Lachnospiraceae bacterium KH1T2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4E4M0|A0A1I4E4M0_9FIRM WG containing repeat-containing protein OS=Lachnospiraceae bacterium KH1T2 OX=1855374 GN=SAMN05216390_10756 PE=4 SV=1
MM1 pKa = 7.52LKK3 pKa = 10.58KK4 pKa = 10.51NLIKK8 pKa = 10.76KK9 pKa = 10.17LGISAIAVFMAAMLITGCGNTVTDD33 pKa = 4.32SAPTTATSSTSVAEE47 pKa = 4.18ADD49 pKa = 3.75PSGEE53 pKa = 3.99VDD55 pKa = 3.8KK56 pKa = 11.57DD57 pKa = 3.78MPDD60 pKa = 3.21GQAPDD65 pKa = 3.86GQGGPGGQAPDD76 pKa = 3.79GQGGPGGQSSAPTEE90 pKa = 3.67YY91 pKa = 10.93AAANEE96 pKa = 4.24YY97 pKa = 10.3TSDD100 pKa = 3.64EE101 pKa = 4.44TVDD104 pKa = 3.77DD105 pKa = 4.23LTVEE109 pKa = 4.36STGSDD114 pKa = 3.14EE115 pKa = 4.7NAVHH119 pKa = 6.16VLNGSQVIFNNLMISRR135 pKa = 11.84NSSDD139 pKa = 3.14SSGGDD144 pKa = 2.95SASFYY149 pKa = 11.36GVGASLLTTDD159 pKa = 3.74GTAIVNGGTITSDD172 pKa = 3.27SKK174 pKa = 11.53GGAGLFAYY182 pKa = 10.28GDD184 pKa = 3.78GTIYY188 pKa = 10.42AQNVKK193 pKa = 9.73IDD195 pKa = 3.9TKK197 pKa = 10.78QDD199 pKa = 3.08TSGGVHH205 pKa = 6.49AAGGGTFYY213 pKa = 10.99GWDD216 pKa = 3.23LTVNTEE222 pKa = 4.38GEE224 pKa = 3.97SSAAIRR230 pKa = 11.84SDD232 pKa = 3.19RR233 pKa = 11.84GGGTMVIDD241 pKa = 4.31GGTYY245 pKa = 7.55TANGSGSPALYY256 pKa = 8.59CTADD260 pKa = 3.03ISVNNATLEE269 pKa = 4.23ATGSEE274 pKa = 4.9GVCLEE279 pKa = 4.04GLNTTRR285 pKa = 11.84LFDD288 pKa = 3.82TDD290 pKa = 4.57LTCDD294 pKa = 4.17MPDD297 pKa = 3.18QEE299 pKa = 5.02QNNNLSWSVIVYY311 pKa = 10.21QSMSGDD317 pKa = 3.83SEE319 pKa = 4.47VGAGTFDD326 pKa = 3.47MVGGSITSKK335 pKa = 10.82NGGLFYY341 pKa = 8.78TTNTEE346 pKa = 4.3SNFYY350 pKa = 10.67LSGVNITTADD360 pKa = 3.39DD361 pKa = 3.92CEE363 pKa = 4.44FFLRR367 pKa = 11.84ATGNANGRR375 pKa = 11.84GWGTTGEE382 pKa = 4.28NGADD386 pKa = 3.72CNFTADD392 pKa = 3.93DD393 pKa = 3.75QDD395 pKa = 3.56MKK397 pKa = 11.65GDD399 pKa = 3.91VQWDD403 pKa = 4.17SISKK407 pKa = 10.47LDD409 pKa = 3.86FYY411 pKa = 10.65MLNGSTLQGAFVDD424 pKa = 4.69DD425 pKa = 4.34EE426 pKa = 4.82SEE428 pKa = 4.11AGNGSDD434 pKa = 3.4EE435 pKa = 4.82GYY437 pKa = 11.2ANLYY441 pKa = 9.52IDD443 pKa = 5.5SSSTWTVTGDD453 pKa = 3.34STLTALHH460 pKa = 6.19NAGKK464 pKa = 10.14IVDD467 pKa = 3.76EE468 pKa = 4.81SGNSVTVKK476 pKa = 9.73GTDD479 pKa = 3.03GTVYY483 pKa = 10.8VEE485 pKa = 4.53GDD487 pKa = 3.42SEE489 pKa = 4.36YY490 pKa = 10.68TITVDD495 pKa = 5.11SYY497 pKa = 11.15DD498 pKa = 3.82TEE500 pKa = 6.2ADD502 pKa = 3.18TSEE505 pKa = 4.92ALTAPVWSDD514 pKa = 3.32YY515 pKa = 11.37AVEE518 pKa = 4.44KK519 pKa = 10.98PEE521 pKa = 4.21ALQQ524 pKa = 3.81

Molecular weight: 53.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4G327|A0A1I4G327_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium KH1T2 OX=1855374 GN=SAMN05216390_11566 PE=4 SV=1
MM1 pKa = 7.45WMTYY5 pKa = 7.14QPKK8 pKa = 9.66KK9 pKa = 8.41RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.87GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 8.94VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.66GRR39 pKa = 11.84AKK41 pKa = 10.06LTVV44 pKa = 3.04

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3398

0

3398

1154851

30

2372

339.9

38.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.431 ± 0.047

1.36 ± 0.016

6.33 ± 0.038

7.698 ± 0.051

4.308 ± 0.031

6.761 ± 0.039

1.629 ± 0.018

7.814 ± 0.041

7.436 ± 0.036

8.258 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.974 ± 0.019

4.983 ± 0.031

2.967 ± 0.022

2.482 ± 0.023

4.064 ± 0.033

6.395 ± 0.037

5.177 ± 0.033

6.74 ± 0.033

0.873 ± 0.015

4.319 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski