Gyrovirus 4
Average proteome isoelectric point is 7.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7DCG0|I7DCG0_9VIRU CA1 OS=Gyrovirus 4 OX=1214955 GN=VP1 PE=3 SV=1
MM1 pKa = 7.76 GSPDD5 pKa = 3.32 HH6 pKa = 6.91 RR7 pKa = 11.84 FSAVPIDD14 pKa = 5.03 LLDD17 pKa = 3.95 SVCPQWKK24 pKa = 8.77 QVCFNYY30 pKa = 10.66 GIACWLTEE38 pKa = 3.75 CRR40 pKa = 11.84 VSHH43 pKa = 6.65 SRR45 pKa = 11.84 VCRR48 pKa = 11.84 CTSFRR53 pKa = 11.84 NHH55 pKa = 5.54 WFQLEE60 pKa = 4.2 GKK62 pKa = 8.42 PVSTVEE68 pKa = 4.31 VGVQTDD74 pKa = 3.88 PGDD77 pKa = 3.91 LEE79 pKa = 4.39 PPRR82 pKa = 11.84 SGTSTAEE89 pKa = 3.2 IGPRR93 pKa = 11.84 VDD95 pKa = 2.8 AARR98 pKa = 11.84 RR99 pKa = 11.84 LLSSISFKK107 pKa = 10.72 RR108 pKa = 11.84 DD109 pKa = 2.47 ACRR112 pKa = 11.84 ALEE115 pKa = 4.21 GAPKK119 pKa = 9.63 PKK121 pKa = 9.68 KK122 pKa = 9.8 RR123 pKa = 11.84 KK124 pKa = 9.85 RR125 pKa = 11.84 GDD127 pKa = 3.02 ARR129 pKa = 11.84 KK130 pKa = 9.49 EE131 pKa = 3.36 FLRR134 pKa = 11.84 RR135 pKa = 11.84 FRR137 pKa = 11.84 EE138 pKa = 4.52 GEE140 pKa = 3.98 EE141 pKa = 4.29 TEE143 pKa = 5.94 SDD145 pKa = 3.98 AEE147 pKa = 3.88 WDD149 pKa = 4.37 SFVEE153 pKa = 4.34 WSDD156 pKa = 3.42 SDD158 pKa = 3.66 GDD160 pKa = 3.94 PGVDD164 pKa = 3.57 YY165 pKa = 10.32 MRR167 pKa = 11.84 GGAGGGILRR176 pKa = 11.84 EE177 pKa = 4.07 DD178 pKa = 3.47 RR179 pKa = 11.84 MGGGIEE185 pKa = 4.06 VEE187 pKa = 4.38 STGGGTGGTEE197 pKa = 3.65 GTPFLKK203 pKa = 10.65 LLSGNFTSSTPARR216 pKa = 11.84 MM217 pKa = 3.49
Molecular weight: 23.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.73
IPC2_protein 5.804
IPC_protein 5.779
Toseland 5.893
ProMoST 6.046
Dawson 5.97
Bjellqvist 5.995
Wikipedia 5.931
Rodwell 5.931
Grimsley 5.931
Solomon 5.97
Lehninger 5.957
Nozaki 6.198
DTASelect 6.364
Thurlkill 6.313
EMBOSS 6.275
Sillero 6.3
Patrickios 4.495
IPC_peptide 5.982
IPC2_peptide 6.275
IPC2.peptide.svr19 6.347
Protein with the highest isoelectric point:
>tr|I7DCG0|I7DCG0_9VIRU CA1 OS=Gyrovirus 4 OX=1214955 GN=VP1 PE=3 SV=1
MM1 pKa = 6.8 VRR3 pKa = 11.84 FRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 PWGRR11 pKa = 11.84 LYY13 pKa = 10.84 AWRR16 pKa = 11.84 RR17 pKa = 11.84 GGWHH21 pKa = 6.77 FKK23 pKa = 9.76 RR24 pKa = 11.84 RR25 pKa = 11.84 PYY27 pKa = 9.81 GRR29 pKa = 11.84 WYY31 pKa = 10.62 RR32 pKa = 11.84 SGKK35 pKa = 7.56 YY36 pKa = 8.05 RR37 pKa = 11.84 RR38 pKa = 11.84 GYY40 pKa = 9.25 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 5.78 RR44 pKa = 11.84 RR45 pKa = 11.84 YY46 pKa = 10.62 AFSKK50 pKa = 8.59 AFKK53 pKa = 9.67 RR54 pKa = 11.84 KK55 pKa = 9.52 FYY57 pKa = 9.72 IQHH60 pKa = 6.33 PGSYY64 pKa = 9.24 VVRR67 pKa = 11.84 KK68 pKa = 6.55 TRR70 pKa = 11.84 PYY72 pKa = 10.54 NSMKK76 pKa = 10.38 VFFQGVIFVNNAVFQSGQAEE96 pKa = 3.95 TSAFRR101 pKa = 11.84 VYY103 pKa = 10.55 SIEE106 pKa = 3.98 LSLRR110 pKa = 11.84 NLLLAYY116 pKa = 9.15 YY117 pKa = 8.72 VQYY120 pKa = 11.36 VSGGPGWTGTSAAKK134 pKa = 10.78 VNISPLDD141 pKa = 3.29 WWRR144 pKa = 11.84 WAYY147 pKa = 10.1 IRR149 pKa = 11.84 MHH151 pKa = 7.26 PSPDD155 pKa = 2.98 QRR157 pKa = 11.84 VFANATRR164 pKa = 11.84 PTDD167 pKa = 3.56 TQLFNYY173 pKa = 8.88 FSQWQLFRR181 pKa = 11.84 HH182 pKa = 6.16 IKK184 pKa = 9.02 TDD186 pKa = 3.9 FRR188 pKa = 11.84 VLNTDD193 pKa = 3.19 VTNTPDD199 pKa = 3.39 VRR201 pKa = 11.84 ILASLLTQDD210 pKa = 5.54 SYY212 pKa = 11.63 WQVQNKK218 pKa = 8.12 TSADD222 pKa = 3.36 LSQLKK227 pKa = 9.99 FPSFAEE233 pKa = 3.99 LTAWGAQWAFLPGQKK248 pKa = 9.72 SVSGRR253 pKa = 11.84 SFNHH257 pKa = 5.86 HH258 pKa = 5.96 SMRR261 pKa = 11.84 GTNDD265 pKa = 3.26 PKK267 pKa = 11.06 GEE269 pKa = 3.98 PWKK272 pKa = 9.49 TLTPLQHH279 pKa = 6.18 QVVSDD284 pKa = 3.7 INWVFGTLFLAEE296 pKa = 4.46 FSNFTVLNEE305 pKa = 3.95 YY306 pKa = 8.8 NTEE309 pKa = 3.75 NRR311 pKa = 11.84 ALAATGLSEE320 pKa = 3.7 TWSIVKK326 pKa = 9.48 VRR328 pKa = 11.84 SLFEE332 pKa = 4.18 FGNQQYY338 pKa = 11.3 LLADD342 pKa = 3.65 SVRR345 pKa = 11.84 FTSHH349 pKa = 7.21 LPTT352 pKa = 4.46
Molecular weight: 41.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 10.087
IPC_protein 10.906
Toseland 10.716
ProMoST 10.54
Dawson 10.862
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 11.008
Grimsley 10.935
Solomon 10.965
Lehninger 10.921
Nozaki 10.687
DTASelect 10.628
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.789
Patrickios 10.657
IPC_peptide 10.965
IPC2_peptide 9.589
IPC2.peptide.svr19 8.164
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
569
217
352
284.5
32.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.151 ± 0.39
1.23 ± 1.252
4.569 ± 1.469
4.569 ± 2.047
6.327 ± 1.078
8.436 ± 2.513
2.109 ± 0.456
2.636 ± 0.081
4.218 ± 0.044
7.206 ± 0.762
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.406 ± 0.274
3.691 ± 1.448
5.097 ± 0.561
4.042 ± 1.379
9.666 ± 0.007
8.612 ± 0.379
6.503 ± 0.321
6.503 ± 0.321
3.515 ± 0.759
3.515 ± 1.626
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here