Paenibacillus ferrarius

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7849 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V4HJR9|A0A1V4HJR9_9BACL ABC transporter OS=Paenibacillus ferrarius OX=1469647 GN=BC351_02950 PE=4 SV=1
MM1 pKa = 6.91FTITSYY7 pKa = 10.05TVEE10 pKa = 4.03LVKK13 pKa = 10.98DD14 pKa = 3.83PFQILTGQRR23 pKa = 11.84YY24 pKa = 8.26EE25 pKa = 3.88FLIDD29 pKa = 3.61IEE31 pKa = 4.3VEE33 pKa = 4.04EE34 pKa = 5.15DD35 pKa = 3.47DD36 pKa = 4.33EE37 pKa = 5.34LYY39 pKa = 10.8SEE41 pKa = 4.43SGLYY45 pKa = 9.67IRR47 pKa = 11.84VIYY50 pKa = 10.29NVEE53 pKa = 3.92EE54 pKa = 4.31AKK56 pKa = 10.82SGLLKK61 pKa = 10.91YY62 pKa = 10.22EE63 pKa = 4.28IYY65 pKa = 10.77EE66 pKa = 4.29KK67 pKa = 8.57TTQLYY72 pKa = 10.89LDD74 pKa = 4.87FEE76 pKa = 5.04LEE78 pKa = 4.16DD79 pKa = 3.71EE80 pKa = 5.02EE81 pKa = 4.49IASVEE86 pKa = 4.19AFCKK90 pKa = 10.17EE91 pKa = 3.88HH92 pKa = 6.21VQEE95 pKa = 4.54GNEE98 pKa = 3.87

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V4HHC3|A0A1V4HHC3_9BACL Cobalamin biosynthesis protein CbiX OS=Paenibacillus ferrarius OX=1469647 GN=BC351_30025 PE=4 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.8VLQARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7849

0

7849

2589470

26

4053

329.9

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.987 ± 0.035

0.736 ± 0.009

5.074 ± 0.021

6.193 ± 0.032

4.281 ± 0.023

7.023 ± 0.029

2.133 ± 0.015

7.047 ± 0.03

5.728 ± 0.028

10.1 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.016

4.209 ± 0.027

3.872 ± 0.018

4.122 ± 0.02

4.405 ± 0.024

6.681 ± 0.023

5.748 ± 0.036

6.955 ± 0.024

1.319 ± 0.01

3.593 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski