 3.81GFYY485 pKa = 10.38PISSRR490 pKa = 11.84NLLSHH495 pKa = 6.55EE496 pKa = 4.12QPISFVTLPSFNDD509 pKa = 3.26HH510 pKa = 6.27SFVNITVSASFGGHH524 pKa = 5.66SGANLIASDD533 pKa = 3.51TTINGFSSFCVDD545 pKa = 2.78TRR547 pKa = 11.84QFTISLFYY555 pKa = 11.08NVTNSYY561 pKa = 11.02GYY563 pKa = 10.2VSKK566 pKa = 10.92SQDD569 pKa = 3.46SNCPFTLQSVNDD581 pKa = 3.71YY582 pKa = 11.36LSFSKK587 pKa = 10.79FCVSTSLLASACTIDD602 pKa = 4.13LFGYY606 pKa = 9.44PEE608 pKa = 4.41FGSGVKK614 pKa = 8.38FTSLYY619 pKa = 10.0FQFTKK624 pKa = 11.13GEE626 pKa = 4.85LITGTPKK633 pKa = 10.48PLEE636 pKa = 4.31GVTDD640 pKa = 3.65VSFMTLDD647 pKa = 3.0VCTKK651 pKa = 7.34YY652 pKa = 9.42TIYY655 pKa = 10.66GFKK658 pKa = 10.88GEE660 pKa = 4.78GIITLTNSSFLAGVYY675 pKa = 7.31YY676 pKa = 10.55TSDD679 pKa = 3.25SGQLLAFKK687 pKa = 10.21NVTSGAVYY695 pKa = 10.52SVTPCSFSEE704 pKa = 3.9QAAYY708 pKa = 10.85VDD710 pKa = 4.13DD711 pKa = 6.26DD712 pKa = 3.81IVGVISSLSSSTFNSTRR729 pKa = 11.84EE730 pKa = 3.91LPGFFYY736 pKa = 10.78HH737 pKa = 7.56SNDD740 pKa = 2.92GSNCTEE746 pKa = 3.82PVLVYY751 pKa = 11.07SNIGVCKK758 pKa = 9.81SGSIGYY764 pKa = 9.24VPSQSGQVKK773 pKa = 9.94IAPTVTGNISIPTNFSMSIRR793 pKa = 11.84TEE795 pKa = 3.69YY796 pKa = 10.03LQLYY800 pKa = 6.15NTPVSVDD807 pKa = 3.13CATYY811 pKa = 9.95VCNGNSRR818 pKa = 11.84CKK820 pKa = 10.28QLLTQYY826 pKa = 9.3TAACKK831 pKa = 9.6TIEE834 pKa = 4.39SALQLSARR842 pKa = 11.84LEE844 pKa = 4.32SVEE847 pKa = 4.22VNSMLTISEE856 pKa = 4.32EE857 pKa = 4.08ALQLATISSFNGDD870 pKa = 3.29GYY872 pKa = 11.6NFTNVLGVSVYY883 pKa = 10.82DD884 pKa = 3.71PASGRR889 pKa = 11.84VVQKK893 pKa = 10.67RR894 pKa = 11.84SFIEE898 pKa = 4.23DD899 pKa = 3.54LLFNKK904 pKa = 9.39VVTNGLGTVDD914 pKa = 4.28EE915 pKa = 4.8DD916 pKa = 4.8YY917 pKa = 11.12KK918 pKa = 10.79RR919 pKa = 11.84CSNGRR924 pKa = 11.84SVADD928 pKa = 3.65LVCAQYY934 pKa = 11.29YY935 pKa = 9.11SGVMVLPGVVDD946 pKa = 3.59AEE948 pKa = 4.22KK949 pKa = 10.83LHH951 pKa = 6.51MYY953 pKa = 9.61SASLIGGMVLGGFTSAAALPFSYY976 pKa = 10.43AVQARR981 pKa = 11.84LNYY984 pKa = 10.03LALQTDD990 pKa = 4.07VLQRR994 pKa = 11.84NQQLLAEE1001 pKa = 4.79SFNSAIGNITSAFEE1015 pKa = 4.23SVKK1018 pKa = 10.42EE1019 pKa = 4.47AISQTSKK1026 pKa = 11.06GLNTVAHH1033 pKa = 7.25ALTKK1037 pKa = 9.6VQEE1040 pKa = 4.43VVNSQGAALTQLTVQLQHH1058 pKa = 6.2NFQAISSSIDD1068 pKa = 3.66DD1069 pKa = 3.63IYY1071 pKa = 11.74SRR1073 pKa = 11.84LDD1075 pKa = 3.15ILSADD1080 pKa = 3.79VQVDD1084 pKa = 3.36RR1085 pKa = 11.84LITGRR1090 pKa = 11.84LSALNAFVSQTLTKK1104 pKa = 8.56YY1105 pKa = 9.43TEE1107 pKa = 4.15VQASRR1112 pKa = 11.84KK1113 pKa = 9.06LAQQKK1118 pKa = 10.1VNEE1121 pKa = 4.45CVKK1124 pKa = 10.67SQSQRR1129 pKa = 11.84YY1130 pKa = 7.48GFCGGDD1136 pKa = 3.54GEE1138 pKa = 5.79HH1139 pKa = 6.85IFSLVQAAPQGLLFLHH1155 pKa = 6.22TVLVPSDD1162 pKa = 3.98FVDD1165 pKa = 4.92VIAIAGLCVNDD1176 pKa = 5.21EE1177 pKa = 3.94IALTLRR1183 pKa = 11.84EE1184 pKa = 4.29PGLVLFTHH1192 pKa = 6.92EE1193 pKa = 4.72LQNHH1197 pKa = 5.41TATEE1201 pKa = 4.39YY1202 pKa = 10.01FVSSRR1207 pKa = 11.84RR1208 pKa = 11.84MFEE1211 pKa = 3.62PRR1213 pKa = 11.84KK1214 pKa = 8.1PTVSDD1219 pKa = 4.03FVQIEE1224 pKa = 4.69SCVVTYY1230 pKa = 11.26VNLTRR1235 pKa = 11.84DD1236 pKa = 3.63QLPDD1240 pKa = 3.9VIPDD1244 pKa = 3.77YY1245 pKa = 11.31IDD1247 pKa = 3.25VNKK1250 pKa = 9.69TLDD1253 pKa = 4.12EE1254 pKa = 4.36ILASLPNRR1262 pKa = 11.84TGPSLPLDD1270 pKa = 3.87VFNATYY1276 pKa = 11.15LNLTGEE1282 pKa = 4.35IADD1285 pKa = 3.79LEE1287 pKa = 4.28QRR1289 pKa = 11.84SEE1291 pKa = 4.11SLRR1294 pKa = 11.84NTTEE1298 pKa = 3.87EE1299 pKa = 4.15LQSLIYY1305 pKa = 10.39NINNTLVDD1313 pKa = 4.54LEE1315 pKa = 4.17WLNRR1319 pKa = 11.84VEE1321 pKa = 5.97TYY1323 pKa = 10.41IKK1325 pKa = 9.23WPWWVWLIIFIVLIFVVSLLVFCCISTGCCGCCGCCCACFSGCCRR1370 pKa = 11.84GPRR1373 pKa = 11.84LQPYY1377 pKa = 8.78EE1378 pKa = 4.19VFEE1381 pKa = 4.36KK1382 pKa = 11.03VHH1384 pKa = 5.02VQQ1386 pKa = 3.02

Molecular weight: 151.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6WQB8|H6WQB8_9ALPC Envelope small membrane protein OS=Porcine epidemic diarrhea virus OX=28295 GN=E PE=3 SV=1
MM1 pKa = 7.63ASVSFQDD8 pKa = 3.2RR9 pKa = 11.84GRR11 pKa = 11.84KK12 pKa = 8.69RR13 pKa = 11.84VPLSLYY19 pKa = 10.66APLRR23 pKa = 11.84VTNDD27 pKa = 2.99KK28 pKa = 10.52PLSKK32 pKa = 10.59VLANNAVPTNKK43 pKa = 9.77GNKK46 pKa = 8.6DD47 pKa = 3.38QQIGYY52 pKa = 9.0WNEE55 pKa = 3.64QIRR58 pKa = 11.84WRR60 pKa = 11.84MRR62 pKa = 11.84RR63 pKa = 11.84GEE65 pKa = 4.48RR66 pKa = 11.84IEE68 pKa = 4.86QPSNWHH74 pKa = 6.49FYY76 pKa = 10.41YY77 pKa = 10.82LGTGPHH83 pKa = 6.47ADD85 pKa = 2.9LRR87 pKa = 11.84YY88 pKa = 7.7RR89 pKa = 11.84TRR91 pKa = 11.84TEE93 pKa = 3.79GVFWVAKK100 pKa = 9.55EE101 pKa = 4.01GAKK104 pKa = 9.42TEE106 pKa = 4.04PTNLGVRR113 pKa = 11.84KK114 pKa = 9.87ASEE117 pKa = 4.02KK118 pKa = 10.49PIIPNFSQQLPSVVEE133 pKa = 3.81IVEE136 pKa = 4.45PNTPPTSRR144 pKa = 11.84ANSRR148 pKa = 11.84SRR150 pKa = 11.84SRR152 pKa = 11.84GNGNNRR158 pKa = 11.84SRR160 pKa = 11.84SPSNNRR166 pKa = 11.84GNNQSRR172 pKa = 11.84GNSQNRR178 pKa = 11.84GNNQGRR184 pKa = 11.84GASQNRR190 pKa = 11.84GGNNNNNNKK199 pKa = 9.52SRR201 pKa = 11.84NQSKK205 pKa = 10.03NRR207 pKa = 11.84NQSNDD212 pKa = 3.15RR213 pKa = 11.84GGVTSRR219 pKa = 11.84DD220 pKa = 3.65DD221 pKa = 3.36LVAAVKK227 pKa = 10.47DD228 pKa = 3.79ALKK231 pKa = 10.92SLGIGEE237 pKa = 4.79NPDD240 pKa = 4.07KK241 pKa = 11.01LKK243 pKa = 10.46QQQKK247 pKa = 9.45PKK249 pKa = 10.0QEE251 pKa = 4.27RR252 pKa = 11.84SDD254 pKa = 3.7SSGKK258 pKa = 8.11NTPKK262 pKa = 10.27KK263 pKa = 9.59NKK265 pKa = 9.58SRR267 pKa = 11.84ATSKK271 pKa = 10.69EE272 pKa = 3.65RR273 pKa = 11.84DD274 pKa = 3.56LKK276 pKa = 10.98DD277 pKa = 3.0IPEE280 pKa = 3.86WRR282 pKa = 11.84RR283 pKa = 11.84IPKK286 pKa = 10.29GEE288 pKa = 4.08NSVAACFGPRR298 pKa = 11.84GGFKK302 pKa = 10.83NFGDD306 pKa = 3.81AEE308 pKa = 4.15FVEE311 pKa = 4.62KK312 pKa = 10.79GVDD315 pKa = 3.14ASGYY319 pKa = 9.79AQIASLAPNVAALLFGGNVAVRR341 pKa = 11.84EE342 pKa = 4.12LADD345 pKa = 3.7SYY347 pKa = 11.34EE348 pKa = 3.54ITYY351 pKa = 9.37NYY353 pKa = 10.28KK354 pKa = 8.23MTVPKK359 pKa = 10.33SDD361 pKa = 4.0PNVEE365 pKa = 4.18LLVSQVDD372 pKa = 3.54AFKK375 pKa = 10.5TGNAKK380 pKa = 9.27PQRR383 pKa = 11.84KK384 pKa = 8.83KK385 pKa = 9.71EE386 pKa = 4.06KK387 pKa = 9.23KK388 pKa = 9.21NKK390 pKa = 10.14RR391 pKa = 11.84EE392 pKa = 3.78TTQQLNEE399 pKa = 3.73EE400 pKa = 4.81AIYY403 pKa = 11.05DD404 pKa = 3.99DD405 pKa = 3.93VGVPSDD411 pKa = 3.56VTHH414 pKa = 7.56ANLEE418 pKa = 3.75WDD420 pKa = 3.83TAVDD424 pKa = 3.73GGDD427 pKa = 3.32TAVEE431 pKa = 4.31IINEE435 pKa = 4.01IFDD438 pKa = 3.69TGNN441 pKa = 2.86

Molecular weight: 48.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

6302

76

3974

1050.3

115.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.061 ± 0.393

2.92 ± 0.603

5.173 ± 0.437

3.951 ± 0.346

6.062 ± 0.674

7.061 ± 0.304

1.698 ± 0.233

4.967 ± 0.477

5.03 ± 0.813

9.092 ± 0.759

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.619 ± 0.362

5.522 ± 0.932

3.586 ± 0.366

3.078 ± 0.708

3.396 ± 0.977

7.839 ± 0.651

6.331 ± 0.487

10.235 ± 0.871

1.127 ± 0.339

4.253 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski